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Tissue culture and rapid propagation method for actinidia chinensis

A technology for fast propagation of Chinese kiwifruit and tissue culture, which is applied in the field of fast propagation of Chinese kiwifruit seedlings by tissue culture

Inactive Publication Date: 2014-02-05
SOUTHWEST FORESTRY UNIVERSITY
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Problems solved by technology

TDZ has a dual effect: it promotes the proliferation of axillary buds at low concentrations, and promotes the proliferation of adventitious buds and calluses at high concentrations (Murthy B N S, Murch S J, Saxena P K. Thidiazuron: A potent regulator of in vitro plant morphogenesis[J]. In Vitro Cellular & Developmental Biology – Plant , 1998,34(4): 267-275.), however, TDZ concentration was not involved in adventitious bud proliferation

Method used

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  • Tissue culture and rapid propagation method for actinidia chinensis
  • Tissue culture and rapid propagation method for actinidia chinensis
  • Tissue culture and rapid propagation method for actinidia chinensis

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Embodiment Construction

[0037] The leaves of Actinidia sinensis 'Hort16A' and the fine line 'Xilinxuan 1' selected from it were used as tissue culture materials below. Since the process steps and medium conditions are the same, the leaf tissue culture process of the two varieties will not be described separately.

[0038] The leaves were soaked in 0.1% washing solution containing 2 drops of Tween for 1 min, and then rinsed with running water for 2 h for later use. exist

[0039] On the ultra-clean workbench, soak it in 75% alcohol for 60s, then immerse it in 0.1% mercuric chloride, take it out after 20 minutes, and soak it repeatedly with sterile water 4 times before use. The leaves were cut into 1cm×1cm blocks along the main veins, and bluntly placed in the callus induction and differentiation medium, the medium was MS+0.3mg / L ZT+0.05 mg / L NAA; cultured in the dark for 3 weeks, Light for another 2 weeks. The culture room is illuminated for 16h / d, the light intensity is 2000LX, the temperature...

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Abstract

The invention discloses a tissue culture and rapid propagation method for actinidia chinensis, and relates to the tissue culture and rapid propagation method for actinidia chinensis seedlings. According to the method, young leaves of the current year serve as explants and are inoculated on a medium MS+0.3mg / LZT+0.05mg / LNAA subjected to callus induction and cluster bud differentiation. The invention also provides an improved MS; after cluster buds are differentiated, the cluster buds are sub-cultured in an improved MS+2mg / LZT proliferation culture medium, and are transferred into an improved MS+0.015mg / LTDZ thickening culture medium according to seedling height; small seedlings which are in accordance with rooting are transferred into 1 / 2 of improved MS+0.7mg / LIBA+0.1g / LAC rooting culture medium; the small seedlings are transplanted and are domesticated after roots grow. By the method, the callus induction rate reaches 96.4 percent; the height of cluster bud reaches 1.67cm; the proliferation coefficient of cluster buds reaches 3.67; the rooting rate reaches 98.12 percent; moreover, the culture medium is low in cost and short in propagation period, and industrial production can be realized.

Description

technical field [0001] The invention relates to a rapid propagation method of plant seedling tissue culture, in particular to a rapid propagation method of Chinese kiwifruit seedling tissue culture. Background technique [0002] Kiwifruit (kiwifruit) belongs to Actinidiaceae (Actinidiaceae) Actinidiaceae ( Actinidia spp.) is a deciduous vine, an important economic tree and fruit tree. Kiwi fruit is rich in nutrients such as Vc, minerals and anthocyanins (Singletary, 2012), and has high nutritional and medicinal value. In recent years, it has been widely used in edible forms such as fresh fruit, beverages, and health care products, and the market demand is relatively large (Duan Dongyang et al. Research on the therapeutic value of kiwifruit and the mechanism of discoloration during processing and control measures [C]. / / Chinese Horticultural Society Tropical Proceedings of the Inaugural Conference of the South Subtropical Fruit Branch and the First Academic Symposium. 2006:...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 张汉尧张太奎刘小珍
Owner SOUTHWEST FORESTRY UNIVERSITY
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