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Sexual propagation method of bletilla striata

A technology of sexual reproduction and Bletilla striata, applied in the field of sexual reproduction of Bletilla striata, can solve problems such as difficulty in meeting large-scale production, low natural seed germination rate, low reproduction coefficient, etc. Stable effect

Inactive Publication Date: 2015-03-25
湖北济世药业有限责任公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The natural germination rate of Bletilla striata seeds is extremely low, and reproduction is difficult. Artificial breeding is a powerful measure to meet the market of Bletilla striata. The current conventional methods are mostly based on division, that is, the tubers are cut into small pieces for planting. The reproduction coefficient is low, and it is difficult to meet large-scale production needs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 : the sexual reproduction method of bletilla striata, concrete steps are as follows:

[0021] (1) Treatment of fruit pods:

[0022] Collect ripe fruit pods of Bletilla striata, wash with water, and dry naturally;

[0023] Surface disinfection: soak and disinfect with 75% alcohol for 10 minutes, then rinse with sterile water;

[0024] Mercury chloride disinfection: soak and disinfect with 0.1% mercury chloride solution for 5 minutes, then rinse with sterile water;

[0025] Further disinfection: light the alcohol lamp, clamp the fruit pods with tweezers, and dry the surface moisture of the fruit pods with the outer flame of the alcohol flame;

[0026] (2) Preparation of nutrient base: According to the mass percentage, mix 5% banana puree and 95% humus to obtain natural nutrients; then, add 1 mg of naphthaleneacetic acid per liter of natural nutrients; Sterilize at a temperature of 121°C for 30 minutes to obtain a nutrient base;

[0027] (3) Inoculation and ...

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Abstract

The invention provides a sexual propagation method of bletilla striata. The sexual propagation method of bletilla striata particularly comprises the steps of processing of capsules, preparation of nutrients, inoculation and cultivation, and bottle out domestication, and is characterized in that in the step of processing of capsules, an alcohol lamp drying mode replaces a filter paper sucking dry mode in the prior art; in the step of preparation of nutrients, combination of banana slurry and humus is adopted; in the step of inoculation and cultivation, only inoculation, germination cultivation and strong seedling cultivation steps are included, the conventional enlargement cultivation process in an existing tissue culturing technology is omitted, and the process is simplified and the cost is lowered while the purposes are achieved; in the step of bottle out domestication, cultivation is performed through humus in a greenhouse, domestication is finished under the condition of controlling the relative humidity, temperature and shading rate, and qualified bletilla striata seedlings are obtained. Bletilla striata seeds are used for scale seedling culturing, the survival rate is high, manual scale propagation of bletilla striata is achieved, and the obtained bletilla striata seedlings are low in contamination rate, high in differentiation rate and stable in inheritable character.

Description

technical field [0001] The invention belongs to the field of planting Chinese medicinal materials, and in particular relates to a method for sexual reproduction of bletilla striata. Background technique [0002] Bletilla striata is a perennial herbaceous plant of the genus Bletilla striata in the family Orchidaceae. It is a commonly used medicinal material, and its tubers are used as medicine. [0003] For a long time, the bletilla striata market has mainly relied on wild resources, which have been severely exploited, coupled with the destruction of the ecological environment, and wild resources are becoming increasingly depleted. The natural germination rate of Bletilla striata seeds is extremely low, and reproduction is difficult. Artificial breeding is a powerful measure to meet the market of Bletilla striata. The current conventional methods are mostly based on division, that is, the tubers are cut into small pieces for planting. The reproduction coefficient is low, and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00
CPCA01G31/00
Inventor 曹克红孙良学
Owner 湖北济世药业有限责任公司
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