Determination method for freshness of fresh cordyceps sinensis
A technology for fresh Cordyceps sinensis and Cordyceps sinensis is applied in the field of detection of raw materials of traditional Chinese medicinal materials, which can solve the problems of poor reproducibility of the detection of Cordyceps sinensis freshness, inability to accurately reflect the freshness of Cordyceps sinensis, and high experimental cost, and achieve easy learning and operation, dyeing and extraction time. The effect of shortening and low experimental cost
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Embodiment 1
[0040] To investigate the effects of different pretreatments on the freshness of fresh Cordyceps sinensis, the specific steps are as follows:
[0041] (1) Preparation of the test solution:
[0042] a. The same batch of 6 fresh Cordyceps sinensis stored at -5°C to -10°C for about 2 months was taken out from the storage environment and divided into two groups (5% glucose treatment group and room temperature treatment group), each group 3 roots. The 5% glucose treatment group was placed in a 5% glucose solution preheated to 37°C and treated in a 37°C water bath for 15 minutes, and the room temperature treatment group was placed at room temperature for 15 minutes, and then the body parts of 6 fresh Cordyceps sinensis were placed on the 4th- 7. Uniformly slice the foot section, with a thickness of 1-2mm;
[0043] b. Take 0.2g of 6 fresh Cordyceps slices in step a respectively, and every 3 same treatments are 1 group, totally 2 groups, and place them in 0.3% TTC solution (with pH ...
Embodiment 2
[0063] To investigate the influence of different parts of fresh Cordyceps sinensis on the determination of freshness, the specific steps are as follows:
[0064] (1) Preparation of the test solution:
[0065] a. Take the same batch of 3 fresh Cordyceps sinensis stored at -5°C to -10°C for about 2 months from the storage environment, place them in a 5% glucose solution preheated to 37°C and treat them in a 37°C water bath for 15 minutes, then Divide 3 fresh Cordyceps sinensis into 4 sections (fresh Cordyceps sinensis section, worm body head to 4th pair of feet section, worm body head 4-7 pair of foot sections, worm body head The seventh pair of feet to the tail section) is uniformly sliced, with a thickness of 1-2mm;
[0066] b. Take 0.2g of 4 different section sections of 3 fresh Cordyceps sinensis in step a respectively, and every 3 same sections form a group, totally 4 groups, and place them respectively in a 0.3% TTC solution with a mass fraction of 0.3% (pH 7.3-7.5 phosp...
Embodiment 3
[0079] The impact of different dyeing times on the freshness of fresh Cordyceps sinensis, the specific steps are as follows:
[0080] (1) Preparation of the test solution:
[0081] a. The same batch of 15 fresh Cordyceps sinensis stored at -5°C to -10°C for about 1 week was taken out from the storage environment, placed in a 5% glucose solution preheated to 37°C for 15 minutes in a 37°C water bath, and then 15 pieces of fresh Cordyceps sinensis worms from the head to the 4th to 7th pair of legs are sliced evenly, with a thickness of 1-2mm;
[0082] b. Take 0.2g of 15 fresh Cordyceps slices in step a respectively, and every 3 pieces are 1 group, totally 5 groups, and place them respectively in 0.3% TTC solution (dissolved with phosphate buffer solution of pH 7.3-7.5) prepared) in 15 beakers at 37°C in a constant temperature water bath and protected from light for 0.5h, 1h, 2h, 4h, and 8h, respectively. After the staining time was 0.5h, 1h, 2h, 4h, and 8h, the fresh Cordyceps...
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