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Application of farnesol in preventing and treating smut disease

A technology for farnesol and smut, which is applied to the application field of farnesol in the prevention and control of smut, can solve problems such as the inability to effectively prevent and control diseases, and achieve the effect of realizing green and sustainable development.

Active Publication Date: 2018-09-21
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the measures adopted to control sugarcane smut and corn smut include breeding and popularizing disease-resistant varieties and chemical control, but the current control measures cannot effectively control the disease

Method used

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  • Application of farnesol in preventing and treating smut disease
  • Application of farnesol in preventing and treating smut disease
  • Application of farnesol in preventing and treating smut disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] On the basis of the preliminary experiment, YEPS liquid medium was used to configure farnesoid into a mother solution with a certain concentration gradient (0 μM, 2.5 μM, 5 μM, 10 μM) for later use. Scrape teliospores from collecting fresh sugarcane smut standard sample again, make teliospore suspension (5×10) with YEPS liquid medium 4 pieces / ml). Pipette 200 μl of different concentrations of mother liquor into 2ml sterilized centrifuge tubes, add an equal volume of teliospore suspension and mix well, and use YEPS without drug as a control. Three repetitions were set for each drug concentration, and each treatment was placed in a shaker at 28°C and 200 rpm / min for 6 hours. After the germination of each teliospore was examined under a microscope, the number of teliospores germinated in 100 teliospores was recorded, and the inhibition rate was calculated.

[0034] Experimental results (see figure 1 ), after culturing in a shaker for 6 hours, the germination rate of the ...

Embodiment 2

[0036]On the basis of the preliminary experiment, the YEPS liquid medium for farnesol was prepared into a mother solution with a certain concentration gradient (0 μM, 0.5 μM, 0.8 μM, 1 μM, 2 μM, 4 μM, 6 μM, 8 μM, 9 μM, 10 μM, 20 μM) for later use. . Pick a little sugarcane smut basidiospores from the flat plate and cultivate overnight in 6mlYEPS nutrient solution as seed liquid, centrifuge the next day to collect the thalline, and resuspend the thalline with YEPS liquid medium (OD 600 0.2) for use. Pipette 200 μl of different concentrations of mother liquor into 2ml sterilized centrifuge tubes, add the same amount of basidiospore suspension and mix well, and use the YEPS liquid medium without adding drugs as the control, and set three replicates for each concentration treatment. Then measure the initial OD of each treatment with a spectrophotometer 600 Afterwards, cultured in a shaker at 28°C and 200rpm / min for 20h, and measured the final OD of each treatment 600 , by calcu...

Embodiment 3

[0041] On the basis of the preliminary experiment, YEPS liquid medium was used to configure farnesoid into a mother solution with a certain concentration gradient (0 μM, 8 μM, 12 μM, 16 μM, 20 μM) for later use. Pick a little sugarcane smut and maize smut basidiospores from the plate respectively and culture them overnight in 6ml YEPS culture solution as the seed solution, and transfer 500 μl to 50ml YEPS culture solution to expand the culture the next day (to OD 600 0.2), pipette 500 μl of different concentrations of mother solution into 5ml sterilized centrifuge tubes, add an equal volume of bacterial solution and mix well, place in 28°C, 200rpm / min shaker for 12h. Then use fresh YEPS liquid medium to dilute the bacterium solution of each concentration treatment by 10 4 times, draw 200 μl and spread it on the YEPSA solid medium, and place it in a 28°C incubator for static culture for 48h. Finally, observe the antibacterial effect and count the number of colonies.

[0042] ...

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Abstract

The invention discloses an application of farnesol in preventing and treating the smut disease of sugarcane and corn. According to the application of farnesol in preventing and treating smut disease,the life history of sugarcane smut can be affected. Farnesol has better inhibiting effect on the germination of teliospore, the proliferation and growth of basidiospore and the sexual cooperation. Theformation of the binuclear mycelium can be effectively blocked, the sugarcane can not be normally infected with sugarcane smut, and the occurrence of the smut disease of the sugarcane can be effectively inhibited; the proliferation and growth of corn smut can be affected and the occurrence of corn smut can be inhibited.

Description

technical field [0001] The invention relates to the technical field of biological control, in particular to the application of farnesol in the prevention and treatment of smut. Background technique [0002] Smut is mainly a plant disease caused by smut. According to incomplete statistics, there are about 1,200 species of smut, which can infect more than 4,000 kinds of plants. Their hosts include grass plants and important crops in the world such as: Corn, sugarcane, barley, wheat, oats, sorghum, etc. Smut is characterized by the formation of piles of black powdery teliospores on the tissue of the infested host. [0003] Corn and sugarcane are the main food crops in the world, and they are also important biomass energy materials and feed crops. Maize smut caused by Ustilago maydis and sugarcane smut caused by Sporisorium scitamineum are the main diseases affecting the yield and quality of the two crops respectively. At present, the measures adopted to control sugarcane smu...

Claims

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Application Information

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IPC IPC(8): A01N49/00A01P3/00
CPCA01N49/00
Inventor 常长青仇善旭姜子德张炼辉蔡恩平吴熔熔王雨婷
Owner SOUTH CHINA AGRI UNIV
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