Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for inducing grape fruit callus and its culture medium

A technique for inducing medium and callus, applied in the field of plant biology, to achieve the effect of strong proliferation ability, high value-added coefficient and high consistency

Active Publication Date: 2019-11-12
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Only studying the callus of grape leaves cannot meet the requirements of scientific research, especially for grape fruit, the fruit itself must be studied in order to achieve the purpose of producing high-quality fruit

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for inducing grape fruit callus and its culture medium
  • A method for inducing grape fruit callus and its culture medium
  • A method for inducing grape fruit callus and its culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Test materials

[0027] The plant material used is the fruit of the ‘Summer Black’ grape 10-18 days, 20-25 days, and 35-40 days after flowering.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an induction method for a grape fruit callus and a culture medium thereof. The culture medium comprises the composition: a basal culture medium, 0.8-1.2 mg / L of TDZ, 0.3-0.5 mg / L of IBA, 18-22 g / L of sugar, and 5-7 g / L of agar. The induction method is characterized by comprising the steps: sterilizing grape fruits, then peeling, taking pulp blocks, inoculating the inductionculture medium with the pulp blocks, and carrying out induction culture until the callus is formed. The culture method of the fruit callus of 'Xiahei' grapes is successfully established, and the induction rate of the fruit callus reaches 100%, a new way is provided for molecular biology and genetic engineering research of the 'Xiahei' grapes, a new experimental material is provided for the genetic engineering research and gene transformation of the grapes, and meanwhile, a reference is also provided for culture of the grape fruit callus.

Description

Technical field [0001] The invention relates to the field of plant biotechnology, in particular to a method for culturing grape fruit pulp callus. Background technique [0002] At present, the research of grape genome is a hot spot in grape science. The research direction of grape genome mainly involves plant resistance, fruit quality and fruit production characteristics. Among them, the important economic characteristics of grapes are especially the molecules related to the formation of fruit quality. Mechanism research has also become one of the key issues in grape genome research. The ultimate goal of molecular mechanism research is to verify gene function. However, because the transgenic technology of grapes is not mature enough, the efficiency of gene transformation is extremely low, and the transgenic cycle is long and time-consuming. Callus is a good material for verifying gene function. Callus refers to a group of non-polar and vigorously dividing parenchymal cells forme...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
CPCA01H4/001A01H4/008
Inventor 董慧嵇怡杨淑莉孙颖王凌飞丁勇民张嘉芯唐雨诗
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com