58 results about "Helleborus thibetanus" patented technology

The invention discloses a Helleborus thibetanus extract, a separation method of effective components in the Helleborus thibetanus extract and compounds obtained by the separation method. The Helleborus thibetanus extract is obtained by the following steps: respectively extracting a Helleborus thibetanus medicinal material by the use of 95% ethanol and 50% ethanol which account for 6-8 times the weight of the Helleborus thibetanus medicinal material for 2-4 times, merging extracts, condensing and drying, adding water for dispersion, successively carrying out extraction by the use of petroleum ether and ethyl acetate, condensing an ethyl acetate extract, and drying. It is found through an experimental research that the ethyl acetate extraction part in the ethanol extract of the Helleborus thibetanus extract has good pain-suppressing and anti-inflammatory effects, and the ethyl acetate extraction part can be used in the preparation of an anti-inflammatory and pain-suppressing medicine; and 17 compounds are obtained by separation of the ethyl acetate extraction part, wherein two compounds are new compounds and 12 compounds are firstly obtained by separation of Helleborus thibetanus. The invention lays a foundation for establishing content determination items of the Helleborus thibetanus medicinal material and a medicinal preparation containing Helleborus thibetanus, is beneficial to raising quality standard and quality controllability of the Helleborus thibetanus medicinal material and lays a foundation for further development and utilization of Helleborus thibetanus.

The invention relates to new application of total saponins of helleborus thibetanus franch, in particular to the new application of the total saponins of the helleborus thibetanus franch in protecting the liver function. The total saponins of the helleborus thibetanus franch is studied to find that the total saponins of the helleborus thibetanus franch has the protection function on mouse acute liver injury models prepared from carbon tetrachloride, and then is used for preventing and treating the liver injuries caused by many reasons.

The invention relates to a rapid breeding method based on tissue culture for helleborus thibetanus. The method comprises the following steps: (1) preparing a bud inducing culture medium, an adventitious bud proliferation culture medium, a seedling strengthening culture medium, and a rooting culture medium; and (2) performing tissue culture on the helleborus thibetanus. According to the method provided by the invention, through a tissue culture technology, the annual propagation coefficient is improved to 1:20000 and the cost is reduced by 0.8-1.0 yuan / plant compared with those of the prior art; and the propagation speed and seedling uniformity are greatly improved through tissue culture seedlings of the helleborus thibetanus, and original female parent characters are better maintained.

The present invention relates to helleborus thibetanus medicinal liquor for effectively curing the diseases of rheumalgia, traumatic injuries, rheumatic arthritis, rheumatic arthralgia and myalgia and arthralgia due to wind-cold-dampness, etc. Said medicinal liquor is made up by using Chinese medicinal materials of helleborus thibetanus, shibazheng, randia spinosa, pangxueteng, rabdosia rubescens and white liquor through a certain preparation process.

The invention discloses a beta cardiac glycoside and a preparation method and application thereof. The structure of the beta cardiac glycoside is as follows: the compound is extracted from Helleborus thibetanus (Ranunculaceae): dry powder at the rhizome part of the Helleborus thibetanus is soaked with 95% ethanol, and then is concentrated under reduced pressure to obtain a concentrated solution; the concentrated solution is respectively extracted by using ethyl acetate and n-butyl alcohol which are equal in volume; column chromatography separation is peformed on an n-butanol extraction phase by adopting a D101 macroporous resin column, a normal-phase silica gel column, a reverse-phase ODS (Octadecylsilyl), a gel column and the like, and finally purifying is performed by adopting semi-preparative high performance liquid chromatography. The beta cardiac glycoside has a good inhibition effect on angiogenesis of human umbilical vein endothelial cells (HUVEC) so that the beta cardiac glycoside can be prepared into a novel anti-angiogenesis medicine. The invention provides a material basis for novel anti-vascular drugs, and is beneficial to further development of medicinal value and clinical application of helleborus thibetanus.

