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Method for inducing corn haploid and multi-embryo using high oil type inducing series

A haploid and haploid induction system technology, applied in the fields of botanical equipment and methods, biochemical equipment and methods, applications, etc., can solve the problems of low frequency of multi-embryonic seedlings and unsatisfactory effects, and improve the induction Frequency, the effect of improving the efficiency of haploid discrimination

Inactive Publication Date: 2008-04-09
CHINA AGRI UNIV
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Problems solved by technology

However, the frequency of spontaneous generation of multi-embryonic seedlings is quite low. Yang Wenpeng et al. have carried out germination statistics on different types of maize, and the frequency of spontaneous generation of multi-embryonic seedlings in maize is only 0.058% (Yang Wenpeng, Bai Xiaoguang, Chen Zehui, Maize Double Embryo Preliminary observation of seedlings, [J] Guizhou Agricultural Sciences, No. 6, 1991)
Later, people used drugs (such as dimethyl sulfoxide), ion irradiation and other methods to induce polyembryonic seedlings, but the results were not satisfactory.
The method of obtaining a large number of polyembryonic seedlings by hybridization induction is rarely reported

Method used

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  • Method for inducing corn haploid and multi-embryo using high oil type inducing series

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Example 1. Induction of Haploid in Maize Using High Oil Haploid Inducer Line

[0022] 1. Experimental materials

[0023] High-oil haploid induction lines can be obtained from the National Maize Improvement Center of China Agricultural University.

[0024] Taking the high-oil type haploid induction line Nongda Gaoyouyu No. 2 as an example, its breeding method is shown in Figure 1:

[0025] Stock6 quoted from the American Maize Genetics Cooperative. North Agricultural University High Oil (BHO) is a high-oil improved population with a large embryo surface, and spontaneously produced haploids have been found in the field. In 1995, dozens of individual plants in the BHO population were selected for hybridization with Stock6, and the ear with the deepest Navajo mark in the grain was selected as the original combination of the selected line. Use this combination to backcross 1-2 times with Stock6 to form F 1 Self-crossing, one-time backcrossing (BC 1 ), two backcrosses (B...

Embodiment 2

[0045] Example 2. Induction of polyembryoids in maize using high-oil-type inducible lines

[0046] 1. Test materials

[0047] Gaoyouyu 2 can be obtained from the National Maize Improvement Center of China Agricultural University;

[0048] The high-oil hybrid 5598 can be obtained from the National Maize Improvement Center of China Agricultural University;

[0049] Hybrid Nongda 108 can be obtained from China Agricultural University;

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Abstract

The invention discloses a method using high-oil inducement series to induce the haploid and the polyembryony body of the corn. The method inducing the haploid and the polyembryony body of the corn provided by the invention is to use high-oil corn haploid inducement series as the father parent to pollinate the female parent, to obtain the first-generation grain and gain the haploid and the polyembryony body of the corn. The method not only has the advantages that the breeding process is simplified, the breeding course is accelerated, the purity of the breed is improved, etc. in the traditional haploid breeding; but also can use the genetic effect of the oil constituent to improve the identification efficiency of the haploid, and at the same can improve the inducement rate of the polyembryony body.

Description

technical field [0001] The invention relates to a method for inducing haploid and polyembryonic bodies of maize by using a high-oil type maize parthenogenesis inducing line. Background technique [0002] Maize is a monoecious, sexually reproducing crop. The maize breeding work mainly utilizes maize sexual reproduction to select maize inbred lines, prepare maize combinations, and reproduce maize hybrids. However, in the process of cultivating maize inbred lines, it must go through multiple generations of selfing and selection. It usually takes ten years to breed a variety, which consumes a lot of manpower, material resources and financial resources. Therefore how to shorten the breeding process and improve the breeding efficiency is the goal that breeders have been pursuing. [0003] Maize haploid is the product of gametophyte apomixis, including parthenogenesis and parthenogenesis. By artificially doubling the obtained haploids, homozygous diploids can be obtained in onl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/06A01H5/00C12Q1/68
Inventor 陈绍江宋同明黎亮
Owner CHINA AGRI UNIV
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