34 results about "Variant virus" patented technology

The invention relates to the technical field of porcine pseudorabies viruses (PRVs) and in particular relates to a porcine PRV variant PRV-ZJ01 with collection number of CGMCCNo.8170 and an application of the porcine PRV variant PRV-ZJ01 in preparation of vaccines. The porcine PRV variant PRV-ZJ01 has the beneficial effects that a water-soluble inactivated vaccine is prepared by adopting a PRV-ZJ01 variant virus solution and is subjected to a swine immune protection test with live vaccines of Bartha-K61, Bucharest and HB-98 strains and the results show that the inactivated vaccine of the ZJ01 strain has relatively high safety and has the immune protection efficiency obviously higher than that of immunity groups of the live vaccines of the Bartha-K61, Bucharest and HB-98 strains, and the live vaccines of the Bartha-K61, Bucharest and HB-98 strains can not provide full protection for the ZJ01 very virulent strain; the inactivated vaccine of ZJ01 has relatively good immune protection effects on the PRV variant and the traditional strains; infected with 10<6.0>TCID50 (Tissue culture infectious dose 50) / ml nasal drops of the PRV-ZJ01 variant, all the 85-day-old non-immune swine can become ill and die; results prove that the virulence of the virus strain is obviously enhanced, the antigenicity is varied and the virus strain has relatively good immunogenicity after being inactivated and can be used for research and development of the vaccine of the virus strain and the diagnostic methods.

The invention relates to the technical field of porcine pseudorabies viruses and especially relates to a gE- and gI-deleted porcine pseudorabies virus variant strain PRV-ZJ011G and a use thereof. The gE- and gI-deleted porcine pseudorabies virus variant strain PRV-ZJ011G has the accession number of CGMCC No.7957. The invention discloses the use of the gE- and gI-deleted porcine pseudorabies virus variant strain PRV-ZJ011G in vaccine preparation. After the New Zealand big white rabbit is inoculated with the 106.0TCID50 recombinant viruses, clinical symptoms such as pruritus are not caused. An oil-in-water inactivated vaccine prepared from the gE- and gI-deleted porcine pseudorabies virus variant strain PRV-ZJ011G is injected into a piglet and after four weeks, the BELISA antibody is produced but the gE antibody does not exist, and the immunization protection efficiency is 100%. After immunization on sows, the piglets produced by the sows get immunization protection and the efficiency of PRV variant virus and traditional virus immunization protection is 100%. It is proved that the ZJ011G recombinant virus has good immunogenicity and can be used for vaccine preparation.

A process of preparing single-colon antibody resisting surface antigen of hepatitis B virus mutant from CGMCCHBSP2. The single-colon antibody can detect 14 types hepatitis B virus containing mutant and wild strain and replace HBsAb in fluorogence diagnostic kit. It can avoid mistake induced by normal agent and be used widely.

The invention relates to a nucleoside compound and application thereof, in particular to a compound shown in a formula I, a prodrug thereof and / or pharmaceutically acceptable salt thereof, and a preparation method, a composition and application thereof. The compound and the composition have the application of preventing, relieving and / or treating coronavirus infection, or replication or reproduction of homologous variant viruses of the coronavirus infection, and cytopathic effects generated by the coronavirus infection or the replication or reproduction of the homologous variant viruses of the coronavirus infection.

The invention discloses a matrix protein mutated recombinant vesicular stomatitis virus serving as a porcine vaccine vector. The vaccine vector is a recombinant virus VSV (vesicular stomatitis virus) delta M51. The invention also provides a kit for detecting the specific M antibody in porcine serum. The using method of the kit comprises the following steps: establishing an ELISA method by utilizing high-purity M protein prepared by recombinant expression to detect the level of the specific M antibody in porcine serum which is inoculated with VSV delta M51 virus. The invention firstly provides the variant virus strain (VSV delta M51) of which the 51st arginine of the matrix protein of an VSV critical virulence factor serving as a candidate VSV virus strain with application values, provides a systematic evaluation to the pathogenicity of VSV delta M51 on pigs for the first time at home and abroad, and provides basis to VSV delta M51 serving as the vaccine vector.

The present invention provides a mNGS-based method for detecting COVID‑19 novel coronavirus and its application. In the method, multiple genome-specific reverse transcription primer sets for COVID‑19 novel coronavirus are designed and prepared to improve the detection of novel coronavirus RNA. Reverse transcription efficiency reduces the problem of aerosol pollution, and also improves the ability to enrich nucleic acid sequences of different mutant viruses.