The invention discloses a pharmaceutical preparation for treating asthma and a preparing method, and belongs to the field of traditional Chinese medicine. The pharmaceutical preparation comprises stachys sieboldi miq, ruddle, fourleaf peperomia herbs, mountain ash, herbs of ceylon leucas, asarum maximum, seeds of zanthoxylom bungeanum maxim, lemon, hibiscus syriacus, india mustard seeds, herbs of common evolvulus, coffee senna seeds, herbs of cuneate lespedeza, helleborus thibetanus, ginkgo seeds and marsdenia longipes. The selected medicinal materials of the pharmaceutical preparation are suitable in compatibility, the traditional Chinese medicine theory and the modern medicine theory are met, and the pharmaceutical preparation has the effects of clearing heat, removing toxicity, relieving coughing and asthma, clearing the lung and the liver, astringing the lung and relieving asthma, and is convenient to use, good in absorption effect and free of poor, toxic and side effects; according to clinical verification, the pharmaceutical preparation has the quite good relieving effect and the quite good treating effect on treating asthma, and is suitable for clinic treating and nursing applying and popularizing of asthma.

The invention discloses a traditional Chinese medicinal preparation for treating Henoch-Schonlein purpura nephritis. The disease belongs to hematuria, edema, purple plague and other fields in traditional Chinese medicine. The disease is caused by pathogenic heat accumulation in body, affection by pathogenic wind or improper diet, or contact of foreign matter, resulting in fighting of wind heat and moist heat or intense toxic heat, burn of blood vessels and damage of kidney. Traditional Chinese medicinal materials with effects of clearing away heat and toxic materials, removing heat from blood to stop bleeding, discharging heat, dispelling blood stasis and nourishing liver and kidney, including Blumea lacera, Hedyotis hispida, Cephalanoplos setosum, Arnebia saxatilis, Helleborus thibetanus, Wikstroemia indica, Herba Echiptae, Osmanthus heterophyllus and Herba Lespedezae Cuneatae, are decocted in water to obtain decoction. Through clinical trials, the total effective rate is 96.7%, no adverse effect appears during medication, and no recurrence appears in 19 follow-up patients in 3 years.

The invention discloses a Helleborus thibetanus extract, a separation method of effective components in the Helleborus thibetanus extract and compounds obtained by the separation method. The Helleborus thibetanus extract is obtained by the following steps: respectively extracting a Helleborus thibetanus medicinal material by the use of 95% ethanol and 50% ethanol which account for 6-8 times the weight of the Helleborus thibetanus medicinal material for 2-4 times, merging extracts, condensing and drying, adding water for dispersion, successively carrying out extraction by the use of petroleum ether and ethyl acetate, condensing an ethyl acetate extract, and drying. It is found through an experimental research that the ethyl acetate extraction part in the ethanol extract of the Helleborus thibetanus extract has good pain-suppressing and anti-inflammatory effects, and the ethyl acetate extraction part can be used in the preparation of an anti-inflammatory and pain-suppressing medicine; and 17 compounds are obtained by separation of the ethyl acetate extraction part, wherein two compounds are new compounds and 12 compounds are firstly obtained by separation of Helleborus thibetanus. The invention lays a foundation for establishing content determination items of the Helleborus thibetanus medicinal material and a medicinal preparation containing Helleborus thibetanus, is beneficial to raising quality standard and quality controllability of the Helleborus thibetanus medicinal material and lays a foundation for further development and utilization of Helleborus thibetanus.

The invention discloses an external application lotion for treating gynecological cervical erosion and gynecological inflammations, and a preparation method thereof. The external application lotion is prepared from the following raw materials, wherein the raw materials comprise, by weight, 40-60 parts of aconitum kusnezoffii, 20-30 parts of helleborus thibetanus franch, 20-30 parts of periploca forrestii schltr, 30-50 parts of poiret barberry root, 20-40 parts of spinyleaf pricklyash root, 30-50 parts of mahonia bealei, 20-30 parts of borneol, 0-10 parts of eucommia, 0-10 parts of honeysuckle, 0-10 parts of akabia stem and 0-10 parts of cinnamomum camphora. A plurality of natural plant drugs with effects of anti-inflammatory, detoxication, kidney-yin tonifying, congestion dissipating, impurity discharging, body resistance supporting and basis consolidating, are screened through the formula of the external application lotion. The external application lotion belongs to the filed of external application drugs, therefore the external application lotion provided by the present invention does not have any side effect. According to theoretical formulations inherited from traditional Miaomedicine, the external application lotion provided by the present invention can provide a strong therapeutic effect for the gynecological cervical erosion and the gynecological inflammations. In addition, the external application lotion does not contain antibiotics and has no stimulation to human body.

