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Method for comprehensively utilizing procambarus clarkia by-products

A technology of Procambarus clarkii and by-products, which is applied in the field of comprehensive utilization of Procambarus clarkii by-products, can solve problems such as a reasonable process that does not comprehensively consider the simultaneous utilization of several substances, achieve moderate calcium content, high solubility, and avoid pollution Effect

Active Publication Date: 2014-01-01
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although a lot of research has been done on shrimp by-product resources, they mainly focus on the extraction of protein, inorganic salts, astaxanthin, chitin and other components in shrimp by-products, without comprehensive consideration of the simultaneous utilization of several substances. Reasonable workmanship

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0023] Example 1: Clean the head and shell of Procambarus clarkii, dry in an electric blast drying oven at 45°C for 30 hours, pulverize with a high-speed traditional Chinese medicine grinder, pass through a 60-mesh sieve, and store at -18°C to prepare Shrimp powder; the mass percentage concentration is that 22% citric acid solution and the mass percentage concentration are 28% malic acid solution and is equal to the ratio of 1:1.5 by volume to prepare mixed acid, according to shrimp powder (g): mixed acid (mL): Distilled water (mL) = 1:3.2:54 was mixed in a ratio of 1:3.2:54, extracted to obtain an extract; ultrasonic-assisted extraction: under the conditions of ultrasonic power 250W and ultrasonic temperature 55°C, ultrasonically treat the obtained extract for 55 minutes, filter , to obtain the leaching filter residue and the leaching filtrate, and the leaching filtrate was vacuum concentrated and dried to obtain citric acid-malate calcium powder; the leaching filter residue w...

Embodiment 2

[0024] Example 2: Wash the shrimp heads and shells of Procambarus clarkii, dry them in an electric blast drying oven at 50°C for 28 hours, pulverize them with a high-speed traditional Chinese medicine grinder, pass through a 60-mesh sieve, and store them at -18°C to prepare Shrimp powder: citric acid solution with mass percentage concentration of 20% and malic acid solution with mass percentage concentration of 30% are prepared into a mixed acid in a volume ratio equal to 1:2, according to shrimp powder (g): mixed acid (mL): Distilled water (mL) = 1:3:50 was mixed for leaching to obtain an extract solution; ultrasonic-assisted extraction was performed on the obtained extract solution: under the conditions of ultrasonic power 200W and ultrasonic temperature 50°C, ultrasonic treatment was performed for 55 minutes. Filtrate to obtain the leaching filter residue and leaching filtrate, the leaching filter residue is used to extract protein and astaxanthin, the leaching filtrate is v...

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Abstract

The invention relates to a method for comprehensively utilizing procambarus clarkia by-products, which comprises the following steps of: preparing heads and shells of procambarus clarkias into shrimp powder at first; leaching and extracting the shrimp powder by using mixed acid prepared from citric acid solution and malic acid solution and distilled water; carrying out auxiliary extraction of leach liquor through ultrasonic wave, and drying filtered filtrate in vacuum to obtain citric acid-calcium malate; washing leached residues by using water to be neutral, and drying; carrying out enzymolysis of the leached residues by using compound protease prepared by mixing papain and flavourzyme and distilled water, deactivating enzyme after carrying out enzymolysis, centrifuging to obtain supernate and precipitate, and concentrating, freezing and drying supernate to obtain protein powder; and extracting and filtering the precipitate by using extractant prepared by mixing pure ethyl acetate and absolute ethyl alcohol, and concentrating, freezing and drying filtrate in vacuum to obtain astaxanthin powder. According to the invention, calcium, protein and astaxanthin in procambarus clarkia by-products are extracted step by step; the environmental pollution problem of shrimp enterprises is well solved; and resource saving is realized to the most extent.

Description

technical field [0001] The invention relates to a comprehensive utilization of by-products of shrimp, in particular to a method for comprehensive utilization of by-products of Procambarus clarkii. Background technique [0002] Procambarus clarkii ( Procambarus clarkii ), commonly known as freshwater crayfish or red marsh crayfish, is one of the main freshwater shrimps in my country. Shrimp by-products mainly refer to the leftovers of shrimps, shrimp tails and whole limbs after screening raw shrimps, including low-value shrimps, shrimp shells, shrimp legs and shrimp heads that cannot be processed and utilized. About 85% of these by-products are rich in protein, fat and minerals, as well as active ingredients such as astaxanthin and chitin. Shrimp head shell waste contains animal protein 20%-30%, inorganic salt 30%-40%, shrimp shell contains chitin 20%-30% and astaxanthin 4%-8%. Shrimp head and shell waste produced by shrimp processing in my country accounts for about 85% o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C59/245C07C59/265C07C51/41C07C403/24C12P21/06
CPCC07C51/412C07C403/24C12P21/06C07C59/245C07C59/265
Inventor 邓放明赵玲艳王燕
Owner HUNAN AGRICULTURAL UNIV
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