T cell serum-free medium and using method thereof
A technology of serum-free medium and basal medium, applied in the field of culture medium, can solve the problems of low T cell culture medium culture efficiency, potential safety hazards, animal serum protein residues, etc.
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Embodiment 1
[0048] The T cell serum-free medium in this embodiment includes basal medium and other additives, wherein the basal medium is mixed with DMEM, F12, and IMDM at a volume ratio of 3:3:1, and other additives are added to the basal medium Components, so that each liter of serum-free medium contains ethanolamine 0.1mmol, ketone sulfate 1μg, ferric nitrate 0.5mg, zinc sulfate 0.3mg, sodium selenite 20μg, sodium pyruvate 150mg, insulin 10mg, recombinant human transferrin 20mg, Glutamine 5ml, recombinant human serum albumin 0.5g, thioglycerol 20μmol, L-vitamin C 20mg, interleukin-2 5×10 4 IU.
Embodiment 2
[0050] The serum-free medium for T cells in this embodiment includes basal medium and other additives, wherein the basal medium is mixed with RPMI-1640 and IMDM at a volume ratio of 1:1, and other additives are added to the basal medium, So that each liter of serum-free medium contains ethanolamine 0.05mmol, ketone sulfate 1.5μg, iron nitrate 1mg, zinc sulfate 0.3mg, sodium selenite 15μg, sodium pyruvate 110mg, insulin 15mg, recombinant human transferrin 50mg, glutamine Amide 25ml, recombinant human serum albumin 5g, thioglycerol 60μmol, L-vitamin C 50mg, interleukin-2 1.5×10 5 IU.
Embodiment 3
[0052] The T cell serum-free medium in this embodiment includes basal medium and other additives, wherein the basal medium is mixed with DMEM, F12, and IMDM at a volume ratio of 3:3:1, and other additives are added to the basal medium Components, so that each liter of serum-free medium contains ethanolamine 0.1mmol, ketone sulfate 0.5μg, ferric nitrate 0.6mg, zinc sulfate 0.8mg, sodium selenite 1.8μg, sodium pyruvate 70mg, insulin 0.5mg, recombinant human trans Ferritin 6mg, glutamine 5ml, recombinant human serum albumin 0.5g, thioglycerol 65μmol, L-vitamin C 30mg, interleukin-2 5×10 4 IU.
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