Method for performing tissue culture of dendrobium candidum seedlings
A technology of Dendrobium officinale and tissue culture, applied in the field of plant biology, can solve the problems of low reproduction rate, difficult mass production and industrial production, slow differentiation, etc., and achieve the effects of promoting rapid growth, easy management, and less pests and diseases
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Embodiment 1
[0023] A kind of method for dendrobium candidum seedling tissue culture that the present invention proposes, comprises the steps:
[0024] S1. Sterilization: UV sterilize the inoculation room and inoculation instruments;
[0025] S2, germination: select qualified capsules of Dendrobium officinale, disinfect the capsules, soak the surface of the capsules with 95% medical alcohol, cut the disinfected capsules longitudinally, take out the seeds with tweezers and evenly scatter them in a container filled with germination nutrient solution. Seed germination culture is carried out in the culture bottle, the culture temperature is 23°C, the culture light intensity is 1800Lux, the culture light time is 12h / d, and the average disinfection frequency is 1.5 times / day. After 35 days of culture, green protocorms are formed, and the germination nutrient solution The components include: manganese sulfate 0.012g / L, zinc sulfate 6.2×10 -3 g / L, copper sulfate 5.4×10 -5g / L, ferrous sulfate 0.0...
Embodiment 2
[0028] A kind of method for dendrobium candidum seedling tissue culture that the present invention proposes, comprises the steps:
[0029] S1. Sterilization: Ozone sterilizes the inoculation room and inoculation equipment;
[0030] S2, germination: select qualified capsules of Dendrobium officinale, disinfect the capsules, soak the surface of the capsules with 95% medical alcohol, cut the disinfected capsules longitudinally, take out the seeds with tweezers and evenly scatter them in a container filled with germination nutrient solution. Seed germination culture is carried out in the culture bottle, the culture temperature is 24°C, the culture light intensity is 1500Lux, the culture light time is 13h / d, and the average disinfection frequency is 1 time / day. After 40 days of culture, green protocorms are formed, and the germination nutrient solution The components include: manganese sulfate 0.007g / L, zinc sulfate 6.4×10 -3 g / L, copper sulfate 5.3×10 -5 g / L, ferrous sulfate 0.0...
Embodiment 3
[0033] A kind of method for dendrobium candidum seedling tissue culture that the present invention proposes, comprises the steps:
[0034] S1. Sterilization: UV sterilize the inoculation room, and ozone sterilize the inoculation equipment;
[0035] S2, germination: select qualified capsules of Dendrobium officinale, disinfect the capsules, soak the surface of the capsules with 95% medical alcohol, cut the disinfected capsules longitudinally, take out the seeds with tweezers and evenly scatter them in a container filled with germination nutrient solution. Seed germination culture is carried out in the culture bottle, the culture temperature is 23°C, the culture light intensity is 1600Lux, the culture light time is 12h / d, and the average disinfection frequency is 1.4 times / day. Green protocorms are formed after 38 days of culture, and the germination nutrient solution The components include: manganese sulfate 0.01g / L, zinc sulfate 6.30×10 -3 g / L, copper sulfate 5.36×10 -5 g / L,...
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