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Method for building pedigree of marsupenaeus japonicus and breeding

A technology of pedigree and prawns, applied in fish farming, application, climate change adaptation, etc., can solve problems affecting credibility, lack of half-sib pedigree information, etc., to avoid inbreeding, high success rate, and reduce manual operations Effect

Inactive Publication Date: 2012-04-11
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the lack of half-sib pedigree information affects credibility at the level of individual selection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: the construction of basic group

[0024] Collect 50,000 wild shrimp seedlings from the vicinity of Zhao'an Bay in Fujian Province, and preliminarily select 10,000 shrimp seedlings with basically the same specifications, and put them in a 20m 2 Temporary cultivation in cement ponds, during the temporary cultivation process, the carrier status of WSSV and other viruses was detected. When it is determined that these batches of shrimp are free of virus, and after observing in the pond for one week, move the shrimp to a shrimp pond of about 1 mu for artificial breeding. The 300 prawns continue to be cultivated as mature broodstock for natural mating.

Embodiment 2

[0025] Example 2: F 1 Generation family construction and selection

[0026] (1)F 1 Generation family construction: select fertilized female shrimp with sperm pods and well-developed gonads from the broodstock pond 150 prawns, eye stalks were cut for gonadal maturation and artificial induction, and each shrimp was laid eggs separately, and the number of eggs laid and hatching rate were calculated, and the families with less eggs laid and hatching rate were eliminated, and the remaining seed families were charged at a rate of 0.5 m 3 3,000 nauplii were raised in the same bucket, with sufficient bait and well-controlled water quality, and 60 families of all compatriots were cultivated. When the shrimp seedlings are bred to P 10 After period, each 5m 2 Quantitative stocking of 600 shrimp seedlings in the ponds of the same species was carried out in standardized sub-pond cultivation.

[0027] (2) Cultivation and trait testing in the same pond: After the shrimp seedlings are ...

Embodiment 3

[0028] Embodiment 3: the construction of cousin family lineage

[0029] (1) Construction of the family line of cousins: from 1 F 1 30 remaining male shrimps were selected from the generation family, and 30 female shrimps were selected from the other 10 families, that is, 3 female shrimps with well-developed gonads were selected from each family for fluorescent labeling, and then the ratio of male to female was 1:1. These 60 shrimps were put together for breeding in the same pond and mated naturally. The method of embodiment 2 selects and stays the F that laying eggs amount is big, hatching rate is high 2 generation family. F 1 All F 2 The offspring form a full-sibling family with a common F 1 surrogate parents; different F 1 F 2 The offspring have a cousin relationship, that is, their F 1 The male parents are from the same family with a common F 0 Grandparents; have the same F 0 all the F's for grandparents 2 The grandchildren constitute a family line of cousins. T...

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Abstract

A method for building pedigree of marsupenaeus japonicus and breeding relates to the breeding of the penacus orientalis. The method comprises the following steps: building the base population; building the F1 generation pedigree; cultivating in a same pond; building the F2 generation close cousins pedigree; after growing to 3 cm, cultivating in the same pond, and selecting the breeding parents while reaching the specification of goods to generally build 8-16 close cousins pedigrees, wherein each close cousins pedigree retains 5-8 fullsibs family, the total number of the breeding pedigrees is 60-80, and the breeding parents in each pedigree are continuously bred to mature parents; and adopting a continuous multi-generation hybrid system to continuously build the close cousins pedigree of the next generation until to reach the breeding target and form the new fast growing variety. The invention is a method for building close cousins pedigree of marsupenaeus japonicus and breeding, especially can be applied to the inheritance breeding of the marsupenaeus japonicus, and has important significance for the breeding design of the marine animals which are not suitable for the fine breeding mode.

Description

technical field [0001] The invention relates to the breeding of prawns, in particular to a family construction and breeding method of Penaeus japonicus. Background technique [0002] Improved species are an important basis for the sustainable development of aquaculture. Litopenaeus vannamei, an excellent species, has created an output value of more than 100 billion yuan, contributing about 40% of the output to the world's prawn farming. In 2004, my country bred the first new species of maricultured animals - Chinese prawn "Huanghai No. 1", which wrote a new chapter for my country's research on prawn germplasm innovation. [0003] As an important means of improving animal species, family selection has played an important role in the research of prawn breeding. It has bred Chinese shrimp "Huanghai No. 2", Litopenaeus vannamei "Kehai No. 1", "Zhongke No. 1" and "Zhongxing No. 1" and many other new varieties. Breeding value estimation and genetic evaluation are important cont...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/027A01K61/00
CPCY02A40/81
Inventor 毛勇王军苏永全
Owner XIAMEN UNIV
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