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Method for breeding wenshan paphiopedilum seedlings by using somatic embryo

A technology of somatic embryos and immature embryos, which is applied in the field of breeding P. wenshanensis seedlings, can solve the problems of effective reproduction bottleneck, difficulty in protection and development, and low reproduction coefficient of P. wenshanensis, so as to promote callus growth and increase value-added Efficiency and value-added time, and the effect of improving reproductive efficiency

Active Publication Date: 2013-09-11
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Propagation by traditional ramets has a low reproduction coefficient, and it is difficult to meet the requirements of protection and development.
Propagation of Paphiopedilum plants using traditional orchid aseptic sowing techniques is not only difficult, but also difficult to reproduce, which creates a major bottleneck for the effective reproduction of Paphiopedilum wenshanensis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Seed germination: In May when Paphiopedilum wenshanensis blooms, 5 days after flowering, select healthy and disease-free plants for pollination and self-fertilization at 11:00 in the morning without rain, and stop 1 day before self-fertilization and 1 day after self-fertilization Carry out water spraying to plant; 150 days after pollination fruit is ripe, select full fruit as ripe Paphiopedilum arvenides fruit, carry out following sterilization to ripe Paphiopedilum arvenides fruit: be 70% alcohol wiping fruit surface with volume ratio concentration, then Wash 3 times with sterile water, sterilize with 0.1% mercuric chloride for 10 minutes, wash 3 times with sterile water, then soak with 10% sodium hypochlorite for 10 minutes, and finally wash 3 times with sterile water, Cut the fruit and take out the seeds, inoculate in the following germination induction medium: Ms + 2.0mg / L 6-BA + 100ml / L coconut milk + 80g / L fresh banana mash + 50g / L mashed potatoes + 5g / L activa...

Embodiment 2

[0024](1) Seed germination: In May when Paphiopedilum wenshanensis blooms, 6 days after flowering, select healthy and disease-free plants for pollination and self-fertilization at 10 am without rain. The 1 day before self-fertilization and 1 day after self-fertilization are not correct. The plant is sprayed with water; 150 days after pollination, the fruit is ripe, and the plump fruit is selected as the mature Paphiopedilum arvenides fruit, and the ripe Paphiopedilum arvensis fruit is sterilized, and the fruit surface is wiped with alcohol with a volume ratio concentration of 70%, and then successively use Wash 3 times with sterile water, sterilize with 0.1% mercuric chloride for 10 minutes, wash 3 times with sterile water, soak with sodium hypochlorite with 10% concentration for 10 minutes, wash 3 times with sterile water, cut Open the fruit, take out the seeds, and inoculate the following germination induction medium: Ms+2.0mg / L 6-BA+100ml / L coconut milk+80g / L fresh banana ma...

Embodiment 3

[0030] (1) Seed germination: During the flowering season of P. wenshanensis in May, 7 days after flowering, select healthy and disease-free plants for pollination and self-fertilization at 2 am without rain, 1 day before self-fertilization and 1 day after self-fertilization Do not spray the plants with water; the fruit is ripe 150 days after pollination, select the plump fruit as the mature Paphiopedilum arvenides fruit, sterilize the ripe Paphiopedilum arvenides fruit, wipe the surface of the fruit with alcohol with a concentration of 70% by volume, and then Wash 3 times with sterile water, sterilize with 0.1% mercuric chloride for 10 minutes, wash 3 times with sterile water, soak with 10% sodium hypochlorite for 10 minutes, and finally wash 3 times with sterile water. Cut the fruit and take out the seeds, inoculate in the following germination induction medium: Ms + 2.0mg / L 6-BA + 100ml / L coconut milk + 80g / L fresh banana mash + 50g / L mashed potatoes + 5g / L activated carbon +...

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Abstract

The invention provides a method for breeding wenshan paphiopedilum seedlings by using somatic embryo. After mature wenshan paphiopedilum fruits are sterilized, the fruits are cut apart to obtain seeds, the seeds are inoculated in an induced germination medium to obtain young embryo, and the young embryo is inoculated in a liquid nutrient medium to obtain the somatic embryo; differentiation seedlings are obtained through differentiation cultivation; the differentiation seedlings are inoculated in a root medium to perform root cultivation, and finally the seedlings are exercised and transplanted, namely the wenshan paphiopedilum seedlings are obtained. The method for breeding wenshan paphiopedilum seedlings by using the somatic embryo obtains the young embryo after sterile germination of the wenshan paphiopedilum mature fruits, breaks dormancy of shoots, promotes germination of the seeds and growth of callus, performs rapid cultivation on the plant somatic embryo, enables wenshan paphiopedilum tissue cultivation enhancement factor to be improved to above 5 times from original 1 time, and greatly improves reproduction efficiency of the wenshan paphiopedilum. Survival rate of the wenshan paphiopedilum seedlings after transplanting reaches above 90%.

Description

technical field [0001] The invention relates to a method for breeding Paphiopedilum arvensis seedlings by using somatic embryos, and belongs to the technical field of plant tissue culture. Background technique [0002] Wenshan Paphiopedilum ( Paph. Wenshanense ) for Orchidaceae (Orchidaceae) Paphiopedilum (Paphiopedium ) plant is a treasure in Orchidaceae, listed as IV-level rare and endangered species by the "Washington Convention", and is a world-class flower name with great ornamental value. It has a long flowering period, solemn and elegant flower color, and unique flower shape. Its lips are shaped like slippers, so it is called "slipper orchid". The back calyx is particularly developed and has gorgeous patterns. Although China is rich in Paphiopedilum species, due to over-harvesting, rampant smuggling out of the country, and habitat destruction, the number of wild Paphiopedilum has decreased sharply in the past decade, and its distribution area has gradually shrunk. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 李涵蒋亚莲蔡艳飞陈敏唐路瑶王继华瞿素萍张颢
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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