Construction method of bone defect model manufactured by adopting osteotomy on bilateral fibula of macaca fascicularis

A construction method and osteotomy technology, applied in the field of bone defect model construction, can solve problems such as limiting the progress of medical technology

Inactive Publication Date: 2019-01-22
澎立生物医药技术(上海)股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The drug effects observed in rodents and primates are often very different from those in humans, which limits the progress of medical technology to a certain extent

Method used

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  • Construction method of bone defect model manufactured by adopting osteotomy on bilateral fibula of macaca fascicularis
  • Construction method of bone defect model manufactured by adopting osteotomy on bilateral fibula of macaca fascicularis
  • Construction method of bone defect model manufactured by adopting osteotomy on bilateral fibula of macaca fascicularis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1. Experimental Animals

[0021]

[0022] Raise the experimental animals in a normal-grade animal room and raise them in single cages. Keep the indoor temperature at 20-29°C, relative humidity at 40-70%, fluorescent lamps for 12-hour cycle lighting, and the lighting time is 08:00-20:00. Animals are free Feed and drink freely, and the drinking water is tap water purified by a water purifier, which complies with WHO drinking water regulations.

Embodiment 2

[0023] Example 2. Anesthesia

[0024] First intramuscular injection of 0.05mg / kg atropine. After 5-10 minutes, intramuscular injection of 4 mg / kg ketamine was performed for anesthesia. Thereafter, anesthesia was maintained with 0.8-1.5 L / min oxygen containing 1.5-5% isoflurane.

Embodiment 3

[0025] Example 3. Surgical procedure

[0026] On the day when the animals underwent surgery, the hair on the surgical extremities was shaved, and the perioperative area was cleaned and disinfected with povidone iodine and 70% ethanol. Then the experimental monkey was brought into the operating room and placed in the supine position. Suspend and fix the surgical limb around, and use povidone iodine to disinfect and prepare the skin at 360 degrees. Sterile surgical drapes were placed on the proximal end of the surgical extremity and the trunk. Surgical limbs and feet are wrapped with sterile elastic bandages to ensure sterility throughout the operation. The operated limb was then immobilized. Then perform the same procedure on the contralateral limb. After both feet of the experimental monkey were wrapped, the body was covered with a surgical drape that had been opened in advance for the feet to protrude.

[0027] On the operated limb, a small stab incision is made with a s...

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Abstract

The invention relates to the technical field of biomedical research, and in particular relates to a construction method of a bone defect model manufactured by adopting osteotomy on bilateral fibula ofmacaca fascicularis. The technical scheme is as follows: the method comprises the following steps: under the aseptic condition, carrying out anesthetization on an animal, on the limb under the surgery, processing a small stabbing incision at the tail end of the fibula by adopting a scalpel, inserting a kirschner wire from the tail end of the fibula, and after fixing, inserting the kirschner wireinto a marrow cavity of the fibula for 2cm; and later, processing an incision on the fibula, carrying out blunt dissection on the muscle on the fibula, so that the fibula is exposed, pulling open thesoft tissue surrounding the fibula, marking the part needing osteotomy, cutting off the part with the length being 1.5cm on the fibula, after the osteotomy is completed, checking the bone breakage end, further inserting the kirschner wire placed at the tail end of the fibula so that the kirschner wire stretches over the cut-off part, cutting off the part, exposed out of the far-end fibula, of thekirschner wire, and pegging the broken end after cutting into the fibula by using a punch and a hammer for the orthopedics department. For the method provided by the invention, experimental data morerelevant to human beings is provided for exploring the disease cause of bone defect and providing an effective clinical treatment scheme.

Description

technical field [0001] The invention relates to the technical field of biomedical research, in particular to a method for constructing a bone defect model produced by bilateral fibula osteotomy in cynomolgus monkeys. Background technique [0002] Bone defect refers to the destruction of the structural integrity of the bone, which is a common clinical disease and one of the difficult problems in orthopedic treatment. At present, the methods for treating bone defects include autologous bone and allogeneic bone transplantation, tissue engineering technology, gene therapy, growth factor, and adjuvant therapy of physical therapy. Trauma, infection, tumor, surgical debridement of osteomyelitis, and various congenital diseases are the main causes of bone defects. The drug effects observed in rodents and primates are often very different from those in humans, which limits the progress of medical technology to a certain extent. [0003] Therefore, it is urgent to establish a bone d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D1/00A01K67/027
CPCA61D1/00A01K67/027A01K2207/30A01K2227/106A01K2267/03
Inventor 陈刚刘萧袁红江杨楠曾佳
Owner 澎立生物医药技术(上海)股份有限公司
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