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Microwave extraction method for spirulina polysaccharide

A technology of spirulina polysaccharide and extraction method, which is applied in the field of polysaccharide preparation, can solve the problems of energy consumption, time consumption, and low yield, and achieve the effects of high extraction efficiency, reasonable process, and ideal yield

Inactive Publication Date: 2016-02-24
RONGCHENG GUANGRUN SEAFOOD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, hot water method, cold alkali method, etc. are mostly used for the extraction of spirulina polysaccharides. These methods all have disadvantages such as time-consuming, energy-consuming, and low yield.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A microwave extraction method of spirulina polysaccharide, which is through the following process steps:

[0019] (1) Microwave extraction: Weigh spirulina powder and add distilled water with a material-liquid ratio of 1:20, use an ultrasonic processor with a rated power of 150W, and use an ultrasonic treatment time of 3 minutes, then use intermittent microwave extraction to carry out microwave extraction; the extract is sent to In the centrifuge, the speed of the centrifuge is controlled at 5000r / min, and the centrifugation time is 12min. The supernatant is taken to obtain the crude extract of spirulina polysaccharide; among them, the intermittent microwave extraction method is to control the microwave power to 500W, and the microwave radiation time of each cycle is 20S. Time 3min, repeated 6 cycles;

[0020] (2) TCA method for protein removal: add an equal volume of trichloroacetic acid solution with a concentration of 8% to the crude extract of spirulina polysacchari...

Embodiment 2

[0024] A microwave extraction method of spirulina polysaccharide, which is through the following process steps:

[0025] (1) Microwave extraction: Weigh spirulina powder and add distilled water with a material-liquid ratio of 1:10. The rated power of the ultrasonic processor is 100W, and the ultrasonic treatment time is 5min. Among them, control the speed of the centrifuge to 4000r / min, centrifuge time 15min, take the supernatant, and obtain the crude extract of spirulina polysaccharide; wherein, the intermittent microwave extraction method is to control the microwave power to 450W, the microwave radiation time of each cycle is 25S, and the intermittent time is 2min , repeated for 7 cycles;

[0026] (2) TCA method for protein removal: Add an equal volume of trichloroacetic acid solution with a concentration of 5% to the crude extract of spirulina polysaccharide obtained in step (1), shake for 15 minutes; stand still for 4 hours, centrifuge, and take the supernatant solution; ...

Embodiment 3

[0031] A microwave extraction method of spirulina polysaccharide, which is through the following process steps:

[0032] (1) Microwave extraction: Weigh spirulina powder and add distilled water with a material-liquid ratio of 1:30, the rated power of the ultrasonic processor is 200W, and the ultrasonic treatment time is 2min; then microwave extraction is carried out by intermittent microwave extraction; the extract is sent into a centrifuge Among them, control the speed of the centrifuge at 6000r / min, centrifuge for 10min, take the supernatant, and obtain the crude extract of spirulina polysaccharide; among them, the intermittent microwave extraction method is to control the microwave power to 570W, the microwave radiation time of each cycle is 15S, and the intermittent time is 15min , repeated for 5 cycles;

[0033] (2) TCA method for protein removal: add an equal volume of trichloroacetic acid solution with a concentration of 10% to the crude extract of spirulina polysacchar...

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Abstract

The invention discloses a microwave extraction method for spirulina polysaccharide. The method mainly overcomes the problems of great consumption of time and energy and low yield in extraction of spirulina polysaccharide with conventional hot water methods and cold alkali methods The microwave extraction method comprises the following steps: adding spirulina powder into distilled water according to a material-liquid ratio, carrying out ultrasonic treatment, then carrying out microwave extraction by using an intermittent microwave extraction method and then carrying out centrifugation so as to obtain crude spirulina polysaccharide extract; adding the crude spirulina polysaccharide extract into a trichloroacetic acid solution to remove protein; and then adding active carbon TS4 for decoloring and carrying out vacuum freeze drying so as to obtain the spirulina polysaccharide. The microwave extraction method has the advantages of reasonable extraction process, mild polysaccharide extraction conditions, no destroy to effective components, high extraction efficiency, thorough removal of impurities, ideal yield and suitability for large-scale factory production.

Description

technical field [0001] The invention relates to the preparation of polysaccharides, in particular to a microwave extraction method of spirulina polysaccharides. Background technique [0002] We know that Spirulina (Spirulina) is a kind of low-level plant, belonging to Cyanophyta, Oscillatoriaceae, because of its balanced and comprehensive nutrition, it is popular all over the world and has become the best health maintenance food. Spirulina polysaccharide is an extremely important physiologically active ingredient in spirulina. It has unique effects in anti-tumor, anti-virus, hypoglycemic, anti-aging, anti-radiation, anti-coagulation and improving immunity. It has become the current development of spirulina. Hotspots in the research field. At present, hot water method, cold alkali method, etc. are mostly used for the extraction of Spirulina polysaccharides. These methods all have disadvantages such as time-consuming, energy-consuming, and low yield. [0003] Microwave extra...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 杨劲峰吴文惠傅诗情
Owner RONGCHENG GUANGRUN SEAFOOD CO LTD
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