The invention belongs to the technical field of RNA vaccines, and particularly relates to an RNA vaccine for porcine epidemic diarrhea and a construction method thereof. According to construction of the RNA vaccine, porcine 5' UTR and 3' UTR are selected to be connected with a S protein, termination codon and polyA nucleotide sequence of a strain in series to obtain the RNA vaccine; by adopting a lipid nanoparticle as a delivery carrier of the RNA vaccine, a novel porcine epidemic diarrhea (PEDV) strain of a current epidemic wild strain can be rapidly coped, and the novel PEDV strain can be efficiently and rapidly targeted. The RNA vaccine for the porcine epidemic diarrhea, prepared by the invention, is low in cost and high in production efficiency, and can induce cellular immunity and humoral immunity at the same time; the prepared lipid nanoparticle-encapsulated RNA vaccine can rapidly cope the new PEDV strain of the current epidemic wild strain, especially a variant virulent strain, so that the research, development and production cycle of new vaccine development is shortened, and an effective technical means is provided for prevention and control of a new variant virulent strain.

The invention belongs to the technical field of biology, and particularly relates to a qRT-PCR (quantitative reverse transcription-polymerase chain reaction) method for identifying a novel coronavirus Omicro variant BA-2 branch. According to the method, in order to improve the detection sensitivity and specificity, a TaqMan probe is introduced; in order to reduce the cost, two pairs of primers are changed into one pair of half primers, that is, two upstream primers respectively target a mutation site and an original non-mutated site, and one downstream primer is shared by the two upstream primers. By reducing the matching degree between the variation primer and the non-variation virus nucleic acid and the matching degree between the non-variation primer and the variation virus nucleic acid in the reaction system, the amplification curve of the variation primer for amplifying the variation virus nucleic acid in the reaction system is earlier than the amplification curve of the non-variation nucleic acid; the amplification curve of the non-variant nucleic acid amplified by the non-variant primer is earlier than the amplification curve of the variant nucleic acid amplified by the non-variant primer.

The invention relates to a Japanese encephalitis virus non-structural protein NS1 truncated mutant, its coding gene and application, and belongs to the technical field of viruses. The non-structural protein NS1 truncation mutant of Japanese encephalitis virus deletes two strong hydrophobic regions at the C-terminus on the basis of the original NS1, and obtains a truncation mutant NS1 with 63 amino acids at the C-terminus deleted △63 Gene, cloned into the prokaryotic expression vector pET‑28a(+), and obtained the recombinant expression vector pET28a‑NS1 △63 , transformed into E. coli, induced by IPTG, NS1 △63 It can be expressed efficiently and stably in Escherichia coli. NS1 expressed in E. coli △63 After protein purification and immunization of mice, the titer of NS1 antiserum of immunized mice reached 1:12150 by ELISA. Protection tests in immunized mice showed that NS1 △63 Has protective activity. The NS1 △63 The protein can lay the foundation for the development of later virus vaccines and diagnostic kits.

A monoclonal antibody against mutant hepatitis B virus surface antigen secreted by hybridoma cell line CGMCC HBSP2. The anti-mutated hepatitis B surface antigen monoclonal antibody prepared according to the present invention can simultaneously detect hepatitis B viruses including 14 kinds of mutant strains and wild strains, and can replace the HBsAb in the conventional hepatitis B surface antigen detection kit. Into the hepatitis B surface antigen diagnostic kit. The use of the antibody can avoid the missed diagnosis caused by conventional reagents, so that the carriers of the mutated and non-mutated hepatitis B virus or hepatitis B patients can be diagnosed and treated in time. Especially when screening blood donors, it can detect mutant virus carriers that cannot be detected by conventional kits, avoid transfusing the blood of hepatitis B patients as healthy blood to recipients, and ensure the quality of blood sources.

The invention discloses a pure traditional Chinese medicine prescription composition used for purifying breeding sow disease-free pig farms, and avoiding death of sucking pigs with dysentery caused byvirus mutants and viruses carried by breeding sows. The pure traditional Chinese medicine prescription composition is composed of following raw materials via processing: radix bupleuri, dried FructusForsythiae, mulberry leaf, great burdock achene, reed rhizome, gentian, Flos Eriocauli, lophatherum gracile, cordate houttuynia, patrinia herb, pale butterfly bush flower, cassia seed, coptis chinensis, oriental waterplantain rhizome, Herba Artemisiae scopariae, rheum officinale, Ligusticum wallichii, motherwort, rhizoma corydalis, herba epimedii, sophora bud, Herba Agrimoniae, sanguisorba officinalis, artificially cultured Radix Scutellariae, artificially cultured Radix Astragali, bighead atractylodes rhizome, magnolia cortex, and licorice. According to a preparation method, the above 28 traditional Chinese medicinal materials are prepared into powder via different processing methods (stir-baking with vinegar, wine, or ginger juice), the pure traditional Chinese medicine prescription composition is capable of solving a problem that pure traditional Chinese medicines are not accepted by breeding sows because of the obvious bitter taste, and avoiding death of sucking pigs with dysentery caused by virus mutants and viruses carried by breeding sows, and is safe and reliable; no toxic or side effect is caused; and no drug residue is left.