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Tissue culture rapid propagation method of Guangdong anoectochilus roxburghii

A technology of tissue culture rapid propagation and clematis, which is applied in the field of plant tissue culture, can solve problems such as difficulty in forming commercial scale planting, low reproduction coefficient, immature embryos, etc., solve technical problems and bottlenecks, and facilitate unified management , the effect of increasing the number of seedlings

Inactive Publication Date: 2012-12-26
SOUTH CHINA AGRI UNIV
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

But the embryo of the seed of golden clematis is immature, not easy to germinate; the propagation coefficient of cutting method is low, it is difficult to form commercial scale planting

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The material used is wild Cantonese clematis, which is taken from Nankun Mountain in Guangdong.

[0024] Follow the steps below to propagate Cantonese clematis:

[0025] 1. Take a wild Cantonese clematis plant and cut it into small sections each containing an axillary bud.

[0026] 2. Put the stem section into a 0.1% mercuric chloride solution with a mass-volume ratio for disinfection for 10 minutes, and wash it with sterile water three times for 2 minutes each time.

[0027] 3. Inoculate the sterilized stem segments into the induction medium on the ultra-clean workbench, the composition of the induction medium is MS+1.0mg / l 6-BA+0.1mg / l IBA, and the pH value is 5.8. Artificial light with an intensity of 2000Lx and a culture temperature of 25°C.

[0028] 4. One axillary bud can induce about 2 to 4 new buds. After 2 to 4 adventitious buds are induced, the buds are transferred to the same medium (composed of MS+1.0mg / l 6-BA+0.1mg / l IBA, pH 5.8) for subculture and proli...

Embodiment 2

[0034] The material used is wild Cantonese clematis, which is taken from Nankun Mountain in Guangdong.

[0035] Follow the steps below to propagate Cantonese clematis:

[0036] 1. Take a wild Cantonese clematis plant, and cut off a small section containing a terminal bud.

[0037] 2. Put the stem section into a 0.1% mercuric chloride solution to sterilize for 10 minutes, and wash it with sterile water three times, each time for 2 minutes.

[0038] 3. Inoculate the sterilized stem segments into the induction medium on the ultra-clean workbench, the composition of the induction medium is MS+1.0mg / l 6-BA+0.1mg / l IBA, and the pH value is 5.8. Artificial light with an intensity of 2000Lx and a culture temperature of 25°C.

[0039] 4. One axillary bud can induce about 2 to 4 new buds. After the adventitious buds were induced, the buds were transferred to the same medium (composed of MS+1.0 mg / l 6-BA+0.1 mg / l IBA, pH value 5.8) for subculture and multiplication. After 2 months, t...

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Abstract

The invention discloses a tissue culture rapid propagation method of Guangdong anoectochilus roxburghii, which selects an axillary bud or a terminal bud of anoectochilus roxburghii in the Guangdong area in China as an explant and obtains a mass of vegetative propagated progeny plants by inducing the adventitious buds, carrying out proliferation and carrying out rooting cultivation. By adopting the method of the invention, one axillary bud can be induced to generate about 2 to 4 sprouts, after two months, the proliferation coefficient is between 3 and 4, and the rooting rate can reach over 90 percent. Therefore, the method greatly improves the seedling production capacity of Guangdong anoectochilus roxburghii, shortens the seedling culture time, saves the planting cost and provides the technical guarantee for the storage of the genetic resources of Guangdong anoectochilus roxburghii.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for tissue culture and rapid propagation of Aureus aureus in Guangdong. Background technique [0002] Anoectochitus formosanus (Anoectochitus formosanus) is a traditional precious medicinal material in my country. It belongs to the perennial herb of the family Orchidaceae. Golden clematis can also be used as an ornamental plant with high ornamental value. Auranthus clematis is rich in vitamins and polysaccharides, with high content of trace elements and amino acids, and has antibacterial, anticancer, cardiotonic, analgesic, and hypoglycemic effects. Due to the good efficacy of clematis in the treatment of liver disease, hypertension, diabetes and tumors, it has increasingly attracted the attention of the medical community. [0003] But the clematis seeds are tiny, the germination rate is low, its growth has strict requirements on the ecological...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 任顺祥王泽清
Owner SOUTH CHINA AGRI UNIV
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