42 results about "Methylobacterium organophilum" patented technology

The invention relates to a degrading bacterium Methylobacterium sp. YWF1 capable of degrading three TC (tetracycline) antibiotics, namely, TC, OTC (oxytetracycline) and CTC (Chlorotetracycline) simultaneously. The degrading bacterium YWF1 is identified as Methylobacterium and was preserved in CGMCC (China General Microbiological Culture Collection Center) on April 17, 2017 with preservation number being CGMCCNo. 14040. Methylobacterium sp. YWF1 can simultaneously degrade the three TC antibiotics, namely, TC, OTC and CTC, a microbial agent suspension can be prepared from the bacterium to be applied to degrading removal of TC antibiotic pollutants in different environment media such as livestock wastewater, TC pharmaceutical enterprise wastewater, medical wastewater or soil, and has good industrial application prospect and environmental benefits.

The invention discloses a microbial agent for efficiently degrading formaldehyde and a preparation method of the microbial agent, belongs to the technical field of indoor air purification, and solvesthe technical problem that an existing single microbial agent cannot efficiently degrade low-concentration formaldehyde. The preparation method of the microbial agent comprises the following steps: S1, activating Methylobacterium, Pseudomonas and Rhodopseudomonas palustris for standby application; S2, inoculating the activated Methylobacterium and activated Pseudomonas into a liquid culture mediumA for culturing for 12-18 h, then inoculating the activated Rhodopseudomonas palustris into the liquid culture medium A, and performing culturing in a dark environment with formaldehyde; and S3, centrifuging the microbial culture solution, and performing cell disruption, membrane filtration and drying on thalli to obtain the microbial agent. The microbial agent disclosed by the invention is usedfor degrading indoor formaldehyde, and has the advantage of efficiently degrading indoor low-concentration formaldehyde.

Methylotrophic or methanotrophic bacteria such as Methylobacterium are transformed with a gene of interest, and expression of the gene is regulated by means of a cumate repressor protein and an operator sequence which is operatively linked to the gene of interest, and the addition of an external agent. Specifically, the cymR repressor and cmt operator from Pseudomonas putida may serve to regulate gene expression in methylotrophic or methanotrophic bacteria with the addition of cumate.

The present invention provides methods for the cultivation of the Methylobacterium genus of bacteria. In particular the method provides methods for the efficient and inexpensive cultivation of these bacteria. Additionally, the invention provides methods for the utilization of these bacterial cultures to improve plant agriculture.

The invention discloses a salt-tolerant microbial bacterial agent and a preparation method thereof. The microbial bacterial agent contains Staphylococcus coli FSDN-C, Arthrobacter FDN-1, Flavobacterium waterii FDN-2, Paracoccus denitrogeni DN-3 and Methylobacterium sdn-3. The microbial bacterial agent can realize the removal of ammonia nitrogen, total nitrogen and CODcr in the same reactor, has good wastewater treatment effect, and can realize short-range nitrification and denitrification or simultaneous nitrification and denitrification denitrification while removing COD. Staphylococcus koshii FSDN-C can utilize a variety of carbon sources and has a certain salt tolerance, which can be used in the treatment of high-salt wastewater. At the same time, the strain can secrete a substance to enhance sludge flocculation under environmental stimulation sex. This further broadens the scope of application of the microbial agent.

The invention discloses an isolated methylbacterium sp. DM1, which can decompose N, N-Dimethyl formamide. Methylbacterium sp. DM1 can use DMF as the sole carbon and nitrogen source to grow, mineralizeit thoroughly and release ammonia nitrogen. It can tolerate and completely degrade 6800 mg / L DMF in water. Under the condition of 30 DEG C and pH 7.0, 2000 mg / L DMF, methylbacterium sp. DM1 can be degraded completely within 28 hours, and the degradation rate reaches 71.4 mg / L / h, which is one of the fastest reported strains. Methylbacterium sp. DM1 has a good application prospect in environmentalsewage treatment and environmental remediation.

Disclosed are methods of producing carotenoid compounds in a methylotrophic bacterial host cell. Such a host cell may be an unmodified Methylobacterium, spontaneous mutant, or transformed cell, any of which exhibit favorable properties, such as overproduction of carotenoid compounds, increased carbon flux, improved growth, or the production of additional nutrients, such as protein, vitamins, antioxidants, or fatty acids. Also disclosed are feed compositions for use in aquaculture, or as animal feed, or as human nutritional supplements containing processed or unprocessed biomass from such cells, as are methods of preparation of the feed compositions.

