Qualitative PCR detecting method for transgenic insect-resistant herbicide-tolerant rice G6H1 and derived varieties thereof
A herbicide-tolerant, G6H1 technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0048] Example 1 Primer screening and optimization of PCR reaction conditions for the qualitative PCR detection method of transgenic insect-resistant and herbicide-resistant rice G6H1 and its derivatives
[0049] 1. Primer design and screening
[0050] According to the insertion sequence of G6H1 rice ( figure 1 ) to design primers, design 8 primers according to the side sequence of the 5' end of the inserted sequence, design 9 primers according to the side sequence of the 3' end of the inserted sequence, and pair the above primers respectively, and the length of the target sequence is between 100 and 400 bp , the primer sequences are shown in Table 1.
[0051] Table 1 Design primer information table
[0052]
[0053] G6H1 rice and non-transgenic rice DNA with mass fractions of 100%, 1%, and 0.1% were used as templates, and double-distilled water was used to replace the template of the PCR reaction system in the blank control. Conventional PCR reaction conditions and react...
Embodiment 2
[0061] Example 2 Establishment of Qualitative PCR Reaction System and Reaction Program of Transgenic Insect-resistant and Herbicide-resistant Rice G6H1 and Its Derivatives
[0062] According to the Tm value of the detection primer screened in Example 1 and the stability of the amplification result, determine the qualitative PCR reaction system (see Table 3) and reaction program of the transgenic insect-resistant and herbicide-resistant rice G6H1 and its derivative varieties: denaturation at 94°C for 5 minutes ; Denaturation at 94°C for 30s, annealing at 58°C for 45s, extension at 72°C for 60s, a total of 35 cycles; extension at 72°C for 7min.
[0063] Table 3 Qualitative PCR reaction system of transgenic insect-resistant and herbicide-resistant rice G6H1 and its derivatives
[0064]
experiment example 1
[0065] Experimental example 1 Specificity test of qualitative PCR detection method for transgenic insect-resistant and herbicide-resistant rice G6H1 and its derivative varieties
[0066] 1. Plant material
[0067] Transgenic rice: G6H1, Kefeng 6, Kefeng 8, Kemo rice, M12, TT51;
[0068] Other transgenic plants:
[0069] Corn: Bt11, Bt176, MON810, MON863, GA21, NK603, T25, TC1507, MON89034, 59122, MIR604, MON88017, 3272, MON87460;
[0070] Soybean: 356043, 305423, CV127, MON89788, A5547-127, A2704-12, GTS40-3-2;
[0071] Cotton: MON1445, MON531, MON15985, LLCOTTON25, MON88913; Rapeseed: MS1, MS8, RF1, RF2, RF3, T45, Oxy235, Topas19 / 2;
[0072] Non-transgenic rice Xiushui 110;
[0073] Transgenic corn, soybean, cotton and rape were purchased from Sigma-Aldrich (Shanghai) Trading Co., Ltd.; transgenic rice and non-transgenic rice Xiushui 110 were collected in this experiment; among them, the seeds of transgenic rice G6H1 were provided by the developer, Professor Zhicheng Shen...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com