Application of rice GT transcription factor family gene OsGT gamma-1 in controlling salt tolerance of rice

Abstract

The invention relates to the technical field of rice genetic engineering, in particular to the application of a GT transcription factor family gene OsGT gamma-1 in controlling the salt tolerance of rice. By functional analysis and identification, the gene OsGT gamma-1 for controlling the salt tolerance of rice is obtained, and the application of the gene in adverse resistance genetic improvement of the salt tolerance of rice is verified. The gene OsGT gamma-1 is one of the following nucleotide sequences: 1) the nucleotide sequence shown from 475th to 1707th in a sequence table SEQ NO: 1; or the nucleotide sequence of protein with the same code as that of the protein encoded by 1). The nucleotide sequence containing the gene OsGT gamma-1 is transferred to rice after being connected with an exogenous promoter, and the salt tolerance of the transgenic rice is obviously enhanced.

Description

technical field The invention relates to the field of rice genetic engineering. It specifically involves isolating and cloning a rice GT transcription factor family gene OsGTγ-1 that can improve high-salt tolerance, and realizing its application in genetic improvement of rice stress resistance through functional verification. The present invention uses the method of screening T-DNA insertion rice mutant library to isolate and clone the gene OsGTγ-1 that controls the salt tolerance of rice. On the one hand, it is verified that the mutant is closely linked with the high-salt sensitive phenotype through co-segregation detection, and on the other hand On the one hand, the overexpression of OSGTγ-1 gene improves the tolerance of transgenic rice to high salt, confirming the function of the gene. Background technique The growth and development of plants not only depends on their genetic background, but also is affected by many environmental factors. The environment for plant grow...

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Problems solved by technology

Rice GT-2 and Arabidopsis GT-1 can activate transcription in vivo (Le et al., Transcriptional activation by Arabidopsis GT-1 may be through interaction with TF II A-TBP-TATA complex. Plant J.1999, 18:663-668 ; Ni et al., GT-2: in vivo transcriptional activation activity and definition of novel twin DNA binding domains with reciprocal target sequence selectivity. Plant Cell. 1996, 8: 1041-1059), Arabidopsis thaliana PTL is involved in regulating the development of floral organs (Brewer et al., PETAL LOSS, a trihelix transcription factor gene, regulates perianth architecture in the Arabidopsis flower. Development. 2004, 131: 4035-4045), but whether this family is involved in the stress response of plants has not yet been reported

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