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A method for rapid propagation of Phalaenopsis by leaf induction cluster buds

A phalaenopsis and leaf technology, which is applied to the field of rapid propagation of phalaenopsis, can solve the problems of low reproductive coefficient of phalaenopsis buds and high differentiation and variation rate of pseudo-protocorm, and achieve the advantages of shortening the rapid propagation time, great application value and considerable application prospect. Effect

Inactive Publication Date: 2011-12-21
深圳市深业农科花卉有限公司 +1
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AI Technical Summary

Problems solved by technology

[0003] Although the rapid propagation technology of Phalaenopsis has made some progress, the low reproductive coefficient of Phalaenopsis clustered buds, the special medium for differentiation of pseudoprotocorms, and the high rate of differentiation of pseudoprotocorms are all factors that restrict the industrial production of Phalaenopsis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The pedicel buds of Hanghu No. 1 safflower Phalaenopsis were wiped with 75% ethanol cotton balls, the pedicel bud bracts were peeled off, and the exposed pedicel buds were soaked in 0.1% mercury liter for 12 minutes, rinsed with sterile water and then inoculated. Induction medium: 1 / 3MS+BA3mg / L+NAA0.2mg / L+peptone 2g / L+coconut water 100ml / L+sucrose 20g / L. The culture conditions include a temperature of 25±2° C., a light time of 10-12 hours / day, and a light intensity of 1200-1500 Lx. After 48 days of cultivation, sterile seedlings were obtained.

[0019] Use sterile leaves to cut into sections, cut into squares with a diameter of about 1cm, scratch the edge of the leaves with a blade, and spread 14 pieces of leaves on the special medium for inducing cluster buds: Huabao No. 1 + BA5mg / L + NAA0.2mg / L++ peptone 2g / L+ coconut water 100ml / L+ sucrose 20g / L. The culture conditions include a temperature of 25±2° C., a light time of 10-12 hours / day, and a light intensity of 120...

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Abstract

The invention discloses a method for rapidly propagating Phalaenopsis by inducing clustered buds through leaves, in particular a method for sterilizing and cultivating Phalaenopsis pedicel buds to obtain aseptic materials, removing leaves from the aseptic materials, and germinating aseptic pedicel buds to obtain Cluster buds, while the cluster buds propagate, utilize aseptic materials or / and leaves in the cluster buds to induce cluster buds, and finally use the cluster buds as breeding objects to cultivate tissue culture seedlings to rapidly propagate Phalaenopsis. The method has the advantages of fast propagation speed, short growth cycle and reduced production cost.

Description

technical field [0001] The invention relates to a method for rapidly propagating Phalaenopsis through leaves, and the invention belongs to the tissue culture method of asexual reproduction. Background technique [0002] Phalaenopsis is a famous flower. It is called "Queen of Orchids" because of its beautiful and unique shape, huge flowers, bright colors, rich color and long flowering period. It is an epiphytic flower with uniaxial stems. It is difficult to satisfy the market demand with the propagation method of existing, and tissue culture is the most effective way of rapid propagation Phalaenopsis. In 1960, Morel proposed a method for in vitro clonal propagation of orchids. With the popularization of Phalaenopsis, the research on the rapid propagation system of Phalaenopsis is gradually deepening. Use mature fruit pods to carry out aseptic sowing to obtain seedlings; use sterile seedlings to induce pseudocorms or cluster buds; explants are sterilized to obtain cluster bu...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陈肖英徐明全郑平赵贵林姬澎侯学瑛张占路
Owner 深圳市深业农科花卉有限公司
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