The application discloses a microbial inoculant for efficiently degrading formaldehyde and a preparation method thereof, belonging to the technical field of indoor air purification, and solving the technical problem that the existing single microbial inoculant cannot efficiently degrade low-concentration formaldehyde. The preparation method of microbial inoculum comprises the following steps: S1, activate Methylobacterium, Pseudomonas and Rhodopseudomonas swamp, for standby use; S2, inoculate the activated Methylobacterium and the activated Pseudomonas into a liquid After culturing in medium A for 12-18 h, inoculate the activated Rhodopseudomonas swamp into liquid medium A, and cultivate in a dark environment with formaldehyde; S3, centrifuge the microbial culture solution, and remove the bacterial cells The microbial inoculum is obtained after the cells are broken, passed through the membrane and dried. The microbial inoculum of the present application is used for indoor formaldehyde degradation, and has the advantage of efficiently degrading indoor low-concentration formaldehyde.

The invention discloses a wastewater treatment microbial agent and a preparation method thereof. The microbial agent contains Kocuriapalustris FSDN-A, Arthrobacter creatinolyticus FDN-1, Flavobacterium mizutaii FDN- 2. Paracoccus denitrificans DN-3 and Methylobacteriumphyllosphaerae SDN-3, the deposit registration numbers of the five strains are CGMCC No.5061, CGMCC No.3657, CGMCC No.3659, and CGMCC No. 3658 and CGMCC No.3660. The microbial bacterial agent can realize the removal of ammonia nitrogen, total nitrogen and CODcr in the same reactor, has good wastewater treatment effect, and can realize short-range nitrification and denitrification or simultaneous nitrification and denitrification denitrification while removing COD. Corkia palustris FSDN-A can utilize two kinds of nitrogen oxides, nitrous nitrogen and nitrate nitrogen, which can make the wastewater treatment system denitrify more thoroughly, and the value-added speed is slow in the process of competing for substrates, which is conducive to the reduction of sludge. Further broaden the scope of application of microbial agents.

The invention discloses an isolated Methylobacterium sp. DM1 (Methylobacterium sp. DM1), which can decompose N,N-dimethylformamide. Methylobacterium DM1 can use DMF as the only carbon and nitrogen source to grow, completely mineralize it and release ammonia nitrogen. It can tolerate and completely degrade 6800mg / L of DMF in water. Under the conditions of 30°C and pH 7.0, 2000mg / L DMF can be completely degraded within 28 hours, and the degradation rate reaches 71.4mg / L / h, which is one of the fastest reported strains. It is used in environmental sewage treatment and It has a good application prospect in environmental restoration.

The invention provides a Methylorubrum rhodesianum M520, which is preserved in the Guangdong Microbial Culture Collection Center with a preservation number of GDMCC No.60729. The present invention also provides the application of the M520 bacterial strain in preparing and preventing root-knot nematodes or promoting plant growth. The crude extract of metabolism of Methylorubrum rhodesianum M520 strain of the present invention acts on root-knot nematodes, and the lethal rate to root-knot nematodes exceeds 96.6%; At the same time, it can significantly promote the growth of vegetable plants, expand the application range and efficacy of Methylobacterium, enrich the prevention and control methods of root-knot nematode disease, and improve the growth speed and vegetable output of vegetable plants.

The invention discloses a bacterium for degrading a herbicide imazethapyr and application of the bacterium. The bacterium for degrading imazethapyr is methylobacterium sp. PST1991, which is preserved in China General Microbiological Culture Collection Center by a preservation number of CGMCC No.7773. The methylobacterium sp. PST1991 CGMCC No.7773 reaches a degrading rate of 71.06% on 8mg / L of imazethapyr in an organic salt culture medium within 7d, showing that the strain can effectively degrade the imazethapyr; the bacterium has a wide application prospect in an aspect of repairing imazethapyr pollution of soil.

The invention relates to a biological denitrification method for salt-containing sewage, which comprises the following steps: adding a denitrification microbial inoculant into a biochemical sewage treatment system, and simultaneously starting the nitrification-denitrification biological denitrification treatment processes, wherein the sewage treatment temperature is 18-40 DEG C, the dissolved oxygen is 0.1-5 mg / L, and the pH value is 7.0-9.0. The denitrification microbial inoculant contains one or two of Staphylococcuscohnii FSDN-C, Arthrobactercreatinolyticus FDN-1 and Flavobacteriummizutaii FDN-2, and also contains one or two of Paracoccusdenitrificans DN-3 and Methylobacteriumphyllosphaerae SDN-3. The method provided by the invention can effectively treat high-salt-content sewage, enhances the adsorptivity and flocculence for sludge, has wide application range for wastewater quality and high impact resistance to salt-containing sewage, and obviously enhances the sewage treatment effect on the premise of removing pollutants, such as ammonia nitrogen, COD (chemical oxygen demand) and the like.

