Efficient goat breeding method based on meat properties

Abstract

The invention relates to the technical field of breeding of livestock and poultry, and especially relates to an efficient goat breeding method based on meat properties. The method comprises the following steps: determination on body weight, DNA sample acquisition, primer synthesis, template PCR amplification of a primer, genotype determination and genotyping, sequencing verification on individual PCR products with different genotypes, individual MBLUP heredity assessment, matching and establishing of a family, formation of a core colony through colony continuous-progeny selection, and manual dispersion of excellent genes. By determining body weight and detecting molecular marker genotype, MBLUP technology is employed for evaluating breeding value of breeding goats so as to perform selection, body-weight determination and genotype detection are performed on selected progeny according to a designed selection target, and the MBLUP method is used to perform colony continuous-progeny selection, so that finally a new variety of north Guizhou Ma goat for meat is formed. By taking selection of north Guizhou Ma goat as an example, the body weight of rams and ewes in a selected north Guizhou Ma goat core colony for meat is improved by 14.12 Kg and 9.61 Kg compared with a basic colony.

Description

technical field [0001] The invention relates to the technical field of animal breeding, in particular to a rapid and efficient breeding method for goat meat traits. Background technique [0002] The best linear unbiased prediction BLUP is a breeding value estimation method developed in the middle and late 1980s, and has become an advanced genetic evaluation method generally recognized by scholars at home and abroad; in the existing technology, the best The method of linear unbiased prediction and estimation of breeding value is applied to the breeding of aquatic species of livestock and poultry. The reports on the application of this method to aquatic species include journal papers and 5 patent documents. The best linear unbiased prediction breeding value estimation method has obvious advantages over other breeding value estimation methods: its breeding is aimed at breeding value rather than phenotype, stripping off environmental effects, so the selection efficiency is highe...

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Problems solved by technology

[0003] Marker-assisted BLUP (MBLUP) breeding technology is mainly used in aquatic species at home and abroad, but has not been applied to mammals; goats, as a kind of mammals, are also an important part of my country's livestock and poultry genetic resources. It has the advantages of strong adaptability, resistance to rough feeding, high reproduction rate, good meat production performance, light smell of mutton, and delicious meat; but goat breeds still have technical problems such as slow growth and low economic benefits. At the same time, a goat breed Germplasm protection is a long-term work. The lack of economy and scattered resources make goat breeds unable to escape the fate of continuous degradation of genetic quality

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