The invention provides a method for preparing a
phalaenopsis plant tissue medium and relates to a
plant tissue culture technology. The method for preparing the
monstera deliciosa plant tissue medium adopts a
tissue culture technology, the formula of the medium consists of a seed
germination medium, a differential medium, a strong
seedling medium and a rooting medium, and the four media are respectively prepared and applied. The formula of the seed
germination medium comprises minimal medium Murashige and Skoog (MS), 6-benzyl
aminopurine, 1-naphthaleneacetic acid,
activated carbon,
cane sugar and
agar powder; the formula of the differential medium comprises minimal medium 1 / 3MS, the 6-benzyl
aminopurine, the 1-naphthaleneacetic acid, the
activated carbon, banana juice, the
cane sugar and the
agar powder; the formula of the strong
seedling medium comprises the minimal medium MS, the 6-benzyl
aminopurine, benzaole-3-
butyric acid, hyponex No.1, hyponex No.2, the
activated carbon, the
cane sugar and the
agar powder; the formula of the rooting medium comprises the minimal medium MS, the 1-naphthaleneacetic acid, the banana juice, the activated carbon, the can sugar and the agar powder; and the required medium products are respectively prepared and obtained according to the formulas, the
phalaenopsis plant tissue culturing and high power breeding are realized.