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Anti-browning and tissue culture proliferation method for dendrobenthamia tonkinensis

A four-photographing flower and anti-browning agent technology, applied in the field of plant tissue culture, can solve the problems of long seedling cultivation cycle, low production efficiency, low reproduction coefficient, etc., and achieve the effect of fast growth, normal color and good growth

Active Publication Date: 2017-12-22
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a stable and efficient method for anti-browning and tissue culture propagation of Tokyo Sizhaohua to solve the problems of long seedling cultivation period, low reproduction coefficient and low production efficiency in the traditional Tokyo Sizhaohua propagation method , by optimizing the operation of various stages such as material collection, disinfection, establishment of a sterile anti-browning system, induction of adventitious buds, proliferation and cultivation of Tokyo Sizhaohua, the anti-browning ability of adventitious buds of Tokyo Sizhaohua is effectively improved, and the promotion of Tokyo Sizhaohua Produce a large number of adventitious buds with the excellent characteristics of the parents in a short period of time, laying the foundation for the establishment of the rapid propagation system of Tokyo Sizhaohua in the later period

Method used

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  • Anti-browning and tissue culture proliferation method for dendrobenthamia tonkinensis
  • Anti-browning and tissue culture proliferation method for dendrobenthamia tonkinensis
  • Anti-browning and tissue culture proliferation method for dendrobenthamia tonkinensis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1, pollution anti-browning comparison

[0030] Selection of explants: In mid-April, May, July, August, October, and November 2014, healthy and disease-free vegetative branches of the current year were collected in Tokyo Sizhao Flower Nursery, Baima Base, Nanjing Forestry University. Cut out the stem segment with buds about 3-4 cm long at the node, keep 1 axillary bud, put it in the ice box and bring it back to the laboratory for later use.

[0031] Sterilization of explants: trim the leaves on the stems, keep the petioles and 1 / 5 of the leaves, rinse with tap water for 1 to 2 hours, then disinfect the cleaned stems on the ultra-clean workbench, first use 70 Soak in % alcohol for 30s, rinse with sterile water for 2 to 3 times, then soak in 0.1% mercuric chloride for 5, 7, 9, and 11 minutes, rinse with sterile water for 4 to 5 times, and set aside (see Table 1 below).

[0032] Establishment of a sterile anti-browning system: put the sterilized flower stems of To...

Embodiment 2

[0044] Embodiment 2, aseptic system germination rate comparison

[0045] Selection of explants: In mid-April 2015, healthy and disease-free vegetative branches of the current year were collected in Tokyo Sizhao Flower Nursery, Baima Base, Nanjing Forestry University, and 3-4 cm long strips were cut from the top 1 and 2 nodes. For the bud stem segment, keep 1 axillary bud, put it in the ice box and bring it back to the laboratory for future use.

[0046] Sterilization of explants: trim the leaves on the stems, keep the petioles and 1 / 5 of the leaves, rinse with tap water for 1 to 2 hours, then disinfect the cleaned stems on the ultra-clean workbench, first use 70 Soak in % alcohol for 30 seconds, rinse with sterile water for 2 to 3 times, then soak in 0.1% mercury chloride for 7 minutes, rinse with sterile water for 4 to 5 times, and set aside.

[0047] Establishment of a sterile anti-browning system: put the sterilized flower stems of Tokyo Sizhao on a sterilized filter paper...

Embodiment 3

[0051] Embodiment 3, proliferation culture formula comparison

[0052] Selection of explants: In mid-April, May, July, August, October, and November 2014, healthy and disease-free vegetative branches of the current year were collected in Tokyo Sizhao Flower Nursery, Baima Base, Nanjing Forestry University. Cut out the stem segment with buds about 3-4 cm long at the node, keep 1 axillary bud, put it in the ice box and bring it back to the laboratory for later use.

[0053] Sterilization of explants: trim the leaves on the stems, keep the petioles and 1 / 5 of the leaves, rinse with tap water for 1 to 2 hours, then disinfect the cleaned stems on the ultra-clean workbench, first use 70 Soak in % alcohol for 30s, rinse with sterile water for 2 to 3 times, then soak in 0.1% mercuric chloride for 5, 7, 9, and 11 minutes, rinse with sterile water for 4 to 5 times, and set aside.

[0054] Establishment of a sterile anti-browning system: put the sterilized flower stems of Tokyo Sizhao o...

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Abstract

The invention discloses a stable and efficient anti-browning and tissue culture proliferation method for dendrobenthamia tonkinensis. The anti-browning and tissue culture proliferation method comprises the following steps: 1) taking materials: taking current-year nutritional branches which have no diseases and grow healthy in sunny days from April to May in spring; 2) sterilizing, wherein a sterilization method comprises the step of sterilizing with alcohol for 30s and sterilizing with 0.1 percent mercuric chloride for 7min; 3) establishing a sterile anti-browning system: flatly laying disinfected stem sections on disinfected filtering paper and airing; inoculating the stem sections with a culture medium containing a 200mg.L<-1> PVP (Polyvinyl Pyrrolidone) anti-browning agent; after sprouts grow to 3cm to 4cm, carrying out proliferation culture; (4) carrying out the proliferation culture: inoculating the induced sprouts with a proliferation culture medium (including WPM (Woody Plant Medium), 1.0mg.L-16-BA (Benzylaminopurine) and 0.05mg.L-1NAA (1-Naphthylacetic Acetic Acid)) and carrying out subculture once every other 20 to 25 days. According to the anti-browning and tissue culture proliferation method for the dendrobenthamia tonkinensis, various phases of tissue culture proliferation of the dendrobenthamia tonkinensis are optimized, the browning rate is effectively reduced to 17.87 percent and the proliferation coefficient is improved to reach 3.09.

Description

technical field [0001] The invention relates to a method for anti-browning and tissue culture multiplication of Tokyo Sizhaohua, which belongs to the technical field of plant tissue culture. Background technique [0002] Tokyo Sizhaohua (Dendrobenthamia tonkinensis) belongs to the plant of the genus Dendrobenthamia (Cornaceae) in the family Cornaceae, and is an evergreen small tree or shrub. Its tree shape is beautiful, its branches are flat and well-defined (Hatta et al, 1999). It can be seen in bright leaves in spring, jade flowers in summer, and red leaves and red fruits in autumn. It is widely used in landscaping, park landscaping, and potted art; Wounds can reduce swelling, roots and seeds can be decocted and taken in water to enrich blood, which has certain medicinal value; its fruit contains high fat and high calcium, can be eaten fresh, made into vinegar and wine, and is a wild fruit with nutritional value (Han Weidong, 1993; Vareed, 2005); its wood is hard, straigh...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张洋洑香香鲁强尚旭岚
Owner NANJING FORESTRY UNIV
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