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Tissue culture and rapid propagation culture medium for gerbera jamesonii and culture method thereof

A technology of tissue culture rapid propagation and cultivation method, which is applied in the field of gerbera tissue culture rapid propagation medium and its cultivation, can solve the problems affecting the survival rate of transplanted seedlings, etc., and achieve the improvement of flower yield and quality, high survival rate, The effect of improving quality

Active Publication Date: 2015-07-01
LINYI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the roots are elongated, they are intertwined in the culture medium, adding steps such as washing seedlings and cleaning the root system to the transplanting of gerbera tissue culture seedlings, which will affect the survival rate of transplanted seedlings

Method used

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  • Tissue culture and rapid propagation culture medium for gerbera jamesonii and culture method thereof
  • Tissue culture and rapid propagation culture medium for gerbera jamesonii and culture method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. The configuration of culture medium

[0026] The revised MS medium is based on the MS medium, and the contents of thiamine hydrochloride, pyridoxine hydrochloride, nicotinic acid and calcium pantothenate are adjusted to 10mg / L and 1.0mg / L respectively under the condition of other components being unchanged. , 1.0mg / L, 1.0mg / L, and mix thoroughly to form a mixed solution.

[0027] The induction medium is a revised MS medium supplemented with 3.0mg / L 6-BA+0.5mg / L IAA+1.0mg / L CPPU (N-2-chloro-4-pyridylphenyl-N'-phenylurea), 30g / L sugar, 0.8% agar, adjusted to pH 5.8 with 0.1N NaOH or HCl, autoclaved at 104kPa, 121°C for 20min.

[0028] Proliferation medium is revised MS medium+0.6mg / L 6-BA+0.2mg / L NAA+60mg / L sulfate adenine.

[0029] The rooting medium was revised MS+0.2mg / L NAA.

[0030] 2. Gerbera chrysanthemum tissue culture rapid propagation method

[0031] 1) Disinfection of explants

[0032] Wash the young tender receptacle with a diameter of less than 0.5 cm...

Embodiment 2

[0041] Utilize the embodiment 1 scheme to carry out tissue culture rapid propagation of Gerbera chrysanthemum, and record and count the data of its cultivation process.

[0042] 1) Induction of adventitious buds

[0043] When the florets were cultured on the adventitious bud induction medium for one week, the cut part swelled, and the florets on the receptacle swelled and turned green after about 7 days of cultivation, and the cut surface of the torus was swollen, but there was no callus. After cultivating for about 10 days, callus tissue began to appear on the floret, the floret swelled into a chrysanthemum shape, and small buds began to differentiate on the callus tissue. When cultured for 35-42 days, the florets gradually turned black, and the calluses of the blackened florets continuously differentiated into clumps of small buds. The adventitious bud differentiation ability of different varieties is different. The adventitious bud differentiation rate of Daxueta chrysanth...

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Abstract

The invention discloses a tissue culture and rapid propagation culture medium for gerbera jamesonii and a culture method thereof. The tissue culture medium comprises an induction medium, a proliferation medium and a rooting medium. Tender receptacles of gerbera jamesonii are regenerated in vitro by using the culture medium. The technology comprises the processes of induction, proliferation, rooting, acclimatizating and transplanting and the like of an adventitious bud. By adopting the method and formula, not only is the proliferation coefficient of gerbera jamesonii improved and high quality strong seedlings cultured, but also the culture period can be shortened and the survival rate can be improved. Moreover, the gerbera jamesonii further blossoms in advance in about 15 days, the differentiation time of plantlets in plastic tunnel culture can be shortened in advanced and the flower-picking yield and quality can be improved.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a gerbera tissue culture rapid propagation medium and a cultivation method thereof. Background technique [0002] Gerbera jamesonii Bolus (Gerbera jamesonii Bolus), also known as Gerbera, is a perennial herb of Compositae (Compos itae), Gerbera Bolus (Gerbera Bolus), plant height 30-40cm, leaves mostly basal, petiole length 10-20cm, It has huge flowers, tall and straight flower branches, bright colors, long flowering period, high flower yield, convenient cultivation and management, and can supply cut flowers year round under warm conditions. Therefore, its production scale is getting larger and larger, and it is one of the five largest cut flowers in the world. Has high commercial value. Gerbera is a cross-pollinated plant. Under natural conditions, the seed setting rate is very low and the seeds are prone to lose germination ability. In addition, there is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 廉玉姬
Owner LINYI UNIVERSITY
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