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Fermenting bed bioactive padding and preparation and application methods thereof

A bioactive, fermentation bed technology, applied in biochemical equipment and methods, microorganism-based methods, applications, etc., can solve problems such as imperfection and lack of bedding formula, and achieve pollution improvement, elimination of non-point source pollution, and good results. Effect

Active Publication Date: 2013-04-24
湖南省微生物研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the technology of raising pigs in fermented bed is not perfect at present. In the demonstration of this technology, outstanding problems such as lack of litter formula with wide sources and good degradation effect have been encountered.

Method used

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  • Fermenting bed bioactive padding and preparation and application methods thereof
  • Fermenting bed bioactive padding and preparation and application methods thereof
  • Fermenting bed bioactive padding and preparation and application methods thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Embodiment 1: the preparation of fermentation agent

[0062] 1) Preparation of fermentation product of Bacillus subtilis CGMCC1.1413

[0063] First, the Bacillus subtilis is activated on a slant, and the shake flask seeds are cultivated;

[0064] Then carry out fermenter culture: the medium loading in the fermenter is 40% to 50% of the total volume, the inoculation amount accounts for 0.1% to 0.3% of the medium volume, the fermentation temperature is 35°C to 37°C, and the air is stirred at 200rpm to 220rpm For cultivation, the ratio of aeration volume to fermentation broth volume is 1:1~1.1:1, tank pressure is 0.03MPa~0.05MPa; the end point of fermentation is that the number of spores is not less than 90% of the total number of bacteria, and the number of viable bacteria in the fermentation broth is 2.0 ×10 10 cfu / mL~2.2×10 10 Between cfu / mL; the mass percentage of each component of the fermenter medium is: glucose 0.4% to 0.6%, starch 0.3% to 0.5%, bean cake powder ...

Embodiment 2

[0074] The preparation of embodiment 2 fermented bedding material

[0075] (1) Expose sawdust, rice husk and straw to the sun for 1-2 days;

[0076] (2) Cut the straw into 1-2cm;

[0077] (3) Weigh each raw material according to the following weight:

[0078] 32kg of sawdust, 43kg of rice husk, 22kg of straw, 2.8kg of brown sugar, 0.2kg of compound microbial fermentation agent (the fermentation product prepared in Example 1 is mixed with protease, wherein the effective live bacteria content is 8.0×10 9 cfu / g, protease activity is 35U / g. The bacterial count percentage of Bacillus subtilis is 60%, and the bacterial count percentage of Lactobacillus casei is 40%. )

[0079] (4) Fully mix the brown sugar with the fermenting agent and dilute the fermenting agent;

[0080] (5) Mix the sawdust, rice husk and straw evenly in the fermentation tank, then mix the dilution of the fermentation agent with the above litter mixture evenly, and control the moisture content at about 50%;

...

Embodiment 3

[0082] The preparation of embodiment 3 fermented bedding material

[0083] (1) Expose sawdust, rice husks, and cotton stalks to the sun for 1-2 days;

[0084] (2) Crush the cotton stalk;

[0085] (3) Weigh each raw material according to the following weight:

[0086] Sawdust 36kg, rice husk 40kg, cotton stalk 21kg, brown sugar 2.7kg, compound microbial fermentation inoculant 0.3kg (the fermented product prepared in embodiment 1 mixes with protease, and wherein effective live bacteria content is 7.7 * 10 9 cfu / g, protease activity is 37U / g. The bacterial count percentage of Bacillus subtilis is 50%, and the bacterial count percentage of Lactobacillus casei is 50%. )

[0087] (4) Fully mix the brown sugar with the fermenting agent and dilute the fermenting agent;

[0088] (5) Mix the sawdust, rice husk and cotton stalk powder evenly in the fermentation tank, and then mix the diluted fermentation agent with the above litter mixture evenly again, and the moisture content of t...

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Abstract

The invention discloses fermenting bed bioactive padding and preparation and application methods thereof. The fermenting bed bioactive padding is made from, by weight percent, 20%-50% of sawdust, 20%-50% of rice hull, 20%-30% of crop straw, 2%-3% of accessories, and 0.1%-0.3% of fermenting agent. The fermenting bed bioactive padding has the main function of effectively degrading pen manure to improve pollution of rural environment in pig industry. The powerfully functional beneficial microorganisms in the fermenting bed padding are capable of absorbing, degrading and digesting excrement of pigs during breeding, so that pig manure is degraded in situ in a fermenting bed and area source pollution of the rural environment in the pig industry is eliminated.

Description

technical field [0001] The invention relates to a biologically fermented bedding material, in particular to a biologically fermented bedding material used for pig breeding. The invention also relates to a preparation method and application of the fermented bedding material, belonging to the field of pig breeding. Background technique [0002] In recent years, with the rapid development of large-scale and intensive pig farming in my country, the pollution of pig manure and sewage to the environment has become increasingly serious. Fermentation bed pig raising is a new pig raising technology introduced from Japan and other countries in recent years. This technology is based on the principle of microecology and microbial fermentation theory. and other auxiliary starters are mixed evenly in a certain proportion, spread in the piggery fermentation tank, and naturally fermented to form a microbial fermentation bed, using the microorganisms in the fermentation bed to "in situ degra...

Claims

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Application Information

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IPC IPC(8): A01K1/015C12N11/02C12N1/20C12R1/125C12R1/245
Inventor 尹红梅贺月林张德元许丽娟王震陈薇吴迎奔丁祥力刘标
Owner 湖南省微生物研究所
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