Fast propagation method for saccharum arundinaceum

Abstract

The invention provides a fast propagation method for saccharum arundinaceum. The fast propagation method comprises the following steps: I, selecting explants and sterilizing the explants; II, sterilizing treated seeds, inoculating the sterilized seeds on a callus-inducing medium, inoculating ten explants in each culture flask, and adjusting the pH value of the culture medium to be 5.8, and carrying out dark cultivation at 25 DEG C, wherein calluses are formed after 5 to 7 days' cultivation; III, under the dark cultivation condition, peeling off embryonic calluses which are cultivated on the callus culture medium, transferring the embryonic calluses onto a subculture culture medium for cultivation, and adjusting the pH value of the subculture culture medium to 5.8, wherein the temperature is 24 to 26 DEG C, and each proliferation cycle lasts for 20 to 28 days; IV, differentiating buds; V, rooting and proliferating; VI, transplanting. The fast propagation method provided by the invention has the advantages that the sampling is simple and convenient; the inductivity of a cultivated callus is high; the differentiation rate, the proliferation coefficient and the stability are high; a large quantity of tube seedlings which grow healthily can be obtained in a relative short time. The fast propagation method has the advantages that the maneuverability is high; the production cost is low; the application value is high; the suitability for industrialized production is very high.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, in particular to a method for rapid propagation of sarcophagus. Background technique [0002] Spotted grass (Saccharumarundinaceum (Retz) Jeswiet.) is a perennial, densely bushy tall herb of the genus Saccharum of the Poaceae (Poaceae) family (Sorghum family) (Sorghum family) (Andropogoneae). It is a C4 plant with tall plants and wild plant heights. More than 4 meters, or even 6-7 meters, the stem is about 2 cm thick, the root system is developed, it is resistant to barrenness and drought, and it is friendly to the environment. It grows well on hilly slopes, river banks, roadsides and gravel beaches. In tropical and subtropical my country Widely distributed in warm temperate regions, India, Myanmar, Thailand, Vietnam, Malaysia and other countries also have rich wild resources. Because of its excellent botanical characteristics, it has been widely used in the genetic improvement of cultiv...

AI Technical Summary

Problems solved by technology

But, the defective that this cultivation method exists is:

[0010] This scheme has high sampling requirements and is difficult to operate. Inadequate sampling can easily lead to callus induction failure and increase production costs.

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