Anti-rice blast gene Pi9 specific CAPS marker Pi9caps and application thereof

Abstract

The invention discloses an anti-rice blast gene Pi9 specific CAPS marker Pi9caps and application thereof. The invention discloses a method for identifying or auxiliarily identifying whether rice contains anti-rice blast gene Pi9 and / or whether rice has rice blast resistance, which comprises the following steps: detecting 40804th nucleotide from the 5' terminal of 75-1-127BAC12.1 sequence or homologous sequence thereof in chromosome 6 of rice to be detected, and identifying according to the kind of the 40804th nucleotide the 5' terminal of the 75-1-127BAC12.1 sequence or homologous sequence thereof in chromosome 6 of rice. The anti-rice blast gene Pi9 specific CAPS marker Pi9caps can accurately judge whether the selected rice plant contains the anti-rice blast gene Pi9, and thus, has important application meanings in accelerating the anti-rice blast rice species breeding progress.

Description

technical field [0001] The invention relates to a specific CAPS marker Pi9caps of the rice blast resistance gene Pi9 and an application thereof, belonging to the field of crop molecular genetics and breeding. Background technique [0002] Rice is one of the most important food crops in the world, and more than half of the world's population uses rice as a staple food. Rice blast is the main disease that affects rice production, ranking first among fungal diseases. Rice blast causes a 10% to 30% reduction in rice production worldwide every year, and in severe cases, a 40% to 50% reduction in production, or even no harvest. Practice has proved that the use of rice blast resistance genes is the most economical, effective and environmentally friendly method to control rice blast disease. So far, at least 68 rice blast resistance loci and 83 major genes have been reported in rice. These genes are distributed in clusters on all rice chromosomes except chromosome 3, and 24 have be...

AI Technical Summary

Problems solved by technology

In addition, since many restriction endonucleases can participate in the digestion of amplified DNA, the chance of detecting polymorphisms is greater

[0004] It is very difficult to use the conventional field phenotype to identify the selection of the rice blast resistance gene, the selection efficiency is very low, and the selection accuracy is very poor. However, the use of molecular markers linked to the rice blast resistance gene or the specific marker selection of the gene itself is very difficult in production. Proven to be very effective and economical

For the selection of molecular markers of the Pi9 gene, researchers have developed some markers, but they all have some problems or inconveniences in application. There is a possibility of exchange with the Pi9 gene during the division process, so that the selection is not accurate enough; there are currently two reports of specific molecular markers for the Pi9 gene itself, one is a 10bp insertion / deletion site for the Pi9 promoter region (Chen Songbiao et al. , 2013), another example is the dCAPS marker for the difference in the 18bp restriction fragment of the Pi9 coding region (Huaxia et al., 2013), but due to the small size of the difference between the two markers, non-denaturing polyacrylamide gel electrophoresis is usually required to detect the difference. distinction, so there are some cumbersome operations during the operation

Images