30results about How to "High value-added coefficient" patented technology

The invention provides a tissue culture method of ilex verticillata. The tissue culture method comprises the following steps: (1) selecting an explant from a female plant; (2) processing the explant, namely sterilizing the explant; (3) performing inducing culturing on buds; (4) culturing the buds in enrichment; and (5) performing inducing culturing on roots. The tissue culture method disclosed by the invention is high in survival rate and high in reproduction speed, the growth coefficient achieves 6.1, the rooting percentage achieves 95.2%, and the ilex verticillata female young plant with pure quality is obtained. The method is free from season limitations, and can be used for rapidly propagating the ilex verticillata throughout the year, so that the large production of the ilex verticillata is realized, and an available method is provided for the rapid extension of the ilex verticillata.

The invention discloses a detoxification method of lily bulbs, wherein the method comprises the following steps: (1) placing the lily bulbs for 40-50 days at the temperature of 3-8 DEG C; (2) selecting the lily bulbs having the bud length of 4-8 cm, stripping outer-layer flakes, leaving bulbus discs with 3-5 inner-layer flakes, placing the bulbus discs into an incubator, and preprocessing, wherein the preprocessing conditions comprise that the illumination intensity is 1500-2000 Lux, the temperature is 35-40 DEG C and the processing time is 2-5 days; (3) selecting a normally growing bulbus disc, stripping a 0.4-0.7 cm stem tip, transferring into a differentiation culture medium, firstly, carrying out dark culture for 1-2 days at the temperature of 23-25 DEG C, then transferring into an illumination incubator with the illumination intensity of 1500-2000 Lux and the illumination time of 12-16 h / d, heating up at a speed of 1-5 DEG C / d, rising the temperature to 35-45 DEG C, and then keeping the temperature and culturing for 35-45 days. Lily bulb viruses are removed by combining heat treatment and chemical treatment, the induction culture period is shortened, and the method also has the advantages of high detoxification rate, high value-added coefficient and the like, and provides a technical basis for culture of lily virus-free plants and promotion of virus-free culture research.

The invention discloses a fast propagation method of tissue culture of traditional Chinese medicine Artemisia annua. The fast propagation method of tissue culture of traditional Chinese medicine Artemisia annua is that culture a terminal bud or an axillary bud of Artemisia annua are cultured in an initial medium so as to obtain an aseptic seedling. The initial medium is a medium of MS medium added with 6-BA and GA. The final concentration of the 6-BA is 0.1 to 2.0 mg / l and the GA is 0.2 to 2.0mg / l. The invented method has the advantages of easy avail of materials and aseptic materials, low variation rate, high enhancement factor, 5 to 6 times of multiple in 4 weeks, over 93 percent of rooting rate of tissue culture seedlings, over 95 percent of survival rate of transplanting and very strong commercialized production capacity. The high productive Chinese medicine Artemisia annua is reproduced by making use of the invention, and the high arteannuin of the offspring can be stabilized, thus increasing the output of arteannuin.

The invention discloses a stable and high-efficiency method for anti-browning and tissue culture multiplication of Tokyo Sizhaohua, which comprises the following steps: 1) Taking materials: in fine weather from April to May in spring, the vegetative branches that were born in the year without disease and grew vigorously ; 2) Sterilization: The sterilization method is a combination of alcohol 30s + 0.1% mercury chloride for 7 minutes; 3) Aseptic anti-browning system establishment: the sterilized stem segments are spread out on the sterilized filter paper to dry, and inoculated in a seed containing 200mg· In the culture medium of L-1 PVP anti-browning agent, wait for the buds to grow to 3-4cm, and carry out proliferation culture; 4) Proliferation culture: inoculate the induced buds in the proliferation medium (WPM+1.0 mg·L-16- BA+0.05 mg·L-1NAA), subculture every 20-25 days. The invention optimizes each stage of tissue culture and multiplication of Tokyo Sizhaohua, effectively reduces the browning rate to 17.87%, and increases the multiplication coefficient up to 3.09.

The invention discloses a tissue culture rapid propagation method of Guangdong anoectochilus roxburghii, which selects an axillary bud or a terminal bud of anoectochilus roxburghii in the Guangdong area in China as an explant and obtains a mass of vegetative propagated progeny plants by inducing the adventitious buds, carrying out proliferation and carrying out rooting cultivation. By adopting the method of the invention, one axillary bud can be induced to generate about 2 to 4 sprouts, after two months, the proliferation coefficient is between 3 and 4, and the rooting rate can reach over 90 percent. Therefore, the method greatly improves the seedling production capacity of Guangdong anoectochilus roxburghii, shortens the seedling culture time, saves the planting cost and provides the technical guarantee for the storage of the genetic resources of Guangdong anoectochilus roxburghii.

