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Salt-tolerant microbial agent and preparation method thereof

A microbial inoculum and salt-resistant technology, which is applied in the field of environmental microorganisms to achieve the effects of good impact resistance, strong impact resistance, and improved wastewater treatment effect.

Active Publication Date: 2013-10-30
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition to solving the problems existing in the existing short-range nitrification and denitrification, the microbial agent can be added to various biochemical reaction structures to improve the composition of microorganisms and increase the efficiency of nitrogen removal. of organic pollutants

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The preparation method of embodiment 1 microbial bacterial agent

[0027] 1. Bacterial activation: The medium formula used for bacterial activation and seed liquid cultivation of Staphylococcus kosheri FSDN-C is: beef extract: 6g / L, peptone: 8g / L, NaNO 2 : 0.6g / L, methanol: 0.5mL / L; the activation medium formula of Arthrobacter FDN-1 and Flavobacterium waterii FDN-2 is: beef extract: 5g / L, peptone: 10g / L, NaNO 2 : 1g / L, add 2.0% agar; the activation medium formula of denitrification paracoccus DN-3 is: KNO 3 : 1g / L, sodium succinate: 8g / L, KH 2 PO 4 :1g / L, FeCL 2 : 0.5g / L, add 2.0% agar; the activation medium formula of Methylobacterium SDN-3 is: ammonium sulfate: 0.5g / L, methanol: 0.75mL / L, KH 2 PO 4 :1g / L, FeCL 2 :0.5g / L; add 2% agar. After spreading evenly on the plate, place it in a constant temperature incubator at a temperature of 30°C for activation.

[0028] 2. Use the inoculation loop to scrape the bacteria on the plate and inoculate them in the corre...

Embodiment 2

[0031] Example 2 Microbial agent treatment of salty wastewater containing ammonia and nitrogen catalysts

[0032] The water quality characteristics of a chemical plant's salty wastewater containing ammonia and nitrogen catalysts: pH8.91, conductivity 5.8mS / cm, COD 530mg / L, ammonia nitrogen 237mg / L. During the long-term operation of the system, the removal effect of ammonia nitrogen is poor, resulting in a total nitrogen concentration of up to 182mg / L, the SVI of activated sludge is greater than 200 mL / g, and the sedimentation performance is poor. Add a certain amount of liquid bacterial suspension A to the system at 1% (volume ratio) every day for a total of 10 days. The SVI in the detection system is less than 200mL / g. After one month of operation, the ammonia nitrogen in the effluent after analysis and treatment is lower than 15mg / L, and the total nitrogen is lower than 25mg / L. It can be seen that the microbial bacterial agent can improve the treatment effect of saline an...

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Abstract

The invention discloses a salt-tolerant microbial agent and a preparation method thereof. The microbial agent contains staphylococcus cohnii FSND-C, arthrobacter creatinolyticus FDN-1, flavobacterium mizutaii FDN-2, paracoccus denitrificans DN-3 and methylobacterium phyllosphaerae SDN-3. The microbial agent can achieve removal of ammonia nitrogen, total nitrogen and CODcr in the same reactor, has a good wastewater treatment effect, and can achieve short-cut nitrification and denitrification or simultaneous nitrification and denitrification while removing COD. Staphylococcus cohnii FSND-C can utilize various carbon sources, has certain salt tolerance, can be applied to the high-salinity wastewater treatment process, simultaneously can secrete a substance under environmental stimulus to enhance the flocculability of sludge, and further widens the application range of the microbial agent.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and in particular relates to a microbial bacterial agent for wastewater treatment and a preparation method thereof. The bacterial agent can complete the removal of ammonia nitrogen, total nitrogen and CODcr, and realize the industrialization and production of microbial bacterial agents for wastewater treatment. commoditization. Background technique [0002] Biological denitrification is one of the more cost-effective methods to solve nitrogen pollution. Whether it is a traditional microbial-attached wastewater treatment structure or a newly developed high-efficiency biofilm treatment system, the microorganisms responsible for nitrogen removal are mainly autotrophic nitrifying bacteria. The proliferation rate of autotrophic bacteria is slow, they cannot compete with heterotrophic bacteria in the mixed culture activated sludge system, it is difficult to obtain high biomass, and the nitr...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/20C02F3/34C12R1/01C12R1/06C12R1/20
Inventor 高会杰孙丹凤许谦
Owner CHINA PETROLEUM & CHEM CORP
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