The invention relates to the field of traditional Chinese medicines, in particular to a traditional Chinese medicine capsule for treating lymphoma and a preparation method thereof. The traditional Chinese medicine capsules are prepared by the following traditional Chinese medicine raw materials according to the parts by weight: 15-20 parts of Prunella vulgaris, 10-15 parts of seaweed, 10-15 parts of xanthophyll seed, 20-25 parts of Salvia miltiorrhiza, 8-10 parts of blood dried flower, Chuanxiong 8-10 parts, 15-20 parts of curcuma, 8-10 parts of ground beetle, 8-10 parts of centipede, 10-15 parts of red peony, 15-20 parts of turtle shell, 25-30 parts of Hedyotis diffusa, Ebony vine 35-40 parts, iron chopsticks 35-40 parts, astragalus 8-10 parts, heterophylla 10-15 parts, Atractylodes macrocephala 10-15 parts, jujube 6-8 parts, angelica 8-10 parts, raw land 8-10 parts, 8-10 parts of Ophiopogon japonicus, 8-10 parts of wolfberry, 15-20 parts of donkey-hide gelatin. The traditional Chinese medicine of the present invention has the effects of clearing away heat and detoxification, promoting blood circulation and removing blood stasis, has remarkable curative effect on lymphoma, can improve the immune function of the human body, inhibit the generation and spread of cancer cells, and play the role of antibacterial and anti-inflammatory, toxin and pus drainage, and stagnation and swelling reduction. The medicinal material is easy to obtain, the cost is low, the cure rate is high, there is no recurrence after the cure, it is safe and reliable, and it is worthy of widespread clinical application.

The invention relates to a traditional Chinese medicine for treating alveolar hypoventilation syndrome, which is prepared from the following medical materials in weight ratio: 12 parts of helleborus thibetanus, 8 parts of caesalpinia cucullata, 10 parts of antelope horns, 12 parts of sea horses, 14 parts of common andrographis herb, 10 parts of crocus sativus, 8 parts of radix scrophulariae, 9 parts of salvia chinensis, 12 parts of blister beetles, 14 parts of cortex lycii radicis, 12 parts of butterflybush flower, 10 parts of rehmannia glutinosa libosch, 14 parts of moutan barks and 12 parts of rhizome anemarrhenae. Proved by clinical experiments, the traditional Chinese medicine can be used for safely and effectively treating alveolar hypoventilation syndrome.

The invention discloses equipment for detecting effective components of Chinese herbal medicine helleborus thibetanus. The equipment comprises an extracting tank; a drive motor is arranged at the topof the extracting tank, and a rotating shaft of the drive motor is connected with the top of a drive shaft through a flexible transmission structure; a connecting shaft sleeve is sheathed at the bottom of the outer wall of the drive shaft, stirring rods are uniformly arranged on each of the left side and the right side of the outer wall of the connecting shaft sleeve, a feeding tube and a samplingtube are respectively arranged on the left side wall of the extracting tank, an extracting liquid feeding tube and an extracting liquid discharging tube are respectively arranged on the right side wall of the extracting tank, and a waste discharge tube is arranged at the bottom of the extracting tube. The equipment disclosed by the invention has the beneficial effects that the structural design optimization is reasonable; the sampling detection can be performed in the extracting process of the Chinese herbal medicine helleborus thibetanus; extracting liquid is purified and extracted again according to sampling detection results, so that the extracting period is reduced, and the production speed is improved; meanwhile, the extracting process is simplified, the production efficiency is improved, the production cost is reduced, and the operation is convenient.

