Quick hormone-free cultivation method of dendrobium huoshanense seedlings
A technology of Dendrobium huoshanensis and a cultivation method, applied in the field of plant biology, can solve the problems of high cost of seedlings, long reproduction cycle, influence on the quality of Dendrobium huoshan, and achieve the effects of maintaining quality, low cost of culture medium, and shortening breeding cycle of seedlings
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment Construction
[0044] A hormone-free rapid culture method for Dendrobium huoshanense seedlings, comprising the following steps:
[0045] (1) Protocorm culture
[0046] ① Selection of explants
[0047] Huoshan Dendrobium fruit pods that have grown for about 100 days and have not yet fully matured are picked from Huoshan, soaked in 1% detergent for 10 minutes, and then scrubbed with running water;
[0048] ②Sterilization
[0049] Put the washed fruit pods into a sterile beaker on an ultra-clean workbench, soak them in 75% alcohol for 30 seconds, rinse them once with sterile water, then soak them with 5% sodium hypochlorite solution for 20 minutes, and rinse them twice with sterile water.
[0050] ③ Inoculation and culture
[0051] Strictly aseptic operation, the scalpel is cut longitudinally along the fruit pod, and the milky white powdery immature embryos are directly inoculated on No. 1 medium: nutrient element mother solution 100ml / L + potato puree 100g...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com