The invention discloses a quick
hormone-free cultivation method of
dendrobium huoshanense seedlings. The method comprises the steps as follows:
dendrobium huoshanense capsules which grow about 100 days and are not fully matured are picked in Huoshan, and ivory powdered young embryos in the capsules are taken as explants; a novel optimized culture medium is adopted, and compared with a conventional MS culture medium, dosage of chemicals of inorganic salt and the like as well as
sugar is smaller, edible substances or waste materials such as mashed yam,
bran paste, banana
skin homogenate and the like are added in the culture medium respectively at different cultivation stages, a
carbon source adopts commercially available edible
sugar, and
hormone is not added; seedlings are healthy and strong after cultivation for six months, each
seedling is 3-4 cm high and has 3-4 sections, 6-8 leaves and 4-5 roots, the
root length is 2-4 cm, and the transplant
survival rate is higher than 95%; and later, the seedlings are transferred to a
natural environment of an original area for cultivation, so that wild
dendrobium nobile quality can be maintained. With the adoption of the quick
hormone-free cultivation method of the
dendrobium huoshanense seedlings, the young
embryo breeding rate is high, the
seedling breeding period is shortened, the culture medium cost is low, the utilization values of yams, brans, banana
skin (waste materials) are improved, and the optimized culture medium is suitable for large-scale industrial production.