The invention provides a composition with an effect of inhibiting activity of tumor cells and application of the composition. The composition comprises the following components: natural capsaicin, diosgenin, a hymenocallis littoralis extract, a helleborus thibetanus volatile oil extract, a saussurea obvallata extract and an ervatamia hainanensis tsiang extract. The composition is obtained from natural medicinal plants, the antitumor active ingredients extracted and separated from the natural plants have characteristics of numerous quantity, diversified structure types, remarkable drug effect and the like, and characteristics of diversified antitumor activity and multi-target action are an antitumor drug research and development thought and a potential strategy.

The invention discloses a traditional Chinese medicine preparation for nursing diabetes and a preparation method thereof, and belongs to the field of traditional Chinese medicines. The traditional Chinese medicine preparation disclosed by the invention comprises the following crude drugs: semen pharbitidis, corn stigma, astragalus membranaceus, cyclocarya paliurus, pyrrosia lingua, coffee senna, herba aristolochiae, pine needle, rhizoma smilacis glabrae, gardenia, helleborus thibetanus, flatstem milkvetch seed, water hyacinth, soletellina diphos, folium ilicis purpurea and running mallow root. The traditional Chinese medicine preparation disclosed by the invention is appropriate in compatibility of selected medicinal materials, accords with the theories of the traditional Chinese medicine and pharmacy and the modern medicine and pharmacology, has the effects of inducing diuresis to reduce edema, tonifying the liver and the kidneys, calming the liver and benefitting bile, can be used for repairing pancreas damaged tissue, has a significant treatment effect on various symptoms caused by the diabetes, is safe and reliable, free of toxic and side effects, low in price, strange in effect and practical, and worthy of extensive popularization and application in clinic, and is used for treating both symptoms and root causes.

The invention provides a tissue culture propagation method for helleborus thibetanus. The tissue culture propagation method comprises the following steps of (1), obtaining and treating an explant; (2), carrying out adventitious bud induction culture; (3), carrying out adventitious bud subculture multiplication culture; (4), rooting culture; and (5), hardening seedlings and transplanting to obtain helleborus thibetanus seedlings. According to the method, hormone and a culture environment are comprehensively adjusted, so that the proliferation multiple of the adventitious buds of the helleborus thibetanus reaches 3.5 or above, the rooting rate reaches 90% or above, and the problems that the helleborus thibetanus is low in propagation coefficient, difficult to root and long in production period are solved; and besides, by keeping the excellent characters of the excellent strains, seedlings with stable excellent characters can be produced, standardization and factory operation are easy, and the excellent seedlings with the unified standard are provided for the market.

The invention provides a cold-curing preparation and a detection method for an intermediate product. The cold-curing preparation is prepared from Geum japonicum Thunb, Helleborus thibetanus, Folium Hibisci Mutabilis, purple perilla, fewflower lysionotus herb, herba kalimeridis, Schizonepeta, and auxiliary materials. Compared with the prior art, on the basis of current cold-curing preparation quality control, a method for measuring the content of gallic acid and lysionotin of an intermediate product and a preparation is added to the detection method. The detection method is accurate; the sensitivity is high; the repeatability is good; the detection result is stable; and the quality of the cold-curing preparation can be effectively controlled.

The invention discloses a preparation for treating acute urethritis. The preparation is prepared from the following main raw materials in parts by weight: 6-10 parts of caulis akebiae, 9-14 parts of miquel maqzus, 10-15 parts of nauclea officinalis, 8-16 parts of glossogyne tenuifolia, 9-15 parts of cherry leaves, 11-16 parts of lotus petiole petiolus, 7-14 parts of fructus kochiae, 9-13 parts of helleborus thibetanus, 8-14 parts of colysis digitata, 7-13 parts of obscured homalomena rhizome, 11-17 parts of lophatherum gracile, 9-16 parts of Japanese nanocnide herb, 7-15 parts of fortune microsorium herb with root, 6-14 parts of pogostemon auricularius and 8-15 parts of bambooleaf pricklyash root. The preparation is prepared from compatible medicinal materials, accords with traditional Chinese medicine and modern medicine theories, has the effects of clearing heat, removing toxicity, nourishing yin, reducing internal fire, diminishing swelling, relieving pain, inducing diuresisand dredging stranguria, has the advantages of remarkable curative effect, quick response and relapse prevention after healing when being used for treating urethritis, and is safe and reliable.

