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Tissue culture rapid breeding method of Chinese medicine abrotanum

A tissue culture, Artemisia annua technology, applied in the field of plant reproduction, can solve the problems of restricting the popularization and use of artemisinin drugs and high market price of artemisinin, and achieve the effects of stable content, easy material acquisition and high value-added coefficient.

Inactive Publication Date: 2008-05-21
INST OF BOTANY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These problems lead to the high market price of artemisinin, which seriously limits the popularization and use of artemisinin drugs in poor and backward malaria-infested areas. Content

Method used

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  • Tissue culture rapid breeding method of Chinese medicine abrotanum
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1, Tissue Culture Rapid Propagation of Chinese Medicine Artemisia annua

[0020] 1. Preparation and sterilization of culture medium

[0021] Primary medium: MS+6-BA 0.5mg / L+GA 1.0mg / L.

[0022] Proliferation medium: MS+6-BA 1.5mg / L+NAA 0.2mg / L.

[0023] Rooting medium: MS+IBA 0.5mg / L.

[0024] The above medium was sterilized at 121.3°C for 20 minutes.

[0025] 2. Primary culture

[0026] Take the terminal bud or axillary bud of the plant of the traditional Chinese medicine Artemisia annua L., soak it with 70% (volume percentage) alcohol for 1 minute, and then put it into 0.1% (mass percentage) mercury chloride solution for sterilization After 5-10 minutes, after rinsing with sterile water for 3 times, the terminal buds or axillary buds were transferred into a triangular flask (capacity 250ml) equipped with primary medium, and the number of explants in each triangular flask was 5. The culture was carried out under the following culture conditions: 25° C., li...

Embodiment 2

[0043] Example 2, Tissue Culture Rapid Propagation of Chinese Medicine Artemisia annua

[0044] 1. Preparation and sterilization of culture medium

[0045] Primary medium: MS+6-BA 0.1mg / L+GA 0.2mg / L.

[0046] Proliferation medium: MS+6-BA 0.5mg / L+NAA 0.1mg / L.

[0047] Rooting medium: MS+IBA 0.2mg / L.

[0048] The above medium was sterilized at 121.3°C for 20 minutes.

[0049] 2. Primary culture

[0050] Take the terminal bud or axillary bud of the traditional Chinese medicine Artemisia annua L., soak it with 70% (volume percentage) alcohol for 1 minute, and then put it into 0.1% (mass percentage) mercuric chloride solution to sterilize for 5 -10 minutes, after rinsing with sterile water for 3 times, the terminal buds or axillary buds were transferred to a triangular flask (capacity 250ml) equipped with the primary culture medium, and the number of explants in each triangular flask was 5. Cultured under the following culture conditions:

[0051] 22°C, 14 hours of light and...

Embodiment 3

[0062] Example 3, Tissue Culture Rapid Propagation of Chinese Medicine Artemisia annua

[0063] 1. Preparation and sterilization of culture medium

[0064] Primary medium: MS+6-BA 2.0mg / L+GA 2.0mg / L.

[0065] Proliferation medium: MS+6-BA 2.0mg / L+NAA 0.5mg / L.

[0066] Rooting medium: MS+IBA 1.0mg / L.

[0067] The above medium was sterilized at 121.3°C for 20 minutes.

[0068] 2. Primary culture

[0069] Take the terminal bud or axillary bud of the traditional Chinese medicine Artemisia annua L., soak it with 70% (volume percentage) alcohol for 1 minute, rinse it with sterile water for 3 times, and then add 0.1% (mass percentage) mercuric chloride solution for 5-10 minutes, after rinsing with sterile water for 3 times, transfer the terminal buds or axillary buds into a triangular flask (capacity 250ml) equipped with primary culture medium, the number of explants in each triangular flask for 5. Cultured under the following culture conditions:

[0070] 30°C, 16 hours of lig...

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Abstract

The invention discloses a tissue culture rapid propagation method of the traditional Chinese medicine Artemisia annua. The tissue culture rapid propagation method of the traditional Chinese medicine Artemisia annua is to cultivate the terminal buds or axillary buds of the traditional Chinese medicine Artemisia annua in the primary culture medium to obtain sterile seedlings; the primary culture medium is to add 6-BA and GA to the MS medium In the obtained culture medium, the final concentration of the 6-BA is 0.1-2.0 mg / liter, and the final concentration of the GA is 0.2-2.0 mg / liter. The method of the present invention has the following advantages: easy to obtain materials, very easy to obtain aseptic materials, low variation rate, high value-added coefficient, 4-week multiplication multiple can reach 5 to 6 times, the rooting rate of tissue culture seedlings reaches more than 93%, and the transplanting survival rate Up to 95%, with a strong factory production capacity. By using the method of the invention to breed high-yielding Chinese herb Artemisia annua strains, the high artemisinin content of offspring can be stabilized, thereby increasing the artemisinin output.

Description

technical field [0001] The invention relates to a plant propagation method, in particular to a tissue culture rapid propagation method of the traditional Chinese medicine Artemisia annua L. Background technique [0002] Artemisinin, an effective antimalarial monomer isolated from the traditional Chinese medicine Artemisia annua L., is the first drug independently invented by Chinese scientists and recognized globally. The anti-malarial drugs developed based on artemisinin are highly valued by the World Health Organization (WHO). Malaria is the most widespread tropical parasitic infectious disease with the highest morbidity and mortality in the world today. According to the statistics report of the World Health Organization in recent years: there are currently 2.9 billion people living in malaria-endemic areas in the world, with an annual incidence of 300-500 million and a death toll of 2-3 million. Malaria has become the number one killer in Africa. In order to control the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N5/04
Inventor 叶和春李国凤王红颜芳刘本叶
Owner INST OF BOTANY CHINESE ACAD OF SCI
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