The invention discloses a transgenic vector for target
mutation of primordial germ cells, a method for preparing the transgenic vector and application thereof. The transgenic vector, the method and the application have the advantages that the vector pTol2 (UAS: Cas9) is introduced into
zebrafish embryos at unicellular stages to obtain strains Tg (UAS: Cas9) and strains Tg (kop: KalTA4), and Cas9 genes can be efficiently, specifically and continuously expressed in the primordial germ cells of zebrafishes by
hybrid embryos obtained after fishes of the strains Tg (UAS: Cas9) and female fishes of the strains Tg (kop: KalTA4) are hybridized; the
hybrid embryos of the male fishes of the strains Tg (UAS: Cas9) and the female fishes of the strains (kop: KalTA4) are used as
receptor embryos when
mutation strains are about to be prepared, somatic
mutation can be effectively reduced after gRNA [
guide RNA (ribonucleic acid)] of target genes is directly injected, accordingly, the
survival rate of the injected P0-generation embryos can be increased, P0-generation adult fishes can effectively pass on mutation, and accordingly the transgenic vector and the method have broad application prospects.