Tissue culture method of dendrobium officinale

Abstract

The invention relates to a tissue culture method of dendrobium officinale. The method comprises six steps of sprouting the dendrobium officinale into carmus, performing carmus proliferation and differentiation, performing dedifferentiation, strengthening seedlings, performing rooting and hardening seedlings. The dendrobium officinale is subjected to tissue culture by optimizing and improving the culture medium of each step; the used culture media are respectively an improved 1 / 2MS basic culture medium, an MS culture medium added with potato leaching powder, a 1 / 2MS culture medium added with potato leaching powder, an improved culture medium added with the potato leaching powder and NAA, an improved tissue culture method added with banana extracts, NAA and IBA comprises a dendrobium officinale seed germinating medium and an improved culture medium added with Hyponex No.1 and Hyponex No.2. The germinating rate of the dendrobium officinale is high; the germinating time is short; the growth speed of the tissue culture seedlings is accelerated; the time consumption is reduced; the cost is reduced.

Description

technical field [0001] The invention relates to the field of agricultural technology, in particular to a tissue culture method of dendrobium officinale. Background technique [0002] Dendrobium officinale Kimura et Migo is a perennial epiphytic herb of the genus Dendrobium orchidaceae. It is a precious Chinese medicinal material with a long history of application. It is listed as the first of the nine immortal grasses and is the gold in medicine. "Shen Nong's Materia Medica" records: "Dendrobium dendrobium removes numbness and lowers qi, nourishes the five viscera for deficiency, fatigue and thinness, strengthens yin and improves essence, and thickens the stomach after long-term use." Zhu Danxi, one of the four famous doctors in the Jin and Yuan Dynasties, said that Dendrobium has an outstanding effect of nourishing yin and strengthening yang, and that Dendrobium is unique in traditional Chinese medicine for nourishing yin. Throughout the ages, Dendrobium has been known as ...

AI Technical Summary

Problems solved by technology

In recent years, with the increasing awareness of the medicinal value of Dendrobium candidum, the demand for Dendrobium candidum has also continued to increase. Traditional wild resources can no longer meet people's demand, and even wild resources in some areas are facing depletion.

In order to better protect and utilize the resources of Dendrobium candidum, more and more scientific researchers have begun to respond to the call of the state and began to focus on the research and development of Dendrobium candidum, including artificial domestication and cultivation of Dendrobium candidum, asexual reproduction, etc., tissue culture technology of Dendrobium candidum It is an important means for the reproduction of Dendrobium candidum, and it is also a key way to vigorously promote the planting and propagation of Dendrobium candidum. However, in the general tissue culture process, the seedling growth cycle is long, which consumes a lot of resources and increases the production cost of tissue culture.

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