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Miniature Chinese rose tissue culture breeding method

A rose and miniature technology, applied in the field of plant cultivation, can solve the problems of high cost of cutting propagation, low survival rate, difficulty in mass production, etc., and achieve the effect of increasing the reproduction rate and survival rate

Active Publication Date: 2015-02-25
启东欣荣旅游开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Miniature roses can be propagated by seeds, cuttings and tissue culture techniques. Cuttings are often used for propagation, but the cost of cutting propagation is high, the survival rate is low, and it is difficult to mass-produce, while the tissue culture technology has high reproduction efficiency, short cycle, and genetic shape. Stable, easy to manage, suitable for industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Select robust stem segments of miniature roses that have been sterilized in advance as explants, and after decontamination, sterilization and disinfection, cut the explants into stem segments containing 2 complete scales, and immerse the stem segments in the culture medium, wherein KT content of 0.2mg / L, 6-BA content of 0.5mg / L, NAA content of 0.005mg / L, pH value of 5, adding 0.3% activated carbon, adjusting the culture temperature to 25°C, 1500Lux intensity of light for 10h, induction Stem segments with axillary buds.

[0024] Cut off the axillary buds and immerse them in the medium, in which the content of KT is 0.2mg / L, the content of 6-BA is 2mg / L, the content of NAA is 0.2mg / L, the pH value is 6, and the cultivation temperature is adjusted to 27°C and the intensity is 1500Lux Under the light for 10 hours, until the axillary buds grow to 2-3cm, normal seedlings are formed, and the seedlings are immersed in the rooting medium, in which the content of 1 / 2MS+IBA is 0.5...

Embodiment 2

[0028] Select robust stem segments of miniature roses that have been sterilized in advance as explants, and after decontamination, sterilization and disinfection, cut the explants into stem segments containing a complete scale leaf, and immerse the stem segments in the culture medium, wherein KT The content of 6-BA is 0.2mg / L, the content of 6-BA is 2mg / L, the content of NAA is 0.005mg / L, the pH value is 6, add 0.5% activated carbon, adjust the culture temperature to 25℃, and light at 1200Lux for 15h to induce stem Sectional axillary buds.

[0029] Cut off the axillary buds and immerse them in the culture medium, in which the content of KT is 0.2mg / L, the content of 6-BA is 0.5mg / L, the content of NAA is 0.005mg / L, the pH value is 5, and the culture temperature is adjusted to 30℃, 1200Lux Under the intensity of light for 15 hours, until the axillary buds grow to 2-3cm, normal seedlings are formed, and the seedlings are immersed in the rooting medium, in which the content of 1 / ...

Embodiment 3

[0033] Select robust stem segments of miniature roses that have been sterilized in advance as explants, and after decontamination, sterilization and disinfection, cut the explants into stem segments containing 2 complete scales, and immerse the stem segments in the culture medium, wherein KT The content of 6-BA is 0.2mg / L, the content of 6-BA is 1mg / L, the content of NAA is 0.01mg / L, the pH value is 5.6, add 0.4% activated carbon, adjust the culture temperature to 26°C, and light at 1300Lux for 12h to induce stem Sectional axillary buds.

[0034] Cut off the axillary buds and immerse them in the culture medium, in which the content of KT is 0.2mg / L, the content of 6-BA is 0.1mg / L, the content of NAA is 0.1mg / L, the pH value is 6, and the culture temperature is adjusted to 28℃, 1300Lux Intensity of light for 12 hours, until the axillary buds grow to 2-3cm, normal seedlings are generated, the seedlings are immersed in the rooting medium, wherein the content of 1 / 2MS+IBA is 0.6mg...

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PUM

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Abstract

The invention discloses a miniature Chinese rose tissue culture breeding method. The miniature Chinese rose tissue culture breeding method is characterized by carefully selecting stem section explants and formula of an optimized culture medium, and combining technologies such as illumination to obtain a miniature Chinese rose with high survival rate. The miniature Chinese rose tissue culture breeding method specifically comprises the following steps: firstly taking stem sections with complete scale leaves selected from strong miniature Chinese roses as explants, then immersing the explants in the culture medium, irradiating by sunlight, generating axillary buds, taking down the axillary buds and immersing the axillary buds into a root growing culture medium, combining the illumination, transferring seedlings into a potted tray after the axillary buds grow to seedlings which are 2-3cm long, cooperating with technologies of coating films, regularly irradiating and sterilizing to obtain a tissue culture miniature Chinese rose. The miniature Chinese rose prepared by the method is high in survival rate and high in growth coefficient, and is suitable for industrialized mass production.

Description

Technical field: [0001] The invention belongs to the technical field of plant cultivation methods, in particular to a propagation method of micro rose tissue culture. Background technique: [0002] Rose is one of the top ten famous flowers in my country, also known as Yueyuehong, Yueyuekai, periwinkle, Four Seasons Rose, etc. It is an evergreen or semi-evergreen shrub belonging to the Rosaceae family. Rose has the advantages of rich color and pattern, long flowering period, and strong adaptability. Therefore, there are many varieties of roses, which are divided into fresh-cut roses, miniature roses, tree roses, strong-flowered roses, shrub roses, and ground cover roses. With the progress of society, the development of science and technology and the improvement of people's appreciation level, there are more and more varieties and colors in the flower season, and double colors, skin colors, and striped twisted colors are also becoming more and more common. [0003] Miniature ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陶建平
Owner 启东欣荣旅游开发有限公司
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