The invention discloses an induction method for a grape fruit callus and a culture medium thereof. The culture medium comprises the composition: a basal culture medium, 0.8-1.2 mg / L of TDZ, 0.3-0.5 mg / L of IBA, 18-22 g / L of sugar, and 5-7 g / L of agar. The induction method is characterized by comprising the steps: sterilizing grape fruits, then peeling, taking pulp blocks, inoculating the inductionculture medium with the pulp blocks, and carrying out induction culture until the callus is formed. The culture method of the fruit callus of 'Xiahei' grapes is successfully established, and the induction rate of the fruit callus reaches 100%, a new way is provided for molecular biology and genetic engineering research of the 'Xiahei' grapes, a new experimental material is provided for the genetic engineering research and gene transformation of the grapes, and meanwhile, a reference is also provided for culture of the grape fruit callus.

The present invention provides a kind of rapid propagation method of schizomaea, comprising step 1: explant selection and sterilization; step 2, sterilized seeds are inoculated on the callus induction medium, and each bottle is inoculated with 10 Block explants, adjust the pH of the medium to 5.8, place them in dark culture at 25°C, and start to form callus after 5 to 7 days of culture; step 3, under dark culture conditions, the cultured on the callus medium Embryogenic callus, after peeling off, transferred to the subculture medium for culture, the pH of the medium was adjusted to 5.8, the temperature was 25±1°C, and every 20d to 28d was a multiplication cycle; step 4: bud differentiation, step 5: rooting and Proliferation; Step six: transplanting. The method for propagating the grass grass provided by the invention has the advantages of simple sampling, high induction rate of the cultured callus, high differentiation rate, high value-added coefficient, good stability, and can obtain a large amount of robust test-tube plantlets in a relatively short period of time, and The method has strong operability, low production cost and high application value, and is very suitable for industrial production.

The invention relates to the technical field of tissue culture, in particular to a medium and a method for tissue culture of euonymus maackii. The tissue culture medium provided by the invention is applicable to a variety, namely 'Jinzhi Yuye' (euonymus macckii rupr.), of the euonymus maackii; on the basis of a 1 / 2MS medium, KT, NAA, TDZ and gibberellin are added for promoting callus induction; on the basis of an MS medium, KT and TDZ are added for promoting axillary bud induction; and a WPM medium is taken as a seedling hardening medium. Induced by the medium provided by the invention, a growth coefficient can be increased to 9.72, and a survival rate of seedlings can reach 93% or above.

The invention discloses a method for inducing embryoid bodies of cabbage, which includes induction of sterile seedlings, induction of embryoid bodies, induction of regenerated plants, seedling hardening and transplantation. directly induce regeneration plants or induce embryoid bodies or induce budding, and the induction rate is not high; the following hypocotyls of the present invention are used as explants for inducing embryoid bodies, and the induction rate can reach 90%, and the embryoid bodies induce regenerated plants. The induction rate can reach 90%; the regenerated plants are induced by embryoid bodies, and the regenerated plants have high genetic stability, no physiological isolation from the mother body, and a high value-added coefficient.

The invention provides a method for breeding wenshan paphiopedilum seedlings by using somatic embryo. After mature wenshan paphiopedilum fruits are sterilized, the fruits are cut apart to obtain seeds, the seeds are inoculated in an induced germination medium to obtain young embryo, and the young embryo is inoculated in a liquid nutrient medium to obtain the somatic embryo; differentiation seedlings are obtained through differentiation cultivation; the differentiation seedlings are inoculated in a root medium to perform root cultivation, and finally the seedlings are exercised and transplanted, namely the wenshan paphiopedilum seedlings are obtained. The method for breeding wenshan paphiopedilum seedlings by using the somatic embryo obtains the young embryo after sterile germination of the wenshan paphiopedilum mature fruits, breaks dormancy of shoots, promotes germination of the seeds and growth of callus, performs rapid cultivation on the plant somatic embryo, enables wenshan paphiopedilum tissue cultivation enhancement factor to be improved to above 5 times from original 1 time, and greatly improves reproduction efficiency of the wenshan paphiopedilum. Survival rate of the wenshan paphiopedilum seedlings after transplanting reaches above 90%.