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Method for breeding helleborus thibetanus seedlings

The technology of iron chopsticks and seedlings is applied in the field of breeding of iron chopsticks seedlings, which can solve the problem of less tissue of iron chopsticks, and achieve the effects of high rooting rate, high value-added coefficient and quality assurance.

Pending Publication Date: 2022-07-12
SHANGHAI INST OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few research reports on the tissue culture of iron chopsticks

Method used

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  • Method for breeding helleborus thibetanus seedlings
  • Method for breeding helleborus thibetanus seedlings

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Embodiment 1

[0031] A method for breeding iron chopsticks seedlings, which specifically comprises the following steps:

[0032] S1. Selection and treatment of explants: In the growing season, select plants that are free from diseases and insect pests and grow well as explants, remove leaves, pretreat, sterilize with sterilants, immerse in sterile water, and then excise part of the tissue at the wound. , cut the stem into segments, each segment with a bud point;

[0033] S2. Induction of sterile test tube seedlings;

[0034] S3, the proliferation culture of test tube seedlings;

[0035] S4. Rooting culture of test tube seedlings.

[0036] Wherein, the specific steps in the step S2 are: inoculating the explants treated in the step S1 in the induction medium, cultivating in the dark for 10 days, then transferring to the light culture, and obtaining sterile test tube seedlings after 45 days.

[0037] Wherein, the step S3 is specifically: cutting the explant obtained in step S2 into segments...

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Abstract

The invention relates to the technical field of tissue culture of helleborus thibetanus, in particular to a breeding method of helleborus thibetanus seedlings, which specifically comprises the following steps: S1, selection and treatment of explants; s2, induction of sterile test-tube plantlets; s3, proliferation culture of test-tube plantlets; the method is easy to operate, the culture medium is convenient to prepare, explant materials are easy to obtain, the inductivity, the multiplication coefficient and the rooting rate are high, regenerated plants reserve the excellent characters of female parents, plant regeneration of the tissue culture seedlings of the helleborus thibetanus is achieved, and the method is an effective way for breeding excellent varieties of the helleborus thibetanus.

Description

technical field [0001] The invention relates to the technical field of tissue culture of iron chopsticks, in particular to a breeding method of iron chopsticks seedlings. Background technique [0002] Helleborus is a plant of the family Ranunculaceae and the genus Helleborus. It is a perennial evergreen herb with a low plant type, peculiar flowers and leaves. The flowering period is from winter to the next spring. Very popular as a garden ground cover and flower arrangement. There are 22 species in the genus, most of which are distributed in the Balkans of Europe. Only Helleborusthibetanus is distributed in East Asia and is endemic to China. It has wild distribution in Sichuan, Shaanxi, Gansu and other places. Its roots and rhizomes are used medicinally, which are cool in nature and bitter in taste, and have the functions of clearing away heat and detoxifying, reducing swelling and pain, promoting blood circulation and dissipating blood stasis. The hellebore aglycone in th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/002A01H4/008Y02P60/21
Inventor 孔家烨董竞宇张志国王艺程叶常青
Owner SHANGHAI INST OF TECH
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