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Anthurium tissue culture and fast propagation culture medium and anthurium tissue culture and fast propagation seed production method

An Anthurium group and culture medium technology, which is applied in the field of plant tissue culture research, can solve the problems of low sterilization difficulty, easy callus variation, and wound induction, etc., so as to shorten the culture period, reduce callus differentiation and variation, and improve the success rate effect

Inactive Publication Date: 2017-02-15
广州市名卉景观科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using leaves as explants, the difficulty of sterilization is relatively low, but some varieties are difficult to induce callus, and the callus is easy to mutate

Method used

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  • Anthurium tissue culture and fast propagation culture medium and anthurium tissue culture and fast propagation seed production method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A kind of Anthurium tissue culture rapid propagation seed production method, comprises the following steps:

[0026] S1. Sterilization of explants: rinse 3 times with sterile water, soak in 75% alcohol for 30 seconds, rinse 3 times with sterile water, cut off the browned part of the wound, soak in 0.1% mercury chloride for 5 minutes, rinse with sterile water;

[0027] S2. Callus induction culture: Under sterile conditions, the sterilized leaves were cut into 1cm×1cm size, inoculated in callus induction medium, and cultivated for 60 days in a dark environment at a temperature of 25 degrees Celsius. A callus with a size of 2mmX2mm can be obtained;

[0028] S3. Callus proliferation culture: inoculate the callus into the callus proliferation medium, and cultivate it for 40 days at a temperature of 25 degrees Celsius, a light intensity of 1500 Lux, and a light intensity of 10h / d to obtain a 1cmX1cm size of 1cmX1cm with adventitious bud points. Callus, if the callus is small...

Embodiment 2

[0041] A kind of Anthurium tissue culture rapid propagation seed production method, comprises the following steps:

[0042] S1. Sterilization of explants: rinse 3 times with sterile water, soak in 75% alcohol for 35 seconds, rinse 3 times with sterile water, cut off the browned part of the wound, soak in 0.1% mercury chloride for 8 minutes, rinse with sterile water;

[0043] S2. Callus induction culture: Under sterile conditions, the sterilized leaves were cut into 1cm×1cm size, inoculated in callus induction medium, and cultivated for 62 days in a dark environment at a temperature of 25 degrees Celsius. A callus with a size of 2mmX2mm can be obtained;

[0044]S3. Callus proliferation culture: inoculate the callus into the callus proliferation medium, and cultivate it for 45 days at a temperature of 25 degrees Celsius, a light intensity of 1800 Lux, and a light intensity of 10 h / d to obtain a 1cm×1cm size with adventitious bud points. Callus, if the callus is smaller, it can ...

Embodiment 3

[0057] A kind of Anthurium tissue culture rapid propagation seed production method, comprises the following steps:

[0058] S1. Sterilization of explants: rinse 3 times with sterile water, soak in 75% alcohol for 40 seconds, rinse 3 times with sterile water, cut off the browned part of the wound, soak in 0.1% mercury chloride for 10 minutes, rinse with sterile water;

[0059] S2. Callus induction culture: Under sterile conditions, the sterilized leaves were cut into 1cm×1cm size, inoculated in callus induction medium, and cultivated for 65 days in a dark environment at a temperature of 25 degrees Celsius. A callus with a size of 2mmX2mm can be obtained;

[0060] S3. Callus proliferation culture: inoculate the callus into the callus proliferation medium, and cultivate it for 50 days at a temperature of 25 degrees Celsius, a light intensity of 2000Lux, and a light of 10h / d to obtain 1cmX1cm size of 1cmX1cm with adventitious bud points. Callus, if the callus is smaller, it can c...

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Abstract

The invention discloses an anthurium tissue culture and fast propagation seed production method, which comprises the following steps: S1, explant sterilizing; S2, callus induction culture; S3, callus propagation culture; S4, callus differentiation culture; S5, successive transfer culture; S7, plantlet hardening; S8, transplanting. The invention provides an anthurium tissue culture and fast propagation culture medium, which comprises a callus induction culture medium, a callus propagation culture medium, a callus differentiation culture medium, a successive transfer culture medium and a rooting culture medium. According to the anthurium tissue culture and fast propagation culture medium and the anthurium tissue culture and fast propagation seed production method provided by the invention, the success rate of anthurium seed production is improved, the seed production period of anthurium tissue culture seedlings is shortened, and the aberration rate of callus differentiation is reduced. The yield and the quality of the anthurium tissue culture seedlings are improved; by utilizing a sterilization method, the pollution rate is effectively reduced; by utilizing a culture medium formula, the callus induction rate is high, the callus differentiation variation is low, the culture period is shortened, the probability of variation seedlings during successive transfer culture is reduced, the generation number of successive transfer culture is prolonged, and the yield is improved.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture research, in particular to an anthurium tissue culture rapid propagation medium and an anthurium tissue culture rapid propagation seed production method. Background technique [0002] The technical introduction generally adopted at present (relative to the existing technology of the same trade) anthurium (Anthuriumandraeanum) belongs to Araceae Anthurium, has another name called Anthurium flower, anthurium, and is a perennial epiphytic evergreen herb. Its flowers and leaves are beautiful and elegant, with bright red color, long flowering period, strong ornamental value and high ornamental value. It is one of the most popular rare flowers at home and abroad in recent years. At present, domestically, it is mainly propagated through tissue culture. Anthurium tissue culture rapid propagation is prone to undifferentiated callus, weak growth of tissue culture seedlings, and high mutation r...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 郑慈真朱桥明余结梅何文杰樊灿良邱培辉
Owner 广州市名卉景观科技发展有限公司
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