The invention relates to the technical field of biomedical research, and in particular relates to a construction method of a bone defect model manufactured by adopting osteotomy on bilateral fibula ofmacaca fascicularis. The technical scheme is as follows: the method comprises the following steps: under the aseptic condition, carrying out anesthetization on an animal, on the limb under the surgery, processing a small stabbing incision at the tail end of the fibula by adopting a scalpel, inserting a kirschner wire from the tail end of the fibula, and after fixing, inserting the kirschner wireinto a marrow cavity of the fibula for 2cm; and later, processing an incision on the fibula, carrying out blunt dissection on the muscle on the fibula, so that the fibula is exposed, pulling open thesoft tissue surrounding the fibula, marking the part needing osteotomy, cutting off the part with the length being 1.5cm on the fibula, after the osteotomy is completed, checking the bone breakage end, further inserting the kirschner wire placed at the tail end of the fibula so that the kirschner wire stretches over the cut-off part, cutting off the part, exposed out of the far-end fibula, of thekirschner wire, and pegging the broken end after cutting into the fibula by using a punch and a hammer for the orthopedics department. For the method provided by the invention, experimental data morerelevant to human beings is provided for exploring the disease cause of bone defect and providing an effective clinical treatment scheme.