A kind of clematis tissue culture medium and its preparation method and application

Abstract

The invention discloses an anoectochilus roxburghii tissue culture medium, a preparation method thereof and an application of the anoectochilus formosanus tissue culture medium. According to the medium, 5g of agar, 20-28g of eclipta alba extracting solution, 15-23g of curculigo orchioides extracting solution, 12-20g of nacre mother of pearl and 3-10g of kaolin are added into each liter of MS medium. Eclipta alba is crushed, citric acid solution is added into the crushed eclipta alba, mixture is leached and extracted twice, and the mixture is concentrated to obtain the eclipta alba extracting solution. Curculigo orchioides are crushed, deionized water is added into the crushed curculigo orchioides, the mixture of the deionized water and the crushed curculigo orchioides is decocted, absolute ethyl alcohol is added into the mixture to perform soaking, and the mixture is decocted by slow fire, filtered and concentrated to obtain the curculigo orchioides extracting solution. The nacre mother of pearl is crushed, vinegar solution is added into the crushed nacre mother of pearl to perform soaking, and the soaked nacre mother of pearl is dried to obtain nacre mother of pearl powder. The agar, the eclipta alba extracting solution, the curculigo orchioides extracting solution, the kaolin and the nacre mother of pearl powder are added into the MS medium for ultrasound treatment to obtain the anoectochilus roxburghii tissue culture medium. The medium is reasonable in formula, good in sterilizing effect and can function in preventing browning, the survival rate and quality of anoectochilus roxburghii tissue culture seedlings are improved, environments cannot be polluted, cost is reduced, and the preparation method is simple in operation, good in repeatability and suitable for large-scale popularization and application.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a tissue culture medium for Auranthus clematis and its preparation method and application. Background technique [0002] Anoectochilus roxburghii (Anoectochilus roxburghii), also known as Golden Silk Grass, is a perennial herb of the family Orchidaceae. It is a traditional precious Chinese medicine in my country. It enjoys the laudatory title of "King of Medicine" among the people. In southern Zhejiang, Fujian, Taiwan, and Guangdong and Guangxi, clematis has been used to boil water, make tea, and make soup for a long time for physical fitness, nourishing and recuperation. Modern research shows that clematis contains polysaccharides, flavonoids, cardiac glycosides and other components, which can enhance human immunity, improve body resistance, prevent disease invasion, enhance and improve physical fitness and other effects. [0003] Most of the existing tissue cultur...

AI Technical Summary

Problems solved by technology

Earlier studies have shown that activated carbon can play an anti-browning effect, but the adsorption of activated carbon itself is limited, and the adsorption of a small amount of activated carbon is relatively small. Once the adsorbed harmful components reach saturation, there is basically no The effect is effective, and frequent replacement of the medium is not conducive to the effect of the nutrients in the medium, and there is a risk of contamination during the transfer process, and the effect of activated carbon on preventing browning has serious defects