The invention discloses a denitrification method of wastewater generated in the process of producing catalytic cracking catalysts. The method comprises the step of adding nitrobacteria and a denitrification agent to sewage, wherein the denitrification agent contains arthrobactercreatinolyticus FDN-1, flavobacteriummizutaii FDN-2, paracoccusdenitrificans DN-3 and methylobacteriumphyllosphaerae SDN-3. The method adopts autotrophic bacteria and heterotrophic bacteria which are subjected to combination of superiority to serve as reinforced microorganisms for wastewater treatment, adopts different addition modes, can achieve removal of ammonia nitrogen, total nitrogen and CODcr in the same reactor, has a good wastewater treatment effect, truly achieves short-cut nitrification and denitrification or simultaneous nitrification and denitrification of ammonia-containing catalyst wastewater by being combined with high-efficiency devices, and achieves up-to-standard discharge of pollutants such as ammonia nitrogen and the like in the wastewater.

The invention relates to a butadiene producing strain taking methyl alcohol as a raw material and a method for producing butadiene, and belongs to the technical field of construction of engineering bacteria. The butadiene producing strain belongs to methylobacterium, the producing strain belongs to methylobacterium, and the producing strain is obtained by that foreign genes of the following enzymes are expressed by methylobacterium and then transferred into modification and expression, wherein the following enzymes include butadiene synthetase MTS or ISPS, crotyl alcohol mono-phosphate kinaseIPK, crotyl alcohol kinase THK or MK or TK an coenzyme A reductase FAR or BLD-BDH or ADHE2; and methylobacterium contains a serine circulation route and an acetyl malonyl-CoA route. The butadiene producing strain provided by the invention can take methyl alcohol as the raw material to produce butadiene, and can sufficiently utilize the raw material of methyl alcohol which has the competitive priceand is sufficiently supplied, and a butadiene product is directly synthesized through microbial metabolism. The cost is greatly reduced, and achievement of industrialized production of the procedurethat butadiene is synthesized by methyl alcohol is facilitated.

The invention relates to the field of biotechnology, in particular to a Methylobacter thermophilus. The present invention provides a Methylobacter thermophilus, the deposit number of which is CGMCC No.20326. The M. thermophilus provided by the invention can use the heat of self-fermentation of compost raw materials as a heat source to reproduce and grow, and the maximum growth temperature is as high as 77°C or more, can rapidly multiply in a high-temperature composting environment, degrade organic matter, promote the composting process, and achieve thermal energy. The dual effect of utilization and waste disposal. In addition, the inoculum prepared by this strain has a low moisture content, which is beneficial to storage and transportation. It can be directly mixed with the stacking material when used. It is easy to operate and will not increase the moisture content of the material. It is also a good wet-based material conditioner. , has a wide range of application value.

The invention discloses a resveratrol composite microbial agent for treating high concentration PAHs wastewater and a usage method thereof. The composite microbial agent comprises: a reagent I and a reagent II; the reagent I is: an methylobacterium suspension diluted with an inorganic salt culture medium; the reagent II is: a mixed solution of an inorganic salt and resveratrol; the usage method includes the following steps: step 1. culturing and acclimating methylobacterium; step 2. preparing the methylobacterium suspension; step 3. preparing a resveratrol solution; step 4. uniformly mixing wastewater, the methylobacterium, and the resveratrol solutionin proportion; and step 5. filtering out water; and the microbial agent and the usage method find that resveratrol can significantly promotedegradation of activity of a PAHs key enzyme in an endophytic body, thereby promoting degradation and removal of PAHs in water by endophytes; and the microbial agent and the usage method provide a new method with high efficiency and simple operation for efficient biological treatment of the high concentration PAHs wastewater.

The present invention relates to a methylobacterium ( Methylobacterium zatmanni strain ) MR1, whose preservation number in the China Typical Culture Collection Center is CCTCC M 2017322, the methylobacteria of the present invention is made of activated sludge embedded in formaldehyde domesticated sodium alginate, and the inorganic salt culture medium of methylobacteria A methylotrophic bacterium isolated can grow in a medium with formaldehyde as the only carbon source, and its resistance to formaldehyde on a solid medium is as high as 30mM. After adding 5mM methanol and 2-15 mM formaldehyde It grows normally in the liquid medium, and has a high removal rate for the high concentration of formaldehyde in the medium. MR1 can also grow in the inorganic salt medium with sodium acetate, sodium malate or sodium citrate as the sole carbon source, among which the growth is best when sodium malate is used as the carbon source. When growing in a medium containing sodium acetate or sodium malate or sodium citrate, the added benzene series can be removed, and the removal rate can reach 100%.

Compositions comprising Methylobacterium having antifungal activity, methods of controlling phytopathogenic fungi, and methods of making the compositions are provided.