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Butterfly orchid anti-browning tissue culture method and anti-browning culture medium

The technology of a culture medium and a browning group is applied in the field of plant tissue culture, which can solve the problems of unenvironmental protection, incompleteness, and few anti-browning methods, and achieve the effects of cost saving, nutritional health, and low browning rate.

Inactive Publication Date: 2013-07-17
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is not difficult to find that these methods have certain defects and incompleteness and are not environmentally friendly. There are still very few studies on the anti-browning of the entire cultivation stage and a relatively systematic anti-browning method.

Method used

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  • Butterfly orchid anti-browning tissue culture method and anti-browning culture medium

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Embodiment Construction

[0039] The technical solution of the present invention will be further described below through specific embodiments.

[0040] The following example MS medium formula: KNO per liter of medium 3 (1900 mg / L), NH 4 NO 3 (1650 mg / L), MgSO 4 ·7H 2 O (370 mg / L), KH 2 PO 4 (170 mg / L), CaCl 2 (330 mg / L), KI (0.83 mg / L), H 3 BO 3 (6.2 mg / L), MnSO 4 ·H 2 O (16.9 mg / L), ZnSO 4 ·7H 2 O (8.6 mg / L), CuSO 4 ·5H 2 O (0.025 mg / L), CoCl 2 ·6H 2 O (0.025 mg / L), Na 2 MoO 4 ·2H 2 O (0.25mg / L), FeSO 4 ·7H 2 O (27.85mg / L), Na 2 EDTA (37.25 mg / L), glycine (2.0mg / L), niacin B3 (0.5mg / L), thiamine B1 (0.1mg / L), pyridoxine B6 (0.5mg / L), Inositol (100mg / L).

[0041] B5 medium formula: KNO 3 (2500 mg / L), (NH 4 ) 2 SO 4 (134 mg / L), NaH 2 PO 4 ·H 2 O (150 mg / L), MgSO 4 ·7H 2 O (250 mg / L), CaCl 2 (124 mg / L), KI (0.75 mg / L), H 3 BO 3 (3 mg / L), MnSO 4 ·H 2 O (10 mg / L), ZnSO 4 ·7H 2 O (2mg / L), CuSO 4 ·5H 2 O (0.025 mg / L), CoCl 2 ·6H 2 O (0.025 mg / L), Na 2 MoO 4 ·2H 2 O (0.25mg / L), FeSO 4 ·7H 2 O (27.85mg / L), Na 2 ...

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Abstract

The invention mainly relates to the field of plant tissue culture and particularly relates to a butterfly orchid anti-browning tissue culture method and an anti-browning culture medium. The tissue culture method comprises the steps of explant disinfection, primary culture, proliferation and differentiation culture, rooting culture, seedling hardening, transplanting and the like, wherein in the primary culture and the proliferation and differentiation culture, extracted solutions of calendulas, lemons, tomatoes, rosemary and the like are added into a corresponding culture medium so as to effectively prevent and treat a common browning problem in butterfly orchid tissue culture; and meanwhile, different types of cutting manners are used for helping to prevent and treat the browning problem at a differentiation phase, so as to form a compound anti-browning method. The method disclosed by the invention has the advantages of high stability, great success rate, greenness and environmental friendliness, and can annularly and repeatedly produce.

Description

Technical field [0001] The invention mainly relates to the field of plant tissue culture, in particular to a phalaenopsis anti-browning tissue culture method and an anti-browning culture medium. Background technique [0002] Browning of explants is an important problem encountered in plant tissue culture. Studies have shown that the main cause of browning is that polyphenol oxidase acts on the natural substrate phenols to form quinones. The addition of antioxidants and adsorbents can better control browning. [0003] In view of these causes of browning, earlier studies have shown that activated carbon can prevent browning, because the principle of activated carbon to prevent browning is the adsorption of activated carbon, and the adsorption of activated carbon itself is limited, that is It is impossible to add a large amount of activated carbon to each bottle, so the adsorption effect of a small amount of activated carbon is relatively small. Once the adsorbed harmful components a...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 项艳任洁沈周高赵康冯琳赵华琳
Owner ANHUI AGRICULTURAL UNIVERSITY
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