The present invention discloses a helleborus thibetanus Qufeng Tongluo Zhitong medicine for effectively curing the diseases of rheumatic arthralgia, rheumatic arthritis, rheumatoid arthritis, traumatic injury and solf tissue contusion, etc. with obvious therapeutic effect. It is made up by using 14 Chinese medicinal materials of helleborus thibetanus, aliteng, asarum sieboldii, peperomia terraphylla, lamiophlomis rotata and others and adding ethyl alcohol through a certain preparation process.

The invention discloses ointment for orthopedics department. The ointment is prepared from the following raw materials: 9 to 12 parts of pseudodrynaria coronans, 7 to 10 parts of semen impatientis, 4 to 6 parts of spica prunellae, 12 to 14 parts of radix notoginseng, 9 to 13 parts of radix alangii, 5 to 6 parts of radix angelicae lateralis preparata, 8 to 9 parts of sangusis draconis, 10 to 12 parts of myrrh, 6 to 8 parts of helleborus thibetanus, 4 to 5 parts of camphor, 5 to 7 parts of saffron, 9 to 11 parts of radix dipsaci, 6 to 9 parts of frankincense and 5 to 8 parts of radix et rhizoma rhei. The ointment disclosed by the invention can be used for rapidly improving microcirculation so that pain and numbness are rapidly eliminated and a fibrous ring of an intervertebral disk is rapidly repaired, and furthermore, the effect of obtaining immediate result is realized; the ointment has rapid and good curative effect and no side effect; the ointment for the orthopedics department, which is reasonably blended, has the advantages that the raw materials are convenient to take and a preparation process is simple, convenient and easy; the treatment cost of patients is low and the curative effect is rapid.

The invention discloses an oral medicine for treating enteritis and dysentery. The oral medicine comprises the following traditional Chinese medicine materials in parts by weight: 5-15 parts of fimbriate calanthe pseudobulb, 10-20 parts of helleborus thibetanus, 10-20 parts of herb of diversifolious pimpinella, 10-20 parts of shortleaf kyllinga herb, 10-15 parts of scorzonera albicaulis bunge, 5-10 parts of tricyrtis macropoda and 5-10 parts of viola arcuata. The medicine is prepared from pure traditional Chinese medicines, so that the traditional Chinese medicine is free of toxic or side effect, free of drug resistance and has the advantages of quick acting, short treatment course, low recurrence rate and high safety and reliability when used for treating enteritis and dysentery.

The invention relates to the technical field of traditional Chinese medicines and particularly relates to a traditional Chinese medicine for curing cystolith. The traditional Chinese medicine for curing cystolith comprises the following raw materials: dandelion, akebiaquinata, codonopsis pilosula, thunder god vine, helleborus thibetanus franch, cynoglossum officinale, rhizoma cyperi, fruit of manchurian catalpa, poria cocos, sri lanka pouzolzia herb, crotalaria tetragona roxb, yam rhizome, polygala tenuifolia, corydalis tuber, radix achyranthis bidentatae, pericarpium citri reticulatae, veronicastrum sibiricum, pyrrosia lingua, hemsleya macrosperma, medical stones, lysimachia christinae hance, flos hibisci and sanmon oil. The medicinal materials adopted in the invention complement one another, are prepared according to the theory of Chinese medical recipe and certain weight proportion, and play a remarkable effect on curing cystolith, the traditional Chinese medicine for curing cystolith is safe and reliable, can reach nidus directly, has the effects of promoting blood flow and dispersing swelling, clearing away heat and toxic materials, dissolving and breaking stones, inducing diuresis to reduce edema and the like, can treat both symptoms and root causes, has no toxic or side effect, can discharge stones quickly, and ensures no recurrence.

