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A tissue culture method using Pan's Bingdengyulu inflorescence axis as explant

A tissue culture and ice lamp yulu technology is applied to the tissue culture of the succulent Pan's ice lamp yulu, and the tissue culture field with the inflorescence axis of Pan's ice lamp yulu as explants, so as to achieve a large value-added coefficient, improve callus induction rate, The effect of meeting production needs

Active Publication Date: 2022-05-10
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in order to improve reproduction efficiency and reduce costs, indicators such as callus induction rate and rooting rate need to be further improved

Method used

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  • A tissue culture method using Pan's Bingdengyulu inflorescence axis as explant
  • A tissue culture method using Pan's Bingdengyulu inflorescence axis as explant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] A kind of tissue culture method taking Pan's Bingdengyulu inflorescence axis as explants, it comprises the steps:

[0044] 1. Selection and disinfection of explants: At the end of March, the inflorescence rachis of Yulu in full flowering stage was selected as explants. The disinfection method was to rinse with running water for 30 minutes, then disinfect with 75% ethanol for 30 seconds on a sterile operating table, and then use 0.1% The mercuric chloride was sterilized for 8 minutes, and finally washed 6 times with sterile water for 1 minute each time, and the surface moisture was blotted with sterile paper to obtain 50 sterilized explants;

[0045] 2. Induction of callus: the detoxified explants were cut into 2.0cm long segments and inoculated in callus induction medium MS+6-BA 1.5mg / L +NAA0.3 mg / L for induction , control the culture temperature at 23°C, first cultivate in the dark for 24h, then carry out normal light culture, the light time is 10h / day, and the light i...

Embodiment 2

[0052]A kind of tissue culture method taking Pan's Bingdengyulu inflorescence axis as explants, it comprises the steps:

[0053] 1. Selection and disinfection of explants: In mid-April, the inflorescence rachis of Yulu in full flowering stage was selected as explants. The disinfection method was to rinse with running water for 30 minutes, then disinfect with 75% ethanol for 30 seconds on a sterile operating table, and then use 0.1 % mercuric chloride for 8 minutes, and finally washed 6 times with sterile water, each time for 1 minute, and blotted the surface moisture with sterile paper to obtain 50 sterilized explants;

[0054] 2. Induction of callus: the detoxified explants were cut into 2.0cm long segments and inoculated in callus induction medium MS+6-BA 1.5mg / L +NAA0.3 mg / L for induction , control the culture temperature at 27°C, first cultivate in the dark for 24h, then carry out normal light culture, the light time is 14h / day, and the light intensity is 1800lx. After 35 ...

Embodiment 3

[0061] After the explants were sterilized, they were inoculated on the induction medium containing different hormone ratios, and the effects of different medium on the callus induction rate were compared. See Table 1 for details.

[0062] Table 1 Effect of induction medium on callus induction rate

[0063]

[0064] It can be seen from Table 1 that on the MS+6-BA 1.5 mg / L +NAA0.3 mg / L medium, the explants developed best, and the callus induction rate was the highest, which was 100%.

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Abstract

The invention belongs to the technical field of plant tissue culture, and provides a tissue culture method using Pan's Bingdeng Yulu inflorescence rachis as explants, comprising the following steps: selecting healthy Yulu inflorescence rachis, cleaning and disinfecting, and obtaining aseptic explants Inoculating the aseptic explant into a callus-inducing medium to induce callus after cutting into sections; then cutting the callus into small pieces and transferring it to an adventitious bud differentiation medium for differentiation and cultivation to obtain clustered buds; Then, after cultivating the strong seedlings of the ramets of clustered buds, they are transferred to the rooting medium for cultivation to obtain complete plants with roots, and finally the seedlings are hardened and cultivated. The present invention obtains Yulu tissue culture seedlings by in vitro culture of Yulu inflorescence rachis tissue, the method is easy to obtain materials, is convenient for disinfection, has high callus differentiation rate, high rooting rate, ensures strain advantage, and can rapidly and massively reproduce a large number of excellent plants. Seedlings of Bingdeng Yulu.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and relates to a tissue culture method of the succulent plant Pan's Bingdeng Yulu, in particular to a tissue culture method using the inflorescence axis of the Pan's Bingdeng Yulu as an explant. Background technique [0002] Yulu ( Haworthia obtusa var. pilifera ) is a "soft leaf" variety in the genus Liliaceae. The plants are exquisite and small, with rich varieties, and the leaf color is crystal clear and full of changes. It is like a living handicraft and is very cute. It is one of the more popular small succulent plant varieties in recent years. More and more favored by succulent plant lovers. Pan's Bingdeng Yulu is one of the varieties with more outstanding ornamental value among Yulu varieties. Most of them are propagated by division and leaf cuttings. However, these methods have a low reproductive rate, the propagation method damages the growth of the mother plant, and th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 束晓春李乃伟王忠张凤姣王涛吴宝成
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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