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Stationary liquid for microscopic observation of hypsizigus marmoreus basidiospores, preparation method, fixing method and application

A technology of microscopic observation and fixation method, applied in the field of biological histology, can solve the problems affecting the research work of basidiospores of shiji mushroom

Active Publication Date: 2021-01-29
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, the Brownian motion of the basidiospores of jijiji mushroom is the most serious, because compared with other edible fungi, the basidiospores are smaller and have a long diameter of about 5 μm. In microscopic observation, especially under high-power lenses, the basidiospores move irregularly. Especially obvious, it has seriously affected the basidiospore research work of Shiji mushroom

Method used

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  • Stationary liquid for microscopic observation of hypsizigus marmoreus basidiospores, preparation method, fixing method and application
  • Stationary liquid for microscopic observation of hypsizigus marmoreus basidiospores, preparation method, fixing method and application
  • Stationary liquid for microscopic observation of hypsizigus marmoreus basidiospores, preparation method, fixing method and application

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specific Embodiment approach 1

[0048] This particular embodiment provides a fixative solution for microscopic observation of fungal basidiospores. The fixative solution includes gelatin and glycerin hydration agent, wherein the mass volume concentration of gelatin is 20% to 40%, and glycerin in glycerin hydration agent The volume ratio with water is 1:1.

specific Embodiment approach 2

[0050] This specific embodiment provides a method for preparing the fixative described in Embodiment 1. The preparation method includes adding gelatin to glycerin hydration agent and heating to melt the gelatin, and mixing to obtain the fixative. The heating temperature is 50-50°C. 65°C.

specific Embodiment approach 3

[0052] This specific embodiment provides a method for fixing the fungal basidiospores by using the fixative prepared in the second specific embodiment, comprising the following steps:

[0053] (1) Preparation of fungal basidiospore suspension

[0054] Use a pipette to draw 200 μl of sterile water on the basidiospore print of the fungus for pipetting and washing, and put the sterile water mixed with the basidiospores of the fungus into a 1.5ml centrifuge tube to make a suspension of the basidiospores of the fungus.

[0055] (2) Staining of fungal basidiospores

[0056] Centrifuge the fungal basidiospore suspension obtained in step (1) at 3000rpm, remove the supernatant, add 100μl of calcofluor white (1g / l) staining solution, store in the dark for 10min, add excess PBS solution, and shake and wash Afterwards, remove the supernatant.

[0057] (3) Fixation and observation of samples

[0058] Add the fixative prepared in Embodiment 2 to the fixed sample after staining in step (2...

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Abstract

The invention discloses a stationary liquid for microscopically observing hypsizigus marmoreus basidiospores, a preparation method, a fixing method and application. The stationary liquid provided by the invention is prepared by mixing molten gelatin and a glycerol hydration agent. The stationary liquid obtained by the method is good in light transmission and safe in phase change temperature, and the method is simple, convenient and rapid, can be used for batch production, and can also be used for temporary preparation in case of urgent need. The fixing liquid is used for fixing the hypsizigusmarmoreus basidiospores, other treatment effects such as dyeing of the hypsizigus marmoreus basidiospores are not affected, the exposure time can be prolonged, and the quality of obtained hypsizigus marmoreus basidiospore image data is improved.

Description

technical field [0001] The invention relates to the technical field of biological histology, in particular to a fixative, a preparation method, a fixation method and an application for the microscopic observation of the basidiospores of P. Background technique [0002] Hypsizygus marmoreus (Peck) H.E.Bigelow, also known as jade mushroom and spot jade mushroom, belongs to Basidiomycotina, layer fungi, agaricaceae white mushroom family, jade mushroom genus, beautiful appearance, crisp and tender texture, delicious taste, is Important industrial cultivation of edible fungi. At present, there are two strains of Shiji mushroom that can be artificially cultivated: brown and pure white. The brown is usually called "crab-flavored mushroom"; the pure white is called "seafood mushroom" or "white jade mushroom". Basidiospores are the initial reproductive units of edible fungi, and the life history of most edible fungi consists of the process of germination, fusion, and fruiting of bas...

Claims

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Application Information

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IPC IPC(8): G01N1/30G01N21/64
CPCG01N1/30G01N21/6458G01N2001/305
Inventor 万佳宁周陈力鲍大鹏杨瑞恒李燕龚明王莹陈洪雨候娣
Owner SHANGHAI ACAD OF AGRI SCI
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