Matrix protein mutated recombinant vesicular stomatitis virus serving as porcine vaccine vector

Abstract

The invention discloses a matrix protein mutated recombinant vesicular stomatitis virus serving as a porcine vaccine vector. The vaccine vector is a recombinant virus VSV (vesicular stomatitis virus) delta M51. The invention also provides a kit for detecting the specific M antibody in porcine serum. The using method of the kit comprises the following steps: establishing an ELISA method by utilizing high-purity M protein prepared by recombinant expression to detect the level of the specific M antibody in porcine serum which is inoculated with VSV delta M51 virus. The invention firstly provides the variant virus strain (VSV delta M51) of which the 51st arginine of the matrix protein of an VSV critical virulence factor serving as a candidate VSV virus strain with application values, provides a systematic evaluation to the pathogenicity of VSV delta M51 on pigs for the first time at home and abroad, and provides basis to VSV delta M51 serving as the vaccine vector.

Description

technical field [0001] The invention relates to a recombinant vesicular stomatitis virus with matrix protein mutation as a porcine vaccine carrier. Background technique [0002] Vesicular stomatitis (VS) is an acute highly contagious disease caused by vesicular stomatitis virus (VSV). Horses, cattle, pigs and other animals are more susceptible, and sheep and goats are also susceptible. Can be infected. Clinically, blisters occur on the epithelium of the tongue, lips, oral mucosa, nipples, and hooves as the main feature. Vesicular stomatitis is clinically difficult to distinguish from infectious diseases such as foot-and-mouth disease (FMD), vesicular disease of swine (SV), and vesicular eruption of swine (VES). In my country, vesicular stomatitis is an exotic animal disease, and no large-scale outbreak has been reported. It is listed as a second-class disease in the country's entry and exit animal quarantine objects. However, with my country's entry into the WTO, the inter...

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Problems solved by technology

First of all, mice are not the natural host of VSV, and usually do not show significant pathogenicity, but under experimental conditions, artificial inoculation, especially high-dose nasal drip, can cause them to become diseased; VSV is an arbovirus, In bovine animals such as pigs and cattle, VSV can be transmitted through contact routes and exhibit high pathogenicity and even lethality; secondly, VSV shows neurotropism in mice, which can cause hindlimb paralysis of animals, while in pigs and other natural On the host, it mainly exhibits epitheliophilia, which can cause severe vesicular lesions in the mouth and other places

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