The present invention provides a migraine treating wine medicine, which adopts radix anemones rivularis, siegesbeckia orientalis l, herba seu radix clintonae udensis, crocus sativus l, ligusticum chuanxiong hort, angelica dahurica, asarum sieboldii miq, pawpaw, cassia twig, magnolia officinalis, common schizomussaenda rhizome or leaf, corydalis decumbens, cynanchum paniculatum, mulberry root, cocculus laurifolius, japanese pagodatree bark, hazelnut or is added with sansevieria trifasciata prain, slender-spike gouania stem and leaf, reineckia carnea, triangular-toothed shield herb, zhuyelian, flower of javan waterdropwort, helleborus thibetanus franch, datura seed, petasites japonicas, rubus chingii and tithymalus-like pedilanthus herb as the main raw materials. According to the present invention, the clinical treatment effect of the migraine treating spray is observed; the method comprises that the migraine treating spray is externally coated on 32 cases in the treatment group and the control group comprising 32 cases orally take Toutongling capsules; the results show that in the treatment group 29 cases are cured, the 2 cases have the effect, the 1 case does not have the effect, and the total effective rate is 96.87% while in the control group 20 cases are cured, the 4 cases have the effect, the 8 cases do not have the effect, and the total effective rate is 75%, and with the statistic treatment, the significant difference (p is less than 0.05) exists in the treatment effects of the two groups; and the conclusion is that the migraine treatment effect of the migraine treating spray is significant.

The invention discloses a swelling eliminating and itch relieving ointment for treating chilblain. The ointment is prepared from the following raw materials in parts by weight: 8 to 13 parts of eggplant root, 6 to 12 parts of radermachera sinica, 4 to 9 parts of dominant pulicaria flower, 7 to 11 parts of euonymus bungeanus, 5 to 10 parts of bearded mallotus leaf, 3 to 8 parts of hovenia acerba leaf, 6 to 11 parts of alligator weed, 4 to 7 parts of tetrapanax papyriferus, 8 to 11 parts of longstamen loosestrife herb, 6 to 13 parts of Chinaberry bark, 8 to 12 parts of actinidia eriantha leaf, 6 to 10 parts of helleborus thibetanus, 4 to 11 parts of garcinia multiflora, 7 to 12 parts of pteris semipinnata, and 5 to 11 parts of impatiens leptocaulon. The formula is scientific, the traditional Chinese herbals generate a synergetic effect, and the ointment has the functions of dissipating stasis, eliminating swelling, regulating qi, and activating blood, and has the advantages of good curative effect, relapse prevention, and rapid effect.

The invention provides a traditional Chinese medicine for treating liver-kidney yin deficiency type hemicrania and a preparation method for the traditional Chinese medicine. The traditional Chinese medicine is prepared from the following raw material medicines: root of common triplostegia, herba aristolochiae, entada phaseoloides, helleborus thibetanus, wall fern, herb of threeflower bluebeard, fructus cinnamomi, concha arcae, root of tartary buckwheat, silene baccifera, root of womwoodleaf mmpelocissus, psychotria serpens, china root greenbrier leaves and uraria crinita. The traditional Chinese medicine is definite in treating effect on the liver-kidney yin deficiency type hemicrania and low in toxic and side effect.

The present invention discloses a Qushi Zhentong medicine liquor for effectively curing the diseases of rheumatoid arthritis, rheumatic numbness, chronic arthralgia, rheumatic arthralgia and myalgia, soft tissue injury and traumatic injury, etc. with obvious therapeutic effect. It is made up by using 5 Chinese medicinal materials of coral ardisia root, helleborus thibetanus, rabdosia rubescens, impatiens balsamina root, glechoma hederacea and white liquor through a certain preparation process. Its therapeutic effect is reliable, and its cure rate is above 90%.

