RNA vaccine for porcine epidemic diarrhea and construction method thereof

Abstract

The invention belongs to the technical field of RNA vaccines, and particularly relates to an RNA vaccine for porcine epidemic diarrhea and a construction method thereof. According to construction of the RNA vaccine, porcine 5' UTR and 3' UTR are selected to be connected with a S protein, termination codon and polyA nucleotide sequence of a strain in series to obtain the RNA vaccine; by adopting a lipid nanoparticle as a delivery carrier of the RNA vaccine, a novel porcine epidemic diarrhea (PEDV) strain of a current epidemic wild strain can be rapidly coped, and the novel PEDV strain can be efficiently and rapidly targeted. The RNA vaccine for the porcine epidemic diarrhea, prepared by the invention, is low in cost and high in production efficiency, and can induce cellular immunity and humoral immunity at the same time; the prepared lipid nanoparticle-encapsulated RNA vaccine can rapidly cope the new PEDV strain of the current epidemic wild strain, especially a variant virulent strain, so that the research, development and production cycle of new vaccine development is shortened, and an effective technical means is provided for prevention and control of a new variant virulent strain.

Description

technical field [0001] The invention belongs to the technical field of RNA vaccines, and in particular relates to an RNA vaccine for porcine epidemic diarrhea and a construction method thereof. Background technique [0002] Porcine epidemic diarrhea (PED) is a common, acute and highly prevalent porcine enterovirus infectious disease, the pathogen is porcine epidemic diarrhea virus (PEDV). The main clinical symptoms of the disease are diarrhea, including watery diarrhea, and other symptoms including vomiting, anorexia, dehydration, and weight loss. The clinical changes and symptoms are similar to those of porcine transmissible gastroenteritis (TGE). One of the major diseases causing death. [0003] The disease was first reported in the United Kingdom in 1971, and then European countries began to spread one after another. my country first reported the disease in 1976. Since the late 1980s, PED has spread in various regions of China, and began in 1995. Combined prevention of p...

AI Technical Summary

Problems solved by technology

[0006] The early prevention and control of PED mainly used tissue inactivated vaccines, but the virus content of tissue inactivated vaccines is low, the immunity persistence is not strong, and often requires multiple immunizations, so it is not conducive to large-scale immunization of large-scale farms; there are many kinds of vaccines Including cell-inactivated vaccines, attenuated vaccines and subunit vaccines, all have good immune effects on the virus strains, but the reason why PED can be popular in the world and cause huge economic losses to farmers is precisely because of the genotype types of PEDV strains. Numerous and prone to genetic mutations, making the strains more virulent, so it is difficult for existing vaccines to exert protective effects

Domestic porcine epidemic diarrhea virus strains have not only mutated to form new strains, but also there are genetic differences among the strains, forming a diversity of gene sources. These factors can cause vaccine failure and epidemics

The development of traditional inactivated vaccines and attenuated vaccines requires a certain period of time, and the speed of vaccine development often cannot keep up with the mutation speed of viruses

Images