Fermentation preparation method of eurotium cristatum melanin
A coronoid saccharomyces and melanin technology, applied in biochemical equipment and methods, fermentation, chemical instruments and methods, etc., can solve the problems of expensive tyrosinase, unfavorable factory production, complex synthesis process, etc., and achieve Shorten the extraction time, increase the yield, the effect of high yield
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[0015] Put the coronoid loose capsule strain into sterilized 400 ml liquid medium (1L culture bottle), the formula of the liquid medium is: 3g of sodium nitrate, 1g of dipotassium hydrogen phosphate, 0.5g of magnesium sulfate, potassium chloride 0.5g, ferrous sulfate 0.01g, sucrose 30g, distilled water 1L, pH 5.5, inoculum size 1%. Put it into a full-temperature shaker for cultivation, the cultivation condition is 28°C, 180r / min, when the cultivation reaches 5 days, a large number of hyphae balls of S. coronis will be produced in the culture bottle. Place the silk balls in a 4°C refrigerator and let them stand for 8-10 hours, then continue to put them in a full-temperature shaker at 28°C, 180r / min for cultivation, and a large amount of melanin molecules will be observed to be secreted into the medium the next day. The base began to turn dark brown, and when the culture reached the 10th day, the culture was terminated, and the melanin was ready to be harvested.
[0016] After ...
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