Method for producing trichoderma chlamydosporium
A technique for chlamydospore and production method, which is applied in the directions of botanical equipment and methods, fungi, biocides, etc., can solve the problems of short commodity price period, short spore survival period, unstable control effect, etc., and achieve the survival time. Long-term, simple and easy to implement, competitive in the soil and the effect of high reproductive speed
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Embodiment 1
[0021] 1. Preparation of slant mother strains
[0022] Take 5 grams of crop root surface soil, add 50 ml of sterile water, dilute 1000 times after shaking to form a dilution, take 0.1 ml of the dilution and 10 ml of the mother strain culture solution and pour it into a petri dish to make a culture medium plate. Among them, the culture solution of the mother strain is taken: 150 grams of potatoes, 20 grams of glucose, 0.05 grams of Bengal red, 0.03 grams of streptomycin sulfate, 17 grams of agar, and 1000 ml of water. The production method is: peel the potatoes and cut them into 1 cm squares Add 1000 ml of water and boil for 20 minutes, filter the potato pieces with gauze, add agar, add water to make up 1000 ml, then add glucose and Bengal red and make it completely melted, then put it into a triangular flask for sterilization, at 121 °C Sterilize for 30 minutes and add streptomycin sulfate before inverting the plate. Cultivate at 25°C for 48 hours, obtain the Trichoderma stra...
Embodiment 2
[0029] 1. Preparation of slant mother strains
[0030] Take 10 grams of crop root surface soil, add 100 ml of sterile water, dilute 10,000 times after shaking to form a dilution, take 1 ml of the dilution and 15 ml of the mother strain culture solution and pour it into a petri dish to make a culture medium plate. Among them, the culture solution of the mother strain is taken: 200 grams of potatoes, 20 grams of glucose, 0.06 grams of Bengal red, 0.04 grams of streptomycin sulfate, 20 grams of agar, and 1000 milliliters of water. The production method is: peel the potatoes and cut them into 1 cm squares Add 1000 ml of water and boil for 30 minutes, filter the potato pieces with gauze, add agar, add water to make up 1000 ml, then add glucose and Bengal red and make it completely melted, then put it into a triangular flask for sterilization, at 121 °C Sterilize for 30 minutes and add streptomycin sulfate before pouring the plate. Cultivate at 28°C for 48 hours, obtain the Trichod...
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