Method for rapidly propagating Camelia sinensis cv. Chongqing pipacha

A loquat tea and fast technology, which is applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of low reproduction coefficient, long reproduction cycle, and no information on loquat tea in vitro rapid propagation, etc., and achieve germination time. Short, fast multiplying effects

Inactive Publication Date: 2012-07-18
SICHUAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

All there is following defective in these two kinds of methods: (1) the cycle of propagation is long, and cutting propagation generally needs 12-14 months, and the cycle of seed propagation is longer; (2) reproduction coefficient is relatively low, especially cutting propagation; (3) It is not easy to maintain good characters by natural breeding of seeds; (4) easily subject to seasonal and geographical restrictions, because planting loquat tea requires sufficient moisture and suitable pH
[0004] Although there are a small amount of reports on the rapid propagation method of plants in the prior art, 1) there is no information about the in vitro rapid propagation of loquat tea at present; 2) in view of the genetic characteristics of each plant variety, the disclosed rapid propagation method cannot be used directly. In the rapid propagation of loquat tea

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Take the 1-2cm newly germinated stem section with buds on the cutting seedlings of loquat tea in the cultivation room, put it in soapy water, shake and wash it for 15 minutes, then soak it in 0.5% carbendazim for 15 minutes, rinse it with running water for 2 hours, and dry the explants with filter paper Remove the outer layer of the buds and the small leaves on the stems; first sterilize the explants with 70% alcohol on a sterile operating table for 30 seconds, then sterilize them with 0.08% mercury chloride for 9 minutes, and then sterilize the explants with sterile water. Rinse 4 times, cut off the base of the stem segment, then sterilize with 0.04% mercuric chloride for 3.5 minutes, rinse 4 times with sterile water; inoculate the sterilized explants in a 100ml Erlenmeyer flask with MS medium, Inoculate 1 explant in each bottle, inoculate 15 bottles each time, repeat 2 times, then put it into the tissue culture room, and cultivate it under the conditions of temperature...

Embodiment 2

[0026] Take the 1-2cm newly germinated stem section with buds on the loquat tea cutting seedlings in the cultivation room, put it in soapy water, shake and wash it for 18 minutes, then soak it in 0.5% carbendazim for 25 minutes, rinse it with running water for 3 hours, and dry the explants with filter paper Remove the outer layer of the buds and the small leaves on the stems; first sterilize the explants with 75% alcohol on a sterile operating table for 40 seconds, and then sterilize them with 0.12% mercury chloride for 11 minutes. Rinse 4 times, cut off the base of the stem segment, then sterilize with 0.06% mercuric chloride for 4 minutes, rinse 5 times with sterile water; inoculate the sterilized explants in a 100ml Erlenmeyer flask with MS medium, Inoculate 1 explant in a bottle, inoculate 15 bottles each time, repeat 2 times, then put it into the tissue culture room, and cultivate it under the conditions of temperature 23°C, light intensity 1200lx, and light 12h per day to...

Embodiment 3

[0028] Take the 1-2cm newly germinated stem section with buds on the loquat tea cutting seedlings in the cultivation room, put it in soapy water, shake and wash it for 20 minutes, then soak it in 0.5% carbendazim for 20 minutes, rinse it with running water for 1 hour, and dry the explants with filter paper Remove the outer layer of the buds and the small leaves on the stems; first sterilize the explants with 72% alcohol on a sterile operating table for 35 seconds, then sterilize them with 0.10% mercuric chloride for 10 minutes, and then sterilize the explants with sterile water. Rinse 5 times, cut off the base of the stem segment, then disinfect with 0.05% mercuric chloride for 3 minutes, rinse 5 times with sterile water; inoculate the sterilized explants in a 100ml Erlenmeyer flask with MS medium, One explant was inoculated in each bottle, 15 bottles were inoculated each time, repeated twice, and then placed in the tissue culture room, at a temperature of 25°C, with a light in...

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PUM

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Abstract

The invention discloses a method for rapidly propagating Camelia sinensis cv. Chongqing pipacha. The method comprises the following steps of: sequentially cleaning an explant of a stem segment with buds of an indoor cutting seedling of the Camelia sinensis cv. Chongqing pipacha by using soapy water, soaking in carbendazim, and washing by using running water, completely absorbing the moisture on the surface of the explant, and removing small leaves from outer layers of the buds and the stem segment; sequentially disinfecting the explant by using alcohol and mercury bichloride under aseptic conditions, washing by using sterile water, inoculating into a Murashige and Skoog (MS) culture medium, culturing, and screening out a survival aseptic material; transferring the aseptic material into a startup culture medium, culturing and inducing into buds; and transferring the germinant aseptic material into an enrichment culture medium, and continuing to culture to obtain cluster buds with the average multiplication coefficient of 3.31 to 4.86. By the method, the aseptic material of which the sterilization survival rate is up to 88.5 percent can be obtained, the germination time is short, the multiplication rate is high, the cluster buds of which the average multiplication coefficient is up to 4.86 can be obtained within three months, and excellent characters of a genotype and a phenotype of the original clone can be maintained.

Description

technical field [0001] The invention belongs to the technical field of rapid propagation of plants, and in particular relates to a method for rapid propagation of loquat tea. Background technique [0002] Loquat tea (Camelia sinensis cv. Chongqing pipacha) belongs to the evergreen trees of the genus Camelia (Theaceae) in the family Theaceae, and is mainly distributed in Chongzhou City, Dayi County and other areas in Sichuan Province. Loquat tea has a high yield and good quality. It is one of the provincial traditional fine varieties recognized by the Sichuan Provincial Tea Tree Breeding Committee, and has been rated as a geographical indication agricultural product of Sichuan Province. In addition, loquat tea has a large corolla, golden stamens, strong aroma, and a long flowering period, which is open almost throughout the winter, and has good ornamental value. [0003] At present, the propagation methods of loquat tea mainly rely on traditional seed propagation and cutting...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 唐琳陈放胡洪沙李晋宇
Owner SICHUAN UNIV
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