Method for rapid tissue propagation and breeding seedling of bletilla striata seeds
A technology for rapid propagation of seedlings and seeds, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve problems such as difficult to meet mass cultivation, long division propagation cycle, low reproduction efficiency, etc., to achieve shortening tissue culture propagation cycle, shorten production time, and reduce production cost
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Embodiment 1
[0024] (1) Preparation and sterilization of Bletilla striata seeds: Take uncracked and robust Bletilla striata capsules, wash them with washing powder for 1-2 minutes, rinse them with running water, and soak them in 70% ethanol for 30s on an ultra-clean workbench , and then soaked in 0.15% mercuric chloride for 20 minutes, rinsed with sterile water for 4-5 times, put it on sterilized absorbent paper, and blot the water for later use.
[0025] (2) Seed germination and protocorm induction: After the seeds are sterilized, they can be inoculated on the protocorm induction medium, and the temperature is controlled at 22-24°C during the day and 18-20°C at night, in a dark box Carry out seed cultivation, after 5-6 days, the seeds begin to expand and increase. At this time, adjust the cultivation conditions, the temperature is controlled at 25±2°C, the light intensity is 1500-2000Lx, and the light is 12 hours a day. Protocorms are formed after 10 days.
[0026] (3) Protocorm prolifera...
Embodiment 2
[0031] (1) Preparation and sterilization of Bletilla striata seeds: Take uncracked and robust Bletilla striata capsules, wash them with washing powder for 1-2 minutes, rinse them with running water, and soak them in 70% ethanol for 30s on an ultra-clean workbench , and then soaked in 0.15% mercuric chloride for 20 minutes, rinsed with sterile water for 4-5 times, put it on sterilized absorbent paper, and blot the water for later use.
[0032] (2) Seed germination and protocorm induction: After the seeds are sterilized, they can be inoculated on the protocorm induction medium, and the temperature is controlled at 22-24°C during the day and 18-20°C at night, in a dark box Carry out seed cultivation, after 5-6 days, the seeds begin to expand and increase. At this time, adjust the cultivation conditions, the temperature is controlled at 25±2°C, the light intensity is 1500-2000Lx, and the light is 12 hours a day. Protocorms are formed after 10 days.
[0033] (3) Protocorm prolifera...
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