The invention provides an extraction method of a plant composition containing herba epimedii. The plant composition is prepared from 100 parts of herba epimedii, 40 parts of galium verum, 60 parts of helleborus thibetanus and 50 parts of rubus pluribracteatus roots by adopting the microwave extraction method, therefore, the content is greatly increased, and the using amount is decreased. The invention further provides an application of the plant composition containing the herba epimedii in preparation of medicine for inhibiting proliferation of melanic rattus lung fibroblast NRL2.

The invention provides a traditional Chinese medicine for treating deep second-degree burns by combining traditional Chinese medicine with western medicine and a preparation method. The traditional Chinese medicine is prepared from runcinate knotweed herb, brooklet anemine roots, herba desmodii caudati, wintersweet roots or stems, roots of helleborus thibetanus Franch., oldworld arrowhead leaves, white loosestrife herb, giantleaf ardisia leaves, bigleaf fig roots, fiddleleaf fig roots or leaves, suffruticulose jussiaea herb, loureiro clematis herb, Tibet lancea herb, common epipremnum herb, leaves of sisal hemp-plant and all-grass of dysophylla benthamiana Hance. The traditional Chinese medicine for treating the deep second-degree burns has the advantages that the traditional Chinese medicine cooperates with a conventional method of the western medicine for treating the deep second-degree burns, the treatment effect is good, toxic and side effects and adverse reactions do not exist, the second-degree burns can be cured within a shorter time, and the pain of a patient is reduced.

The invention relates to the technical field of tissue culture of helleborus thibetanus, in particular to a breeding method of helleborus thibetanus seedlings, which specifically comprises the following steps: S1, selection and treatment of explants; s2, induction of sterile test-tube plantlets; s3, proliferation culture of test-tube plantlets; the method is easy to operate, the culture medium is convenient to prepare, explant materials are easy to obtain, the inductivity, the multiplication coefficient and the rooting rate are high, regenerated plants reserve the excellent characters of female parents, plant regeneration of the tissue culture seedlings of the helleborus thibetanus is achieved, and the method is an effective way for breeding excellent varieties of the helleborus thibetanus.

The invention relates to the field of traditional Chinese medicine formula, and specifically relates to a traditional Chinese medicine composition for treating urinary tract infection. The traditional Chinese medicine composition is characterized by being composed of the following raw materials in percentage by weight: 3 to 12% of rabdosia longituba, 3 to 15% of polygonum aviculare, 6 to 10% of helleborus thibetanus, 3 to 10% of raspberry, 3 to 10% of radix berberidis, 3 to 10% of iris tectorum, 3 to 10% of folium isatidis, 5 to 10% of felwort, 5 to 20% of bighead atractylodes rhizome, 5 to 15% of hydrocharis dubia, 10 to 15% of radix aconiti lateralis preparata, 10 to 15% of pyrrosia lingua, 10 to 30% of aeginetia indica, 6 to 10% of semen lepidii, 10 to 30% of day lily, 6 to 10% of fiveleaf akebia fruit, and 2 to 10% of dayflower. The raw materials mentioned above are soaked in water, and then decocted. The decoction is filtered to remove dregs so as to obtain filtrate. The composition has the functions of clearing heat, removing toxin, excreting dampness, promoting urination, eliminating inflammation, relieving pain, boosting qi, engendering liquid, reconciling superficies and interior, dispelling wind, treating fever, and activating blood, improves the in-vivo and vi-vitro environment, and treats or relieves various symptoms of urinary tract infection.

The invention discloses a traditional Chinese medicine composition and preparation for treating rheumatoid arthritis and a preparing method and application thereof. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 80-100 parts of helleborus thibetanus, 20-30 parts of schisandra chinensis, 20-30 parts of ganoderma lucidum, 15-30 parts ofradix aucklandiae, 25-50 parts of vervain, 20-30 parts of radix paeoniae rubra, 30-40 parts of nduoh nyaatv buerng, 50-80 parts of a traditional Chinese medicine named 'Sanheliu' according to the Chinese pronunciation, 20-40 parts of polygonum multiflorum, and 30-60 parts of camphor root. The composition has good therapeutic effect on rheumatoid arthritis, and has the curative effect superior to that of tripterygium glycoside.