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Method for identifying transgenic rice and non-transgenic rice based on NIR (Near Infrared Spectrum)

A near-infrared spectroscopy and transgenic rice technology, which is applied in the field of detection of transgenic plants, can solve the problems of complicated operation steps, complicated detection methods, long time-consuming and the like, and achieves the effects of high identification accuracy, non-destructive identification and simple operation.

Inactive Publication Date: 2012-12-26
CHINA JILIANG UNIV
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Problems solved by technology

[0005] The invention patent application with the application publication number CN 102081075A discloses a method for identifying transgenic rice and non-transgenic rice, including: (1) respectively measuring shikimic acid and The content of galacturonic acid; (2) if the content of shikimic acid and galacturonic acid of the rice to be detected is significantly lower than the content of shikimic acid and galacturonic acid of non-transgenic rice, the rice to be detected is transgenic Rice; due to the complicated detection methods of shikimic acid and galacturonic acid, the operation steps of this invention are cumbersome and time-consuming

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  • Method for identifying transgenic rice and non-transgenic rice based on NIR (Near Infrared Spectrum)

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Embodiment 1

[0028] 1. Build a model

[0029] (1) Select 80 transgenic rice seeds and 40 non-transgenic rice seeds as rice seed samples, and use a Nicolet Nexus870 (Thermo Corporation USA) Fourier transform near-infrared spectrometer to emit wavenumbers ranging from 4000 to 10000 cm to the rice seed samples -1 The near-infrared spectrum, using OMNIC 6.0 software to collect the diffuse reflectance spectra of all rice seed samples; set the number of scans of the near-infrared spectrometer to 32 times, with a resolution of 4cm -1 . During collection, the room temperature was controlled at about 25°C, and the humidity was kept stable.

[0030] The above-mentioned transgenic rice is TCTP gene transgenic rice and Osmi166 gene transgenic rice, and its acquisition method can be: the transgenic material adopts the mature embryo of rice respectively as explant induction, cultures callus, selects embryogenic callus as transformation recipient, Transgenic rice was obtained by transforming Agrobacter...

Embodiment 2

[0050] 1. Build a model

[0051] (1) With embodiment 1.

[0052] (2) Use the standard normal transformation method to preprocess the diffuse reflectance spectra of all rice seed samples to obtain the preprocessed spectral information; use the principal component analysis method in the Unscrambler software to extract the wave number range after each pretreatment 4000~8000cm -1 For the spectral feature information in the spectral section, the number of principal components is selected as 5, and the scores of each principal component are obtained (as shown in Table 4);

[0053] (3) Take the principal component scores corresponding to the spectral information of all rice seed samples as input, and use the rice seed type setting value corresponding to the rice seed sample as the output to establish the PLS-DA model; the rice seed type setting corresponding to the rice seed sample The values ​​were set as follows: TCTP-transgenic rice and mi166-transgenic rice were set to -1, and ...

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Abstract

The invention discloses a method for identifying transgenic rice and non-transgenic rice based on NIR (Near Infrared Spectrum), which comprises the following steps of: (1) transmitting the NIR to the rice seed samples, and collecting the diffuse reflection spectrum information of all the rice samples; (2) respectively preprocessing the diffuse reflection spectrum information for all the rice seed samples, extracting the spectrum information in characteristic spectrum regions after preprocessing through a principal component analysis method, selecting principal components, and acquiring the scores of the principal components; (3) building a model by the principal component scores corresponding to the rice seed sample spectrum information as an input and the rice seed type set values corresponding to the rice seed samples as an output; and (4) acquiring the scores of the principal components of the spectrum of rice seeds to be detected, taking the scores into the model in the step (3), and obtaining the types of the rice seeds to be detected. The invention is high in identification precision, simple to operate, low in cost, and is capable of realizing quickly and losslessly identifying the transgenic rice and the non-transgenic rice.

Description

technical field [0001] The invention belongs to the detection field of transgenic plants, and in particular relates to a method for discriminating transgenic rice and non-transgenic rice based on near-infrared spectroscopy. Background technique [0002] Rice is the largest grain crop in my country, and its annual output accounts for about 38% of the total grain output. Its production is related to the food security of the country. Transgenic technology breaks through the limitations of traditional rice breeding and provides a new way to ensure my country's food security. With the rapid development of rice genetic engineering technology, the research results of genetically modified rice are remarkable. In the past 20 years, the use of transgenic technology has successfully developed insect-resistant, disease-resistant, herbicide-resistant, stress-resistant, high-yield and high-quality transgenic rice, and obtained a large number of genetically modified rice lines with stable...

Claims

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Application Information

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IPC IPC(8): G01N21/35G01N21/3563G01N21/359
Inventor 朱诚张龙丁艳菲王珊珊
Owner CHINA JILIANG UNIV
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