Method for suppressing browning of carya cathayensis sarg explant
A technology of explants and pecans, applied in the directions of botanical equipment and methods, horticultural methods, plant regeneration, etc., can solve the problems of uneven quality, long seedling period, hindering production and development, etc., and achieves inhibition of browning and operation. Simple and effective in inhibiting browning
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Embodiment 1
[0024] The method for suppressing the browning of hickory nut explants comprises the steps:
[0025] ① Disinfection treatment
[0026] Take the spring hickory tree with axillary buds as explants, wash it with running water for 1-2 hours, soak it with carbendazim (1000 times the weight of liquid) for 30 minutes, put it on a super-clean workbench, and wash it with 75% alcohol (volume percentage) Soak for 90s, wash with sterile water for 3 times, then soak with 0.1% mercuric chloride (mercuric chloride) for 15min, wash with sterile water for 3 times.
[0027] ②, culture medium preparation
[0028] Based on 1L (total) medium (pH adjusted to 5.8-6.0), the medium contains:
[0029] Catechin 3g.
[0030] The additional ingredients are: 6-Benzylaminopurine (6-Benzylaminopurine) 5mg, 6-furylmethyladenine (Kinetin, kinetin) 1mg, 2,4-Dichlorophenoxyacetic acid (2,4-Dichlorophenyloxy acetic acid) 0.1 mg, α-naphthaleneacetic acid (A-naphthlcetic acid) 0.05 mg, agar 7.5 g, sucrose 30 g....
Embodiment 2 and 3
[0036] In the culture medium, the catechins are 2g and 4g in turn, and the others are the same as in 1.
Embodiment 4
[0038] The additional components are: 4mg of 6-benzylaminopurine, 2.0mg of 6-furylmethyladenine, 0.05mg of 2,4-dichlorophenoxyacetic acid, 0.04mg of a-naphthaleneacetic acid, 8g of agar, and 25g of sucrose.
[0039] Others are with embodiment 1.
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